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The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789.
BMC Res Notes. 2020 Aug 27; 13(1):398.BR

Abstract

OBJECTIVE

In December 2019 a novel coronavirus (SARS-CoV-2) that is causing the current COVID-19 pandemic was identified in Wuhan, China. Many questions have been raised about its origin and adaptation to humans. In the present work we performed a genetic analysis of the Spike glycoprotein (S) of SARS-CoV-2 and other related coronaviruses (CoVs) isolated from different hosts in order to trace the evolutionary history of this protein and the adaptation of SARS-CoV-2 to humans.

RESULTS

Based on the sequence analysis of the S gene, we suggest that the origin of SARS-CoV-2 is the result of recombination events between bat and pangolin CoVs. The hybrid SARS-CoV-2 ancestor jumped to humans and has been maintained by natural selection. Although the S protein of RaTG13 bat CoV has a high nucleotide identity with the S protein of SARS-CoV-2, the phylogenetic tree and the haplotype network suggest a non-direct parental relationship between these CoVs. Moreover, it is likely that the basic function of the receptor-binding domain (RBD) of S protein was acquired by the SARS-CoV-2 from the MP789 pangolin CoV by recombination and it has been highly conserved.

Authors+Show Affiliations

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. marosari@unam.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32854762

Citation

Flores-Alanis, Alejandro, et al. "The Receptor Binding Domain of SARS-CoV-2 Spike Protein Is the Result of an Ancestral Recombination Between the bat-CoV RaTG13 and the pangolin-CoV MP789." BMC Research Notes, vol. 13, no. 1, 2020, p. 398.
Flores-Alanis A, Sandner-Miranda L, Delgado G, et al. The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789. BMC Res Notes. 2020;13(1):398.
Flores-Alanis, A., Sandner-Miranda, L., Delgado, G., Cravioto, A., & Morales-Espinosa, R. (2020). The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789. BMC Research Notes, 13(1), 398. https://doi.org/10.1186/s13104-020-05242-8
Flores-Alanis A, et al. The Receptor Binding Domain of SARS-CoV-2 Spike Protein Is the Result of an Ancestral Recombination Between the bat-CoV RaTG13 and the pangolin-CoV MP789. BMC Res Notes. 2020 Aug 27;13(1):398. PubMed PMID: 32854762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789. AU - Flores-Alanis,Alejandro, AU - Sandner-Miranda,Luisa, AU - Delgado,Gabriela, AU - Cravioto,Alejandro, AU - Morales-Espinosa,Rosario, Y1 - 2020/08/27/ PY - 2020/07/01/received PY - 2020/08/19/accepted PY - 2020/8/29/entrez PY - 2020/8/29/pubmed PY - 2020/9/8/medline KW - Genealogy KW - Molecular evolution KW - Natural selection KW - Recombination KW - SARS-CoV-2 KW - Spike glycoprotein SP - 398 EP - 398 JF - BMC research notes JO - BMC Res Notes VL - 13 IS - 1 N2 - OBJECTIVE: In December 2019 a novel coronavirus (SARS-CoV-2) that is causing the current COVID-19 pandemic was identified in Wuhan, China. Many questions have been raised about its origin and adaptation to humans. In the present work we performed a genetic analysis of the Spike glycoprotein (S) of SARS-CoV-2 and other related coronaviruses (CoVs) isolated from different hosts in order to trace the evolutionary history of this protein and the adaptation of SARS-CoV-2 to humans. RESULTS: Based on the sequence analysis of the S gene, we suggest that the origin of SARS-CoV-2 is the result of recombination events between bat and pangolin CoVs. The hybrid SARS-CoV-2 ancestor jumped to humans and has been maintained by natural selection. Although the S protein of RaTG13 bat CoV has a high nucleotide identity with the S protein of SARS-CoV-2, the phylogenetic tree and the haplotype network suggest a non-direct parental relationship between these CoVs. Moreover, it is likely that the basic function of the receptor-binding domain (RBD) of S protein was acquired by the SARS-CoV-2 from the MP789 pangolin CoV by recombination and it has been highly conserved. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/32854762/The_receptor_binding_domain_of_SARS_CoV_2_spike_protein_is_the_result_of_an_ancestral_recombination_between_the_bat_CoV_RaTG13_and_the_pangolin_CoV_MP789_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05242-8 DB - PRIME DP - Unbound Medicine ER -