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Endotoxemia produces an increase in arterial but not venous lipid peroxides in sheep.
J Appl Physiol (1985). 1988 Feb; 64(2):592-8.JA

Abstract

Our purpose was to determine the effect of an endotoxin-induced lung injury on circulating lipid peroxides. We measured both malondialdehyde (MDA) and conjugated dienes (as optical density at 233 nm) in aortic and venous plasma and lung lymph in 10 unanesthetized sheep given 1 microgram/kg of Escherichia coli endotoxin. Total lipids and prostanoids 6-ketoprostaglandin F1 alpha and thromboxane B2 were also measured. Six control sheep were also studied. Animals were monitored for a 12-h period and then killed, and lung tissue MDA was determined. A two-phase endotoxin response was noted with an initial pulmonary hypertension followed by a steady-state increase in protein-rich lung lymph flow (QL) between a 3- and 6-h period. Aortic plasma MDA was significantly increased from a base line of 4.8 +/- 1.4 to 8.9 +/- 1.6 and 7.5 +/- 1.3 nmol/ml at 1 and 4 h post-endotoxin. Aortic plasma conjugated dienes increased in all 10 sheep post-endotoxin. Venous levels of both MDA and conjugated dienes were not significantly increased. Lung QL increased two- to three-fold. Lung lymph MDA increased significantly at 1 h post-endotoxin. Lymph conjugated dienes decreased. Plasma and lymph lipid peroxide levels returned to base line by 12 h in most animals. However, tissue MDA remained significantly increased in all sheep from base line of 45 +/- 9 to 85 +/- 14 nmol/g tissue. We conclude that both MDA and conjugated dienes are transiently released into aortic plasma during endotoxin-induced oxidant lung injury.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Longwood Area Trauma Center, Brigham and Women's, Beth Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3286598

Citation

Demling, R H., et al. "Endotoxemia Produces an Increase in Arterial but Not Venous Lipid Peroxides in Sheep." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 64, no. 2, 1988, pp. 592-8.
Demling RH, Lalonde C, Ryan P, et al. Endotoxemia produces an increase in arterial but not venous lipid peroxides in sheep. J Appl Physiol (1985). 1988;64(2):592-8.
Demling, R. H., Lalonde, C., Ryan, P., Zhu, D. G., & Liu, Y. P. (1988). Endotoxemia produces an increase in arterial but not venous lipid peroxides in sheep. Journal of Applied Physiology (Bethesda, Md. : 1985), 64(2), 592-8.
Demling RH, et al. Endotoxemia Produces an Increase in Arterial but Not Venous Lipid Peroxides in Sheep. J Appl Physiol (1985). 1988;64(2):592-8. PubMed PMID: 3286598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endotoxemia produces an increase in arterial but not venous lipid peroxides in sheep. AU - Demling,R H, AU - Lalonde,C, AU - Ryan,P, AU - Zhu,D G, AU - Liu,Y P, PY - 1988/2/1/pubmed PY - 1988/2/1/medline PY - 1988/2/1/entrez SP - 592 EP - 8 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 64 IS - 2 N2 - Our purpose was to determine the effect of an endotoxin-induced lung injury on circulating lipid peroxides. We measured both malondialdehyde (MDA) and conjugated dienes (as optical density at 233 nm) in aortic and venous plasma and lung lymph in 10 unanesthetized sheep given 1 microgram/kg of Escherichia coli endotoxin. Total lipids and prostanoids 6-ketoprostaglandin F1 alpha and thromboxane B2 were also measured. Six control sheep were also studied. Animals were monitored for a 12-h period and then killed, and lung tissue MDA was determined. A two-phase endotoxin response was noted with an initial pulmonary hypertension followed by a steady-state increase in protein-rich lung lymph flow (QL) between a 3- and 6-h period. Aortic plasma MDA was significantly increased from a base line of 4.8 +/- 1.4 to 8.9 +/- 1.6 and 7.5 +/- 1.3 nmol/ml at 1 and 4 h post-endotoxin. Aortic plasma conjugated dienes increased in all 10 sheep post-endotoxin. Venous levels of both MDA and conjugated dienes were not significantly increased. Lung QL increased two- to three-fold. Lung lymph MDA increased significantly at 1 h post-endotoxin. Lymph conjugated dienes decreased. Plasma and lymph lipid peroxide levels returned to base line by 12 h in most animals. However, tissue MDA remained significantly increased in all sheep from base line of 45 +/- 9 to 85 +/- 14 nmol/g tissue. We conclude that both MDA and conjugated dienes are transiently released into aortic plasma during endotoxin-induced oxidant lung injury.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/3286598/Endotoxemia_produces_an_increase_in_arterial_but_not_venous_lipid_peroxides_in_sheep_ L2 - https://journals.physiology.org/doi/10.1152/jappl.1988.64.2.592?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -