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ZnO nanoparticles induce cell wall remodeling and modify ROS/ RNS signalling in roots of Brassica seedlings.
Ecotoxicol Environ Saf. 2020 Dec 15; 206:111158.EE

Abstract

Cell wall-associated defence against zinc oxide nanoparticles (ZnO NPs) as well as nitro-oxidative signalling and its consequences in plants are poorly examined. Therefore, this study compares the effect of chemically synthetized ZnO NPs (~45 nm, 25 or 100 mg/L) on Brassica napus and Brassica juncea seedlings. The effects on root biomass and viability suggest that B. napus is more tolerant to ZnO NP exposure relative to B. juncea. This may be due to the lack of Zn ion accumulation in the roots, which is related to the increase in the amount of lignin, suberin, pectin and in peroxidase activity in the roots of B. napus. TEM results indicate that root cell walls of 25 mg/L ZnO NP-treated B. napus may bind Zn ions. Additionally, callose accumulation possibly contribute to root shortening in both Brassica species as the effect of 100 mg/L ZnO NPs. Further results suggest that in the roots of the relatively sensitive B. juncea the levels of superoxide radical, hydrogen peroxide, hydrogen sulfide, nitric oxide, peroxinitrite and S-nitrosoglutathione increased as the effect of high ZnO NP concentration meaning that ZnO NP intensifies nitro-oxidative signalling. In B. napus; however, reactive oxygen species signalling was intensified, but reactive nitrogen species signalling wasn't activated by ZnO NPs. Collectively, these results indicate that ZnO NPs induce cell wall remodeling which may be associated with ZnO NP tolerance. Furthermore, plant tolerance against ZnO NPs is associated rather with nitrosative signalling than oxidative modifications.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Molnár Á
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: molnara@bio.u-szeged.hu.
Rónavári A
Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary. Electronic address: ronavari@chem.u-szeged.hu.
Bélteky P
Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary. Electronic address: beltekyp@chem.u-szeged.hu.
Szőllősi R
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: szoszo@bio.u-szeged.hu.
Valyon E
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: emil6555@citromail.hu.
Oláh D
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: olahdora.csorvas@gmail.com.
Rázga Z
Department of Pathology, Faculty of Medicine, University of Szeged, H-6725, Szeged, Állomás u. 2., Hungary. Electronic address: razgazst44@hotmail.com.
Ördög A
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: aordog@bio.u-szeged.hu.
Kónya Z
Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary. Electronic address: konya@chem.u-szeged.hu.
Kolbert Z
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: kolzsu@bio.u-szeged.hu.

MeSH

BrassicaBrassica napusCell WallHydrogen PeroxideMustard PlantNanoparticlesNitric OxideOxidation-ReductionPlant RootsReactive Nitrogen SpeciesReactive Oxygen SpeciesSeedlingsSignal TransductionZinc Oxide

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32866892

Citation

Molnár, Árpád, et al. "ZnO Nanoparticles Induce Cell Wall Remodeling and Modify ROS/ RNS Signalling in Roots of Brassica Seedlings." Ecotoxicology and Environmental Safety, vol. 206, 2020, p. 111158.
Molnár Á, Rónavári A, Bélteky P, et al. ZnO nanoparticles induce cell wall remodeling and modify ROS/ RNS signalling in roots of Brassica seedlings. Ecotoxicol Environ Saf. 2020;206:111158.
Molnár, Á., Rónavári, A., Bélteky, P., Szőllősi, R., Valyon, E., Oláh, D., Rázga, Z., Ördög, A., Kónya, Z., & Kolbert, Z. (2020). ZnO nanoparticles induce cell wall remodeling and modify ROS/ RNS signalling in roots of Brassica seedlings. Ecotoxicology and Environmental Safety, 206, 111158. https://doi.org/10.1016/j.ecoenv.2020.111158
Molnár Á, et al. ZnO Nanoparticles Induce Cell Wall Remodeling and Modify ROS/ RNS Signalling in Roots of Brassica Seedlings. Ecotoxicol Environ Saf. 2020 Dec 15;206:111158. PubMed PMID: 32866892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ZnO nanoparticles induce cell wall remodeling and modify ROS/ RNS signalling in roots of Brassica seedlings. AU - Molnár,Árpád, AU - Rónavári,Andrea, AU - Bélteky,Péter, AU - Szőllősi,Réka, AU - Valyon,Emil, AU - Oláh,Dóra, AU - Rázga,Zsolt, AU - Ördög,Attila, AU - Kónya,Zoltán, AU - Kolbert,Zsuzsanna, Y1 - 2020/08/28/ PY - 2020/05/06/received PY - 2020/08/06/revised PY - 2020/08/08/accepted PY - 2020/9/1/pubmed PY - 2020/12/2/medline PY - 2020/9/1/entrez KW - Brassica juncea KW - Brassica napus KW - Cell wall modifications KW - Nitro-oxidative signalling KW - Zinc oxide nanoparticles SP - 111158 EP - 111158 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 206 N2 - Cell wall-associated defence against zinc oxide nanoparticles (ZnO NPs) as well as nitro-oxidative signalling and its consequences in plants are poorly examined. Therefore, this study compares the effect of chemically synthetized ZnO NPs (~45 nm, 25 or 100 mg/L) on Brassica napus and Brassica juncea seedlings. The effects on root biomass and viability suggest that B. napus is more tolerant to ZnO NP exposure relative to B. juncea. This may be due to the lack of Zn ion accumulation in the roots, which is related to the increase in the amount of lignin, suberin, pectin and in peroxidase activity in the roots of B. napus. TEM results indicate that root cell walls of 25 mg/L ZnO NP-treated B. napus may bind Zn ions. Additionally, callose accumulation possibly contribute to root shortening in both Brassica species as the effect of 100 mg/L ZnO NPs. Further results suggest that in the roots of the relatively sensitive B. juncea the levels of superoxide radical, hydrogen peroxide, hydrogen sulfide, nitric oxide, peroxinitrite and S-nitrosoglutathione increased as the effect of high ZnO NP concentration meaning that ZnO NP intensifies nitro-oxidative signalling. In B. napus; however, reactive oxygen species signalling was intensified, but reactive nitrogen species signalling wasn't activated by ZnO NPs. Collectively, these results indicate that ZnO NPs induce cell wall remodeling which may be associated with ZnO NP tolerance. Furthermore, plant tolerance against ZnO NPs is associated rather with nitrosative signalling than oxidative modifications. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/32866892/ZnO_nanoparticles_induce_cell_wall_remodeling_and_modify_ROS/_RNS_signalling_in_roots_of_Brassica_seedlings_ DB - PRIME DP - Unbound Medicine ER -