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UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis.
Biochem Biophys Res Commun. 2020 11 05; 532(2):244-250.BB

Abstract

Auxin is a key plant growth regulator for diverse developmental processes in plants. Indole-3-acetic acid (IAA) is a primary plant auxin that regulates the formation of various organs. Plants also produce phenylacetic acid (PAA), another natural auxin, which occurs more abundantly than IAA in various plant species. Although it has been demonstrated that the two auxins have distinct transport characteristics, the metabolic pathways and physiological roles of PAA in plants remain unsolved. In this study, we investigated the role of Arabidopsis UDP-glucosyltransferase UGT84B1 in IAA and PAA metabolism. We demonstrated that UGT84B1, which converts IAA to IAA-glucoside (IAA-Glc), can also catalyze the conversion of PAA to PAA-glucoside (PAA-Glc), with a higher catalytic activity in vitro. Furthermore, we showed a significant increase in both the IAA and PAA levels in the ugt84b1 null mutants. However, no obvious developmental phenotypes were observed in the ugt84b1 mutants under laboratory growth conditions. Moreover, the overexpression of UGT84B1 resulted in auxin-deficient root phenotypes and changes in the IAA and PAA levels. Our results indicate that UGT84B1 plays an important role in IAA and PAA homeostasis in Arabidopsis.

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Authors+Show Affiliations

Aoi Y
Department of Biological Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Hira H
Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Hayakawa Y
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Liu H
Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA.
Fukui K
Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan.
Dai X
Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA.
Tanaka K
Laboratory of Biochemistry, Wageningen University & Research, 6708 WE Wageningen, the Netherlands.
Hayashi KI
Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan.
Zhao Y
Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA.
Kasahara H
Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan; RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045, Japan. Electronic address: kasahara@go.tuat.ac.jp.

MeSH

ArabidopsisArabidopsis ProteinsCRISPR-Cas SystemsEscherichia coliGene Expression Regulation, PlantGlucosyltransferasesIndoleacetic AcidsMutationPhenylacetatesPlants, Genetically Modified

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32868079

Citation

Aoi, Yuki, et al. "UDP-glucosyltransferase UGT84B1 Regulates the Levels of Indole-3-acetic Acid and Phenylacetic Acid in Arabidopsis." Biochemical and Biophysical Research Communications, vol. 532, no. 2, 2020, pp. 244-250.
Aoi Y, Hira H, Hayakawa Y, et al. UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis. Biochem Biophys Res Commun. 2020;532(2):244-250.
Aoi, Y., Hira, H., Hayakawa, Y., Liu, H., Fukui, K., Dai, X., Tanaka, K., Hayashi, K. I., Zhao, Y., & Kasahara, H. (2020). UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis. Biochemical and Biophysical Research Communications, 532(2), 244-250. https://doi.org/10.1016/j.bbrc.2020.08.026
Aoi Y, et al. UDP-glucosyltransferase UGT84B1 Regulates the Levels of Indole-3-acetic Acid and Phenylacetic Acid in Arabidopsis. Biochem Biophys Res Commun. 2020 11 5;532(2):244-250. PubMed PMID: 32868079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis. AU - Aoi,Yuki, AU - Hira,Hayao, AU - Hayakawa,Yuya, AU - Liu,Hongquan, AU - Fukui,Kosuke, AU - Dai,Xinhua, AU - Tanaka,Keita, AU - Hayashi,Ken-Ichiro, AU - Zhao,Yunde, AU - Kasahara,Hiroyuki, Y1 - 2020/08/28/ PY - 2020/08/09/received PY - 2020/08/10/accepted PY - 2020/9/2/pubmed PY - 2021/3/19/medline PY - 2020/9/2/entrez KW - Auxin KW - Indole-3-acetic acid KW - Metabolism KW - Phenylacetic acid KW - UDP-Glucosyltransferase SP - 244 EP - 250 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 532 IS - 2 N2 - Auxin is a key plant growth regulator for diverse developmental processes in plants. Indole-3-acetic acid (IAA) is a primary plant auxin that regulates the formation of various organs. Plants also produce phenylacetic acid (PAA), another natural auxin, which occurs more abundantly than IAA in various plant species. Although it has been demonstrated that the two auxins have distinct transport characteristics, the metabolic pathways and physiological roles of PAA in plants remain unsolved. In this study, we investigated the role of Arabidopsis UDP-glucosyltransferase UGT84B1 in IAA and PAA metabolism. We demonstrated that UGT84B1, which converts IAA to IAA-glucoside (IAA-Glc), can also catalyze the conversion of PAA to PAA-glucoside (PAA-Glc), with a higher catalytic activity in vitro. Furthermore, we showed a significant increase in both the IAA and PAA levels in the ugt84b1 null mutants. However, no obvious developmental phenotypes were observed in the ugt84b1 mutants under laboratory growth conditions. Moreover, the overexpression of UGT84B1 resulted in auxin-deficient root phenotypes and changes in the IAA and PAA levels. Our results indicate that UGT84B1 plays an important role in IAA and PAA homeostasis in Arabidopsis. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/32868079/UDP_glucosyltransferase_UGT84B1_regulates_the_levels_of_indole_3_acetic_acid_and_phenylacetic_acid_in_Arabidopsis_ DB - PRIME DP - Unbound Medicine ER -