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[Effect of electroacupuncture of different acupoint groups on learning-memory ability and expression of IL-1β and TNF-α in hippocampus and prefrontal cortex in rats with Alzheimer's disease].
Zhen Ci Yan Jiu. 2020 Aug 25; 45(8):617-22.ZC

Abstract

OBJECTIVE

To compare the effect of electroacupuncture (EA) of acupoint group for "reinforcing the kidney and regulating Governor Vessel" and acopoint group for "reinforcing the kidney and lung and regulating Governor Vessel" on lear-ning-memory ability and expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) proteins in the hippocampus and prefrontal cortex (PFC) in Alzheimer's disease (AD) rats, so as to explore the efficacy of the two acupoint groups and mechanisms underlying improvement of AD.

METHODS

Forty male SD rats were randomly divided into control, sham operation, model, "Baihui" + "Shenshu" (GV20+BL23, for "reinforcing the kidney and regulating Governor Vessel") EA and GV20+BL23+ "Feishu" (BL13, GV20+BL23+BL13, for "reinforcing the kidney and lung and regulating Governor Vessel") EA groups (n=8 rats in each group). The AD model was established by bilateral injection of amyloid β peptide (Aβ25-35,10 μL) into bilateral hippocampus, and rats of the sham operation group received injection of normal saline. After successful establishment of the model,EA (2 Hz, 2 mA) was applied to these acupoints for 15 min, once daily for 10 days. Then, the learning-memory ability was assessed by using Morris water maze tests, and the expression levels of TNF-α and IL-1β proteins in the PFC and hippocampus tissues were detected by using Western blot.

RESULTS

Following modeling, the average escape latency of place navigation test were significantly increased (P<0.05) and the platform crossing times of spatial probe test was significantly decreased in the model group than in the control and sham operation groups (P<0.05). The expression levels of IL-1β and TNF-α proteins in the PFC and hippocampus were apparently up-regulated in the model group than in the control and the sham operation groups (P<0.000 1, P<0.001, P<0.01). After the intervention, the increase of the average escape latency and expression of IL-1β and TNF-α in the PFC and hippocampus, and the decrease of space exploration test were revised in both GV20+BL23 EA and GV20+BL23+BL13 EA groups (P<0.05,P<0.01). No significant differences were found between the GV20+BL23 and GV20+BL23+BL13 EA groups in the above mentioned indexes (P>0.05).

CONCLUSION

EA of both GV20+BL23 and GV20+BL23+BL13 acupoint can improve learning-memory ability of AD rats, which is associated with their effects in down-regulating the expression of IL-1β and TNF-α in the PFC and hippocampus to reduce inflammatory reaction. There were no significant differences between the two acupoint groups in the therapeutic effects.

Authors+Show Affiliations

Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.Department of Neurosurgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010.College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, 400016.College of Medicine, Chengdu University, Chengdu 610106.Department of Neurology, The Fifth People's Hospital of Chengdu, Chengdu 611130.Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.Department of Human Anatomy, Chongqing Medical University, Chongqing, 400016, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32869570

Citation

Wang, Yi-Ying, et al. "[Effect of Electroacupuncture of Different Acupoint Groups On Learning-memory Ability and Expression of IL-1β and TNF-α in Hippocampus and Prefrontal Cortex in Rats With Alzheimer's Disease]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 8, 2020, pp. 617-22.
Wang YY, Wu X, Tang CL, et al. [Effect of electroacupuncture of different acupoint groups on learning-memory ability and expression of IL-1β and TNF-α in hippocampus and prefrontal cortex in rats with Alzheimer's disease]. Zhen Ci Yan Jiu. 2020;45(8):617-22.
Wang, Y. Y., Wu, X., Tang, C. L., Xu, Y., Wang, J. R., Xu, J., Huang, J., Qiu, G. P., Sheng, H. J., & Zhu, S. J. (2020). [Effect of electroacupuncture of different acupoint groups on learning-memory ability and expression of IL-1β and TNF-α in hippocampus and prefrontal cortex in rats with Alzheimer's disease]. Zhen Ci Yan Jiu = Acupuncture Research, 45(8), 617-22. https://doi.org/10.13702/j.1000-0607.190887
Wang YY, et al. [Effect of Electroacupuncture of Different Acupoint Groups On Learning-memory Ability and Expression of IL-1β and TNF-α in Hippocampus and Prefrontal Cortex in Rats With Alzheimer's Disease]. Zhen Ci Yan Jiu. 2020 Aug 25;45(8):617-22. PubMed PMID: 32869570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture of different acupoint groups on learning-memory ability and expression of IL-1β and TNF-α in hippocampus and prefrontal cortex in rats with Alzheimer's disease]. AU - Wang,Yi-Ying, AU - Wu,Xuan, AU - Tang,Cheng-Lin, AU - Xu,Yi, AU - Wang,Jian-Rong, AU - Xu,Jin, AU - Huang,Juan, AU - Qiu,Guo-Ping, AU - Sheng,Hua-Jun, AU - Zhu,Shu-Juan, PY - 2020/9/2/entrez PY - 2020/9/2/pubmed PY - 2020/10/10/medline KW - Alzheimer’s disease KW - Electroacupuncture KW - Hippocampus KW - Interleukin-1β KW - Learning-memory ability KW - Prefrontal cortex KW - Tumor necrosis factor-α SP - 617 EP - 22 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 8 N2 - OBJECTIVE: To compare the effect of electroacupuncture (EA) of acupoint group for "reinforcing the kidney and regulating Governor Vessel" and acopoint group for "reinforcing the kidney and lung and regulating Governor Vessel" on lear-ning-memory ability and expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) proteins in the hippocampus and prefrontal cortex (PFC) in Alzheimer's disease (AD) rats, so as to explore the efficacy of the two acupoint groups and mechanisms underlying improvement of AD. METHODS: Forty male SD rats were randomly divided into control, sham operation, model, "Baihui" + "Shenshu" (GV20+BL23, for "reinforcing the kidney and regulating Governor Vessel") EA and GV20+BL23+ "Feishu" (BL13, GV20+BL23+BL13, for "reinforcing the kidney and lung and regulating Governor Vessel") EA groups (n=8 rats in each group). The AD model was established by bilateral injection of amyloid β peptide (Aβ25-35,10 μL) into bilateral hippocampus, and rats of the sham operation group received injection of normal saline. After successful establishment of the model,EA (2 Hz, 2 mA) was applied to these acupoints for 15 min, once daily for 10 days. Then, the learning-memory ability was assessed by using Morris water maze tests, and the expression levels of TNF-α and IL-1β proteins in the PFC and hippocampus tissues were detected by using Western blot. RESULTS: Following modeling, the average escape latency of place navigation test were significantly increased (P<0.05) and the platform crossing times of spatial probe test was significantly decreased in the model group than in the control and sham operation groups (P<0.05). The expression levels of IL-1β and TNF-α proteins in the PFC and hippocampus were apparently up-regulated in the model group than in the control and the sham operation groups (P<0.000 1, P<0.001, P<0.01). After the intervention, the increase of the average escape latency and expression of IL-1β and TNF-α in the PFC and hippocampus, and the decrease of space exploration test were revised in both GV20+BL23 EA and GV20+BL23+BL13 EA groups (P<0.05,P<0.01). No significant differences were found between the GV20+BL23 and GV20+BL23+BL13 EA groups in the above mentioned indexes (P>0.05). CONCLUSION: EA of both GV20+BL23 and GV20+BL23+BL13 acupoint can improve learning-memory ability of AD rats, which is associated with their effects in down-regulating the expression of IL-1β and TNF-α in the PFC and hippocampus to reduce inflammatory reaction. There were no significant differences between the two acupoint groups in the therapeutic effects. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32869570/[Effect_of_electroacupuncture_of_different_acupoint_groups_on_learning_memory_ability_and_expression_of_IL_1β_and_TNF_α_in_hippocampus_and_prefrontal_cortex_in_rats_with_Alzheimer's_disease]_ DB - PRIME DP - Unbound Medicine ER -