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Designing an efficient multi-epitope vaccine displaying interactions with diverse HLA molecules for an efficient humoral and cellular immune response to prevent COVID-19 infection.
Expert Rev Vaccines. 2020 09; 19(9):871-885.ER

Abstract

BACKGROUND

The novel SARS-CoV-2 coronavirus, the causative agent of the ongoing pandemic COVID-19 disease continues to infect people globally and has infected millions of humans worldwide. However, no effective vaccine against this virus exists.

METHOD

Using Immunoinformatics, epitopic sequences from multiple glycoproteins that play crucial role in pathogenesis were identified. Particularly, epitopes were mapped from conserved receptor-binding domain of spike protein which have been experimentally validated in SARS-CoV-1 as a promising target for vaccine development.

RESULTS

A multi-epitopic vaccine construct comprising of B-cell, CTL, HTL epitopes was developed along with fusion of adjuvant and linkers. The epitopes identified herein are reported for the first time and were predicted to be highly antigenic, stable, nonallergen, nontoxic and displayed conservation across several SARS-CoV-2 isolates from different countries. Additionally, the epitopes associated with maximum HLA alleles and population coverage analysis shows the proposed epitopes would be a relevant representative of large proportion of the world population. A reliable three-dimensional structure of the vaccine construct was developed. Consequently, docking and molecular-dynamics simulation ensured the stable interaction between vaccine and innate-immune receptor.

Authors+Show Affiliations

School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.Kalinga Institute of Medical Sciences (KIMS) Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India. KIIT-Technology Business Incubator (KIIT-TBI), Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India. KIIT-Technology Business Incubator (KIIT-TBI), Kalinga Institute of Industrial Technology (KIIT-DU) , Bhubaneswar 751024, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32869699

Citation

Mahapatra, Soumya Ranjan, et al. "Designing an Efficient Multi-epitope Vaccine Displaying Interactions With Diverse HLA Molecules for an Efficient Humoral and Cellular Immune Response to Prevent COVID-19 Infection." Expert Review of Vaccines, vol. 19, no. 9, 2020, pp. 871-885.
Mahapatra SR, Sahoo S, Dehury B, et al. Designing an efficient multi-epitope vaccine displaying interactions with diverse HLA molecules for an efficient humoral and cellular immune response to prevent COVID-19 infection. Expert Rev Vaccines. 2020;19(9):871-885.
Mahapatra, S. R., Sahoo, S., Dehury, B., Raina, V., Patro, S., Misra, N., & Suar, M. (2020). Designing an efficient multi-epitope vaccine displaying interactions with diverse HLA molecules for an efficient humoral and cellular immune response to prevent COVID-19 infection. Expert Review of Vaccines, 19(9), 871-885. https://doi.org/10.1080/14760584.2020.1811091
Mahapatra SR, et al. Designing an Efficient Multi-epitope Vaccine Displaying Interactions With Diverse HLA Molecules for an Efficient Humoral and Cellular Immune Response to Prevent COVID-19 Infection. Expert Rev Vaccines. 2020;19(9):871-885. PubMed PMID: 32869699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Designing an efficient multi-epitope vaccine displaying interactions with diverse HLA molecules for an efficient humoral and cellular immune response to prevent COVID-19 infection. AU - Mahapatra,Soumya Ranjan, AU - Sahoo,Susrita, AU - Dehury,Budheswar, AU - Raina,Vishakha, AU - Patro,Shubhransu, AU - Misra,Namrata, AU - Suar,Mrutyunjay, Y1 - 2020/09/24/ PY - 2020/9/2/pubmed PY - 2020/11/28/medline PY - 2020/9/2/entrez KW - COVID-19 KW - SARS-CoV-2 KW - immunoinformatics KW - multi-epitopic vaccine KW - receptor-binding domain SP - 871 EP - 885 JF - Expert review of vaccines JO - Expert Rev Vaccines VL - 19 IS - 9 N2 - BACKGROUND: The novel SARS-CoV-2 coronavirus, the causative agent of the ongoing pandemic COVID-19 disease continues to infect people globally and has infected millions of humans worldwide. However, no effective vaccine against this virus exists. METHOD: Using Immunoinformatics, epitopic sequences from multiple glycoproteins that play crucial role in pathogenesis were identified. Particularly, epitopes were mapped from conserved receptor-binding domain of spike protein which have been experimentally validated in SARS-CoV-1 as a promising target for vaccine development. RESULTS: A multi-epitopic vaccine construct comprising of B-cell, CTL, HTL epitopes was developed along with fusion of adjuvant and linkers. The epitopes identified herein are reported for the first time and were predicted to be highly antigenic, stable, nonallergen, nontoxic and displayed conservation across several SARS-CoV-2 isolates from different countries. Additionally, the epitopes associated with maximum HLA alleles and population coverage analysis shows the proposed epitopes would be a relevant representative of large proportion of the world population. A reliable three-dimensional structure of the vaccine construct was developed. Consequently, docking and molecular-dynamics simulation ensured the stable interaction between vaccine and innate-immune receptor. SN - 1744-8395 UR - https://www.unboundmedicine.com/medline/citation/32869699/Designing_an_efficient_multi_epitope_vaccine_displaying_interactions_with_diverse_HLA_molecules_for_an_efficient_humoral_and_cellular_immune_response_to_prevent_COVID_19_infection_ L2 - https://www.tandfonline.com/doi/full/10.1080/14760584.2020.1811091 DB - PRIME DP - Unbound Medicine ER -