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A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat.
Theor Appl Genet. 2020 Dec; 133(12):3381-3393.TA

Abstract

KEY MESSAGE

A novel, stably expressed, and plant height-independent QTL for spike extension length on 5AS was identified and validated in different populations using a newly developed and tightly linked KASP marker. As an important component of plant height (PH), spike extension length (SEL) plays a significant role in formation of an ideotype in wheat. Despite the fact that numerous loci for SEL in wheat have been reported, our knowledge on PH-independent loci remains to be limited. In this study, two recombinant inbred line (RIL) populations genotyped using the Wheat55K SNP were used to detect quantitative trait loci (QTL) controlling SEL across six environments. A total of 30 QTL for SEL were detected in these two RIL populations, and four of them, i.e., QSEL.sicau-2CN-4D, QSEL.sicau-2SY-4B.2, QSEL.sicau-2SY-4D.1, and QSEL.sicau-2CN-5A, were stably expressed. Genetic and conditional QTL analysis showed that the first three were significantly associated with PH, while the last one, QSEL.sicau-2CN-5A, is independent of PH. Comparison of genetic and physical maps suggested that only QSEL.sicau-2CN-5A located on chromosome arm 5AS is likely a novel QTL. A Kompetitive Allele-Specific PCR (KASP) marker, KASP-AX-110413733, tightly linked to this novel QTL was developed to successfully confirm its effect in three different genetic populations. Further, in the interval where QSEL.sicau-2CN-5A was located on 'Chinese Spring' wheat reference genome, three promising genes mainly expressed in wheat stem were predicated and they all encode the cytochrome P450 that was demonstrated to be closely associated with SEL elongation in rice. In addition, significant correlations between SEL and PH, spikelet number per spike, and thousand-grain weight were also detected. Altogether, our results broaden our understanding on genetic basis of SEL and will be useful for marker-based selection of lines with different SELs and fine mapping the novel and PH-independent QTL QSEL.sicau-2CN-5A.

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Authors+Show Affiliations

Li C
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Tang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Luo W
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Zhang X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Mu Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Deng M
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Liu Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Jiang Q
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Chen G
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Wang J
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Qi P
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Pu Z
College of Agronomy, Sichuan Agricultural University, Chengdu, China.
Jiang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Wei Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Zheng Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Lan X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. lanxiujin@163.com.
Ma J
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. jianma@sicau.edu.cn.

MeSH

Chromosome MappingChromosomes, PlantGenetic MarkersGenetics, PopulationGenotypePhenotypePlant BreedingPolymorphism, Single NucleotideQuantitative Trait LociSeedsTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32870326

Citation

Li, Cong, et al. "A Novel, Validated, and Plant Height-independent QTL for Spike Extension Length Is Associated With Yield-related Traits in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 12, 2020, pp. 3381-3393.
Li C, Tang H, Luo W, et al. A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. Theor Appl Genet. 2020;133(12):3381-3393.
Li, C., Tang, H., Luo, W., Zhang, X., Mu, Y., Deng, M., Liu, Y., Jiang, Q., Chen, G., Wang, J., Qi, P., Pu, Z., Jiang, Y., Wei, Y., Zheng, Y., Lan, X., & Ma, J. (2020). A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(12), 3381-3393. https://doi.org/10.1007/s00122-020-03675-0
Li C, et al. A Novel, Validated, and Plant Height-independent QTL for Spike Extension Length Is Associated With Yield-related Traits in Wheat. Theor Appl Genet. 2020;133(12):3381-3393. PubMed PMID: 32870326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. AU - Li,Cong, AU - Tang,Huaping, AU - Luo,Wei, AU - Zhang,Xuemei, AU - Mu,Yang, AU - Deng,Mei, AU - Liu,Yaxi, AU - Jiang,Qiantao, AU - Chen,Guoyue, AU - Wang,Jirui, AU - Qi,Pengfei, AU - Pu,Zhien, AU - Jiang,Yunfeng, AU - Wei,Yuming, AU - Zheng,Youliang, AU - Lan,Xiujin, AU - Ma,Jian, Y1 - 2020/09/01/ PY - 2020/06/15/received PY - 2020/08/21/accepted PY - 2020/9/2/pubmed PY - 2021/5/29/medline PY - 2020/9/2/entrez SP - 3381 EP - 3393 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 12 N2 - KEY MESSAGE: A novel, stably expressed, and plant height-independent QTL for spike extension length on 5AS was identified and validated in different populations using a newly developed and tightly linked KASP marker. As an important component of plant height (PH), spike extension length (SEL) plays a significant role in formation of an ideotype in wheat. Despite the fact that numerous loci for SEL in wheat have been reported, our knowledge on PH-independent loci remains to be limited. In this study, two recombinant inbred line (RIL) populations genotyped using the Wheat55K SNP were used to detect quantitative trait loci (QTL) controlling SEL across six environments. A total of 30 QTL for SEL were detected in these two RIL populations, and four of them, i.e., QSEL.sicau-2CN-4D, QSEL.sicau-2SY-4B.2, QSEL.sicau-2SY-4D.1, and QSEL.sicau-2CN-5A, were stably expressed. Genetic and conditional QTL analysis showed that the first three were significantly associated with PH, while the last one, QSEL.sicau-2CN-5A, is independent of PH. Comparison of genetic and physical maps suggested that only QSEL.sicau-2CN-5A located on chromosome arm 5AS is likely a novel QTL. A Kompetitive Allele-Specific PCR (KASP) marker, KASP-AX-110413733, tightly linked to this novel QTL was developed to successfully confirm its effect in three different genetic populations. Further, in the interval where QSEL.sicau-2CN-5A was located on 'Chinese Spring' wheat reference genome, three promising genes mainly expressed in wheat stem were predicated and they all encode the cytochrome P450 that was demonstrated to be closely associated with SEL elongation in rice. In addition, significant correlations between SEL and PH, spikelet number per spike, and thousand-grain weight were also detected. Altogether, our results broaden our understanding on genetic basis of SEL and will be useful for marker-based selection of lines with different SELs and fine mapping the novel and PH-independent QTL QSEL.sicau-2CN-5A. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/32870326/A_novel_validated_and_plant_height_independent_QTL_for_spike_extension_length_is_associated_with_yield_related_traits_in_wheat_ L2 - https://dx.doi.org/10.1007/s00122-020-03675-0 DB - PRIME DP - Unbound Medicine ER -