Tags

Type your tag names separated by a space and hit enter

DNA damage and oxygen radical toxicity.
Science 1988; 240(4857):1302-9Sci

Abstract

A major portion of the toxicity of hydrogen peroxide in Escherichia coli is attributed to DNA damage mediated by a Fenton reaction that generates active forms of hydroxyl radicals from hydrogen peroxide, DNA-bound iron, and a constant source of reducing equivalents. Kinetic peculiarities of DNA damage production by hydrogen peroxide in vivo can be reproduced by including DNA in an in vitro Fenton reaction system in which iron catalyzes the univalent reduction of hydrogen peroxide by the reduced form of nicotinamide adenine dinucleotide (NADH). To minimize the toxicity of oxygen radicals, the cell utilizes scavengers of these radicals and DNA repair enzymes. On the basis of observations with the model system, it is proposed that the cell may also decrease such toxicity by diminishing available NAD(P)H and by utilizing oxygen itself to scavenge active free radicals into superoxide, which is then destroyed by superoxide dismutase.

Authors+Show Affiliations

University of California, Berkeley.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

3287616

Citation

Imlay, J A., and S Linn. "DNA Damage and Oxygen Radical Toxicity." Science (New York, N.Y.), vol. 240, no. 4857, 1988, pp. 1302-9.
Imlay JA, Linn S. DNA damage and oxygen radical toxicity. Science. 1988;240(4857):1302-9.
Imlay, J. A., & Linn, S. (1988). DNA damage and oxygen radical toxicity. Science (New York, N.Y.), 240(4857), pp. 1302-9.
Imlay JA, Linn S. DNA Damage and Oxygen Radical Toxicity. Science. 1988 Jun 3;240(4857):1302-9. PubMed PMID: 3287616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA damage and oxygen radical toxicity. AU - Imlay,J A, AU - Linn,S, PY - 1988/6/3/pubmed PY - 1988/6/3/medline PY - 1988/6/3/entrez SP - 1302 EP - 9 JF - Science (New York, N.Y.) JO - Science VL - 240 IS - 4857 N2 - A major portion of the toxicity of hydrogen peroxide in Escherichia coli is attributed to DNA damage mediated by a Fenton reaction that generates active forms of hydroxyl radicals from hydrogen peroxide, DNA-bound iron, and a constant source of reducing equivalents. Kinetic peculiarities of DNA damage production by hydrogen peroxide in vivo can be reproduced by including DNA in an in vitro Fenton reaction system in which iron catalyzes the univalent reduction of hydrogen peroxide by the reduced form of nicotinamide adenine dinucleotide (NADH). To minimize the toxicity of oxygen radicals, the cell utilizes scavengers of these radicals and DNA repair enzymes. On the basis of observations with the model system, it is proposed that the cell may also decrease such toxicity by diminishing available NAD(P)H and by utilizing oxygen itself to scavenge active free radicals into superoxide, which is then destroyed by superoxide dismutase. SN - 0036-8075 UR - https://www.unboundmedicine.com/medline/citation/3287616/DNA_damage_and_oxygen_radical_toxicity_ L2 - http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=3287616 DB - PRIME DP - Unbound Medicine ER -