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Serum macrophage migration inhibition factor for diagnosing endometriosis and its severity: case-control study.
BMC Womens Health. 2020 09 03; 20(1):189.BW

Abstract

BACKGROUND

Endometriosis is a long-standing progressive disease that affects women of reproductive age. Macrophage migration inhibitory factor (MIF) is one of non-invasive blood biomarker that was detected in sera of endometriotic patients. The present study aimed to determine the accuracy of serum MIF in diagnosing endometriosis in women with infertility and chronic pelvic pain, and correlate its level to the stage of the disease.

METHODS

Observational case-control study conducted at Fayoum University hospital from March 2016 till September 2018. Three hundred women candidate for diagnostic laparoscopy for either infertility or gynecologic chronic pelvic pain were included. The study group included patients with symptoms suggestive of endometriosis or chocolate cyst by ultrasound and proved by laparoscopy and histopathology. The control group included other causes of infertility or pelvic pain. All patients undergone either diagnostic or operative laparoscopy, and before laparoscopy blood sampling for quantitative measurement of macrophage migration inhibitory factor (MIF) protein in serum by ELISA technique.

RESULTS

The level of serum MIF was significantly higher in endometriosis group compared to control group (1.75 ± 1.48 pg/ml and 0.51 ± 0.45 pg/ ml, respectively, P = < 0.001), with a progressive increase with advancing stage (stage I, 1.3 ± 1.03 pg/ml, stage II, 1.7 ± 1.57 pg/ml, stage III, 2.1 ± 1.19 pg/ml and in stage IV, 3.2 ± 2.6 pg/ml). Moreover, in patients presented with pain and infertile patients showed significantly higher levels of serum MIF (1.92 ± 1.13 vs 1.21 ± 1.17 and 1.82 ± 1.13 vs 1.32 ± 0.91 respectively with p-value < 0.001). ROC curve of serum MIF with a cut off value of 0.85 pg/ml or more achieves a sensitivity of 80.6%, specificity of 83.3%, positive predictive value of 82.9% and negative predictive value of 81.2%.

CONCLUSION

Serum MIF might be a promising marker not only for noninvasive diagnosis of endometriosis but as a target for detecting severity as well.

Authors+Show Affiliations

Obstetric and Gynecology Department, Fayoum University, 23 Mohammed Gonemy of mohammed Elmakreef 6th district, nasr city, Cairo, Fayoum, Egypt. sbaradie@gmail.com.Obstetric and Gynecology Department, Fayoum University, 23 Mohammed Gonemy of mohammed Elmakreef 6th district, nasr city, Cairo, Fayoum, Egypt.Obstetric and Gynecology Department, Fayoum University, 23 Mohammed Gonemy of mohammed Elmakreef 6th district, nasr city, Cairo, Fayoum, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32883256

Citation

Elbaradie, Sahar Mohamed Yehya, et al. "Serum Macrophage Migration Inhibition Factor for Diagnosing Endometriosis and Its Severity: Case-control Study." BMC Women's Health, vol. 20, no. 1, 2020, p. 189.
Elbaradie SMY, Bakry MS, Bosilah AH. Serum macrophage migration inhibition factor for diagnosing endometriosis and its severity: case-control study. BMC Womens Health. 2020;20(1):189.
Elbaradie, S. M. Y., Bakry, M. S., & Bosilah, A. H. (2020). Serum macrophage migration inhibition factor for diagnosing endometriosis and its severity: case-control study. BMC Women's Health, 20(1), 189. https://doi.org/10.1186/s12905-020-01051-0
Elbaradie SMY, Bakry MS, Bosilah AH. Serum Macrophage Migration Inhibition Factor for Diagnosing Endometriosis and Its Severity: Case-control Study. BMC Womens Health. 2020 09 3;20(1):189. PubMed PMID: 32883256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum macrophage migration inhibition factor for diagnosing endometriosis and its severity: case-control study. AU - Elbaradie,Sahar Mohamed Yehya, AU - Bakry,Mohamed Sobhy, AU - Bosilah,Almandouh Hussein, Y1 - 2020/09/03/ PY - 2020/02/21/received PY - 2020/08/16/accepted PY - 2020/9/5/entrez PY - 2020/9/5/pubmed PY - 2021/2/12/medline KW - Chronic pelvic pain KW - Diagnosis KW - Endometriosis KW - Infertility KW - Laparoscopy KW - Serum macrophage migration inhibition factor SP - 189 EP - 189 JF - BMC women's health JO - BMC Womens Health VL - 20 IS - 1 N2 - BACKGROUND: Endometriosis is a long-standing progressive disease that affects women of reproductive age. Macrophage migration inhibitory factor (MIF) is one of non-invasive blood biomarker that was detected in sera of endometriotic patients. The present study aimed to determine the accuracy of serum MIF in diagnosing endometriosis in women with infertility and chronic pelvic pain, and correlate its level to the stage of the disease. METHODS: Observational case-control study conducted at Fayoum University hospital from March 2016 till September 2018. Three hundred women candidate for diagnostic laparoscopy for either infertility or gynecologic chronic pelvic pain were included. The study group included patients with symptoms suggestive of endometriosis or chocolate cyst by ultrasound and proved by laparoscopy and histopathology. The control group included other causes of infertility or pelvic pain. All patients undergone either diagnostic or operative laparoscopy, and before laparoscopy blood sampling for quantitative measurement of macrophage migration inhibitory factor (MIF) protein in serum by ELISA technique. RESULTS: The level of serum MIF was significantly higher in endometriosis group compared to control group (1.75 ± 1.48 pg/ml and 0.51 ± 0.45 pg/ ml, respectively, P = < 0.001), with a progressive increase with advancing stage (stage I, 1.3 ± 1.03 pg/ml, stage II, 1.7 ± 1.57 pg/ml, stage III, 2.1 ± 1.19 pg/ml and in stage IV, 3.2 ± 2.6 pg/ml). Moreover, in patients presented with pain and infertile patients showed significantly higher levels of serum MIF (1.92 ± 1.13 vs 1.21 ± 1.17 and 1.82 ± 1.13 vs 1.32 ± 0.91 respectively with p-value < 0.001). ROC curve of serum MIF with a cut off value of 0.85 pg/ml or more achieves a sensitivity of 80.6%, specificity of 83.3%, positive predictive value of 82.9% and negative predictive value of 81.2%. CONCLUSION: Serum MIF might be a promising marker not only for noninvasive diagnosis of endometriosis but as a target for detecting severity as well. SN - 1472-6874 UR - https://www.unboundmedicine.com/medline/citation/32883256/Serum_macrophage_migration_inhibition_factor_for_diagnosing_endometriosis_and_its_severity:_case_control_study_ L2 - https://bmcwomenshealth.biomedcentral.com/articles/10.1186/s12905-020-01051-0 DB - PRIME DP - Unbound Medicine ER -