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The use of germicidal ultraviolet light, vaporized hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with a SARS-CoV-2 surrogate virus.
J Hosp Infect. 2020 Nov; 106(3):577-584.JH

Abstract

BACKGROUND

In the context of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, the supply of personal protective equipment remains under severe strain. To address this issue, re-use of surgical face masks and filtering facepiece respirators has been recommended; prior decontamination is paramount to their re-use.

AIM

We aim to provide information on the effects of three decontamination procedures on porcine respiratory coronavirus (PRCV)-contaminated masks and respirators, presenting a stable model for infectious coronavirus decontamination of these typically single-use-only products.

METHODS

Surgical masks and filtering facepiece respirator coupons and straps were inoculated with infectious PRCV and submitted to three decontamination treatments, ultraviolet (UV) irradiation, vaporized H2O2, and dry heat treatment. Viruses were recovered from sample materials and viral titres were measured in swine testicle cells.

FINDINGS

UV irradiation, vaporized H2O2 and dry heat reduced infectious PRCV by more than three orders of magnitude on mask and respirator coupons and rendered it undetectable in all decontamination assays.

CONCLUSION

This is the first description of stable disinfection of face masks and filtering facepiece respirators contaminated with an infectious SARS-CoV-2 surrogate using UV irradiation, vaporized H2O2 and dry heat treatment. The three methods permit demonstration of a loss of infectivity by more than three orders of magnitude of an infectious coronavirus in line with the United States Food and Drug Administration policy regarding face masks and respirators. It presents advantages of uncomplicated manipulation and utilization in a BSL2 facility, therefore being easily adaptable to other respirator and mask types.

Authors+Show Affiliations

Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, FARAH Research Centre, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, FARAH Research Centre, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, FARAH Research Centre, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.Department of Hospital Pharmacy, The University Hospital Center, University of Liège, Liège, Belgium.Department of Hospital Pharmacy, The University Hospital Center, University of Liège, Liège, Belgium.Centexbel Textile Research Centre, Grace-Hollogne, Belgium.TERRA Research Centre, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, FARAH Research Centre, Faculty of Veterinary Medicine, Liège University, Liège, Belgium. Electronic address: etienne.thiry@uliege.be.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32889029

Citation

Ludwig-Begall, L F., et al. "The Use of Germicidal Ultraviolet Light, Vaporized Hydrogen Peroxide and Dry Heat to Decontaminate Face Masks and Filtering Respirators Contaminated With a SARS-CoV-2 Surrogate Virus." The Journal of Hospital Infection, vol. 106, no. 3, 2020, pp. 577-584.
Ludwig-Begall LF, Wielick C, Dams L, et al. The use of germicidal ultraviolet light, vaporized hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with a SARS-CoV-2 surrogate virus. J Hosp Infect. 2020;106(3):577-584.
Ludwig-Begall, L. F., Wielick, C., Dams, L., Nauwynck, H., Demeuldre, P. F., Napp, A., Laperre, J., Haubruge, E., & Thiry, E. (2020). The use of germicidal ultraviolet light, vaporized hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with a SARS-CoV-2 surrogate virus. The Journal of Hospital Infection, 106(3), 577-584. https://doi.org/10.1016/j.jhin.2020.08.025
Ludwig-Begall LF, et al. The Use of Germicidal Ultraviolet Light, Vaporized Hydrogen Peroxide and Dry Heat to Decontaminate Face Masks and Filtering Respirators Contaminated With a SARS-CoV-2 Surrogate Virus. J Hosp Infect. 2020;106(3):577-584. PubMed PMID: 32889029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of germicidal ultraviolet light, vaporized hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with a SARS-CoV-2 surrogate virus. AU - Ludwig-Begall,L F, AU - Wielick,C, AU - Dams,L, AU - Nauwynck,H, AU - Demeuldre,P-F, AU - Napp,A, AU - Laperre,J, AU - Haubruge,E, AU - Thiry,E, Y1 - 2020/09/01/ PY - 2020/05/27/received PY - 2020/08/26/accepted PY - 2020/9/6/pubmed PY - 2020/11/24/medline PY - 2020/9/5/entrez KW - Decontamination (UV, H(2)O(2), dry heat) KW - PRCV KW - Respirator KW - SARS-CoV-2 KW - Surgical mask SP - 577 EP - 584 JF - The Journal of hospital infection JO - J Hosp Infect VL - 106 IS - 3 N2 - BACKGROUND: In the context of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, the supply of personal protective equipment remains under severe strain. To address this issue, re-use of surgical face masks and filtering facepiece respirators has been recommended; prior decontamination is paramount to their re-use. AIM: We aim to provide information on the effects of three decontamination procedures on porcine respiratory coronavirus (PRCV)-contaminated masks and respirators, presenting a stable model for infectious coronavirus decontamination of these typically single-use-only products. METHODS: Surgical masks and filtering facepiece respirator coupons and straps were inoculated with infectious PRCV and submitted to three decontamination treatments, ultraviolet (UV) irradiation, vaporized H2O2, and dry heat treatment. Viruses were recovered from sample materials and viral titres were measured in swine testicle cells. FINDINGS: UV irradiation, vaporized H2O2 and dry heat reduced infectious PRCV by more than three orders of magnitude on mask and respirator coupons and rendered it undetectable in all decontamination assays. CONCLUSION: This is the first description of stable disinfection of face masks and filtering facepiece respirators contaminated with an infectious SARS-CoV-2 surrogate using UV irradiation, vaporized H2O2 and dry heat treatment. The three methods permit demonstration of a loss of infectivity by more than three orders of magnitude of an infectious coronavirus in line with the United States Food and Drug Administration policy regarding face masks and respirators. It presents advantages of uncomplicated manipulation and utilization in a BSL2 facility, therefore being easily adaptable to other respirator and mask types. SN - 1532-2939 UR - https://www.unboundmedicine.com/medline/citation/32889029/The_use_of_germicidal_ultraviolet_light_vaporized_hydrogen_peroxide_and_dry_heat_to_decontaminate_face_masks_and_filtering_respirators_contaminated_with_a_SARS_CoV_2_surrogate_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0195-6701(20)30413-8 DB - PRIME DP - Unbound Medicine ER -