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Leaflet initiation and blade expansion are separable in compound leaf development.
Plant J. 2020 11; 104(4):1073-1087.PJ

Abstract

Compound leaves are composed of multiple separate blade units termed leaflets. In tomato (Solanum lycopersicum) compound leaves, auxin promotes both leaflet initiation and blade expansion. However, it is unclear how these two developmental processes interact. With highly variable complexity, tomato compound leaves provide an ideal system to address this question. In this study, we obtained and analyzed mutants of the WUSCHEL-RELATED HOMEOBOX (WOX) family gene SlLAM1 from tomato, whose orthologs in tobacco (Nicotiana sylvestris) and other species are indispensable for blade expansion. We show that SlLAM1 is expressed in the middle and marginal domains of leaves, and is required for blade expansion in leaflets. We demonstrate that sllam1 mutants cause a delay of leaflet initiation and slightly alter the arrangement of first-order leaflets, whereas the overall leaflet number is comparable to that of wild-type leaves. Analysis of the genetic interactions between SlLAM1 and key auxin signaling components revealed an epistatic effect of SlLAM1 in determining the final leaf form. Finally, we show that SlLAM1 is also required for floral organ growth and affects the fertility of gametophytes. Our data suggest that SlLAM1 promotes blade expansion in multiple leaf types, and leaflet initiation can be largely uncoupled from blade expansion during compound leaf morphogenesis.

Authors+Show Affiliations

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, PO Box 12, Rehovot, 76100, Israel.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel.The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, PO Box 12, Rehovot, 76100, Israel.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32889762

Citation

Du, Fei, et al. "Leaflet Initiation and Blade Expansion Are Separable in Compound Leaf Development." The Plant Journal : for Cell and Molecular Biology, vol. 104, no. 4, 2020, pp. 1073-1087.
Du F, Mo Y, Israeli A, et al. Leaflet initiation and blade expansion are separable in compound leaf development. Plant J. 2020;104(4):1073-1087.
Du, F., Mo, Y., Israeli, A., Wang, Q., Yifhar, T., Ori, N., & Jiao, Y. (2020). Leaflet initiation and blade expansion are separable in compound leaf development. The Plant Journal : for Cell and Molecular Biology, 104(4), 1073-1087. https://doi.org/10.1111/tpj.14982
Du F, et al. Leaflet Initiation and Blade Expansion Are Separable in Compound Leaf Development. Plant J. 2020;104(4):1073-1087. PubMed PMID: 32889762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leaflet initiation and blade expansion are separable in compound leaf development. AU - Du,Fei, AU - Mo,Yajin, AU - Israeli,Alon, AU - Wang,Qingqing, AU - Yifhar,Tamar, AU - Ori,Naomi, AU - Jiao,Yuling, Y1 - 2020/09/25/ PY - 2020/01/15/received PY - 2020/08/12/revised PY - 2020/08/17/accepted PY - 2020/9/6/pubmed PY - 2021/5/11/medline PY - 2020/9/5/entrez KW - Solanum lycopersicum KW - SlLAM1 KW - SlWOX1 KW - auxin KW - blastozone KW - compound leaf KW - flower development KW - leaf meristem KW - tomato SP - 1073 EP - 1087 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 104 IS - 4 N2 - Compound leaves are composed of multiple separate blade units termed leaflets. In tomato (Solanum lycopersicum) compound leaves, auxin promotes both leaflet initiation and blade expansion. However, it is unclear how these two developmental processes interact. With highly variable complexity, tomato compound leaves provide an ideal system to address this question. In this study, we obtained and analyzed mutants of the WUSCHEL-RELATED HOMEOBOX (WOX) family gene SlLAM1 from tomato, whose orthologs in tobacco (Nicotiana sylvestris) and other species are indispensable for blade expansion. We show that SlLAM1 is expressed in the middle and marginal domains of leaves, and is required for blade expansion in leaflets. We demonstrate that sllam1 mutants cause a delay of leaflet initiation and slightly alter the arrangement of first-order leaflets, whereas the overall leaflet number is comparable to that of wild-type leaves. Analysis of the genetic interactions between SlLAM1 and key auxin signaling components revealed an epistatic effect of SlLAM1 in determining the final leaf form. Finally, we show that SlLAM1 is also required for floral organ growth and affects the fertility of gametophytes. Our data suggest that SlLAM1 promotes blade expansion in multiple leaf types, and leaflet initiation can be largely uncoupled from blade expansion during compound leaf morphogenesis. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/32889762/Leaflet_initiation_and_blade_expansion_are_separable_in_compound_leaf_development_ L2 - https://doi.org/10.1111/tpj.14982 DB - PRIME DP - Unbound Medicine ER -