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The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2.
Int J Infect Dis. 2020 Sep 03; 100:224-229.IJ

Abstract

OBJECTIVES

There are currently no studies that have examined whether one dosage can be uniformly applied to different respirator types to effectively decontaminate SARS-CoV-2 on N95 filtering facepiece respirators (FFRs). Health care workers have been using this disinfection method during the pandemic. Our objective was to determine the effect of UVC on SARS-CoV-2 inoculated N95 respirators and whether this was respirator material/model type dependent.

METHODS

Four different locations (facepiece and strap) on five different N95 FFR models (3M 1860, 8210, 8511, 9211; Moldex 1511) were inoculated with a 10 μL drop of SARS-CoV-2 viral stock (8 × 107 TCID50/mL). The outside-facing and wearer-facing surfaces of the respirators were each irradiated with a dose of 1.5 J/cm2 UVC (254 nm). Viable SARS-CoV-2 was quantified by a median tissue culture infectious dose assay (TCID50).

RESULTS

UVC delivered using a dose of 1.5 J/cm2, to each side, was an effective method of decontamination for the facepieces of 3M 1860 and Moldex 1511, and for the straps of 3M 8210 and the Moldex 1511.

CONCLUSION

This dose is an appropriate decontamination method to facilitate the reuse of respirators for healthcare personnel when applied to specific models/materials. Also, some straps may require additional disinfection to maximize the safety of frontline workers. Implementation of widespread UVC decontamination methods requires careful consideration of model, material type, design, and fit-testing following irradiation.

Authors+Show Affiliations

Photomedicine and Photobiology Unit, Department of Dermatology, Henry Ford Health System, Detroit, MI 48202, USA. Electronic address: DOZOG1@hfhs.org.Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Medicinal Chemistry, College of Pharmacy, Ann Arbor, MI 48109, USA; University of Michigan Center for Drug Repurposing, USA.Photomedicine and Photobiology Unit, Department of Dermatology, Henry Ford Health System, Detroit, MI 48202, USA.Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.Photomedicine and Photobiology Unit, Department of Dermatology, Henry Ford Health System, Detroit, MI 48202, USA.Photomedicine and Photobiology Unit, Department of Dermatology, Henry Ford Health System, Detroit, MI 48202, USA.Department of Microbiology, Department of Pathology and Laboratory Medicine, Henry Ford Health System, Detroit, MI 48202, USA.Center for Cutaneous Biology and Immunology, Department of Dermatology, Henry Ford Health System, Detroit, MI 48202, USA; Immunology Program/Henry Ford Cancer Institute, Henry Ford Health System, Detroit, MI 48202, USA; Department of Internal Medicine, Henry Ford Health System, Detroit, MI 48202, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32891736

Citation

Ozog, David M., et al. "The Effect of Ultraviolet C Radiation Against Different N95 Respirators Inoculated With SARS-CoV-2." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 100, 2020, pp. 224-229.
Ozog DM, Sexton JZ, Narla S, et al. The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2. Int J Infect Dis. 2020;100:224-229.
Ozog, D. M., Sexton, J. Z., Narla, S., Pretto-Kernahan, C. D., Mirabelli, C., Lim, H. W., Hamzavi, I. H., Tibbetts, R. J., & Mi, Q. S. (2020). The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 100, 224-229. https://doi.org/10.1016/j.ijid.2020.08.077
Ozog DM, et al. The Effect of Ultraviolet C Radiation Against Different N95 Respirators Inoculated With SARS-CoV-2. Int J Infect Dis. 2020 Sep 3;100:224-229. PubMed PMID: 32891736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2. AU - Ozog,David M, AU - Sexton,Jonathan Z, AU - Narla,Shanthi, AU - Pretto-Kernahan,Carla D, AU - Mirabelli,Carmen, AU - Lim,Henry W, AU - Hamzavi,Iltefat H, AU - Tibbetts,Robert J, AU - Mi,Qing-Sheng, Y1 - 2020/09/03/ PY - 2020/07/19/received PY - 2020/08/24/revised PY - 2020/08/28/accepted PY - 2020/9/7/pubmed PY - 2020/9/7/medline PY - 2020/9/6/entrez KW - COVID-19 KW - Healthcare personnel KW - N95 KW - Reuse KW - SARS-Co-V2 KW - Ultraviolet C SP - 224 EP - 229 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 100 N2 - OBJECTIVES: There are currently no studies that have examined whether one dosage can be uniformly applied to different respirator types to effectively decontaminate SARS-CoV-2 on N95 filtering facepiece respirators (FFRs). Health care workers have been using this disinfection method during the pandemic. Our objective was to determine the effect of UVC on SARS-CoV-2 inoculated N95 respirators and whether this was respirator material/model type dependent. METHODS: Four different locations (facepiece and strap) on five different N95 FFR models (3M 1860, 8210, 8511, 9211; Moldex 1511) were inoculated with a 10 μL drop of SARS-CoV-2 viral stock (8 × 107 TCID50/mL). The outside-facing and wearer-facing surfaces of the respirators were each irradiated with a dose of 1.5 J/cm2 UVC (254 nm). Viable SARS-CoV-2 was quantified by a median tissue culture infectious dose assay (TCID50). RESULTS: UVC delivered using a dose of 1.5 J/cm2, to each side, was an effective method of decontamination for the facepieces of 3M 1860 and Moldex 1511, and for the straps of 3M 8210 and the Moldex 1511. CONCLUSION: This dose is an appropriate decontamination method to facilitate the reuse of respirators for healthcare personnel when applied to specific models/materials. Also, some straps may require additional disinfection to maximize the safety of frontline workers. Implementation of widespread UVC decontamination methods requires careful consideration of model, material type, design, and fit-testing following irradiation. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/32891736/The_Effect_of_Ultraviolet_C_Radiation_Against_Different_N95_Respirators_Inoculated_with_SARS_CoV_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(20)30709-8 DB - PRIME DP - Unbound Medicine ER -