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Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs.
Cornea. 2021 Mar 01; 40(3):348-350.C

Abstract

PURPOSE

This study was designed to detect CoV-RNA in the tears of polymerase chain reaction (PCR)-confirmed SARS-CoV-2 positive patients.

METHODS

We performed a prospective case series study of hospitalized patients who have been confirmed SARS-CoV-2 positive by oropharyngeal swab within the previous 5 days. Tear samples obtained with a laboratory capillary and oropharyngeal swabs were analyzed by real-time PCR using the Altona SARS-CoV-2 Assay or the Roche SARS-CoV-2 LightMix PCR, depending on the availability. Patient history was documented, and ophthalmoscopy was used to assess for ocular surface disease.

RESULTS

Of all 18 patients recruited in April 2020, 5 suffered from respiratory failure and were submitted to an intensive care unit. None of our patients had signs of viral conjunctivitis although all patients in intensive care showed chemosis and conjunctival hyperemia because of third-spacing or fluid overload. The presence of coronavirus RNA was confirmed by PCR in 5 of 18 patients (28%) in tears and 72% for oropharyngeal swabs.

CONCLUSIONS

Using a tear fluid sampling technique similar to oropharyngeal lavage presents a higher percentage of SARS-CoV-2 positive tears in contrast to earlier reports that used a conjunctival swab. This does not automatically indicate viral shedding in ocular tissue or contagiousness of tear fluid.

Authors+Show Affiliations

Aachen Centre of Technology Transfer in Ophthalmology (ACTO e.V.), An-Institute, RWTH Aachen University, Aachen, Germany.GenExpress Gesellschaft für Proteindesign mbH, Berlin, Germany; and.Institut für Pathologie der Kliniken der Stadt Köln, Klinikum der Privaten Universität Witten/Herdecke, Witten, Germany.Aachen Centre of Technology Transfer in Ophthalmology (ACTO e.V.), An-Institute, RWTH Aachen University, Aachen, Germany.Aachen Centre of Technology Transfer in Ophthalmology (ACTO e.V.), An-Institute, RWTH Aachen University, Aachen, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32897895

Citation

Dutescu, R Michael, et al. "Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs." Cornea, vol. 40, no. 3, 2021, pp. 348-350.
Dutescu RM, Banasik P, Schildgen O, et al. Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs. Cornea. 2021;40(3):348-350.
Dutescu, R. M., Banasik, P., Schildgen, O., Schrage, N., & Uthoff, D. (2021). Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs. Cornea, 40(3), 348-350. https://doi.org/10.1097/ICO.0000000000002562
Dutescu RM, et al. Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs. Cornea. 2021 Mar 1;40(3):348-350. PubMed PMID: 32897895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Coronavirus in Tear Samples of Hospitalized Patients With Confirmed SARS-CoV-2 From Oropharyngeal Swabs. AU - Dutescu,R Michael, AU - Banasik,Peter, AU - Schildgen,Oliver, AU - Schrage,Norbert, AU - Uthoff,Daniel, PY - 2020/06/05/received PY - 2020/08/29/accepted PY - 2020/9/9/pubmed PY - 2021/2/13/medline PY - 2020/9/8/entrez SP - 348 EP - 350 JF - Cornea JO - Cornea VL - 40 IS - 3 N2 - PURPOSE: This study was designed to detect CoV-RNA in the tears of polymerase chain reaction (PCR)-confirmed SARS-CoV-2 positive patients. METHODS: We performed a prospective case series study of hospitalized patients who have been confirmed SARS-CoV-2 positive by oropharyngeal swab within the previous 5 days. Tear samples obtained with a laboratory capillary and oropharyngeal swabs were analyzed by real-time PCR using the Altona SARS-CoV-2 Assay or the Roche SARS-CoV-2 LightMix PCR, depending on the availability. Patient history was documented, and ophthalmoscopy was used to assess for ocular surface disease. RESULTS: Of all 18 patients recruited in April 2020, 5 suffered from respiratory failure and were submitted to an intensive care unit. None of our patients had signs of viral conjunctivitis although all patients in intensive care showed chemosis and conjunctival hyperemia because of third-spacing or fluid overload. The presence of coronavirus RNA was confirmed by PCR in 5 of 18 patients (28%) in tears and 72% for oropharyngeal swabs. CONCLUSIONS: Using a tear fluid sampling technique similar to oropharyngeal lavage presents a higher percentage of SARS-CoV-2 positive tears in contrast to earlier reports that used a conjunctival swab. This does not automatically indicate viral shedding in ocular tissue or contagiousness of tear fluid. SN - 1536-4798 UR - https://www.unboundmedicine.com/medline/citation/32897895/Detection_of_Coronavirus_in_Tear_Samples_of_Hospitalized_Patients_With_Confirmed_SARS_CoV_2_From_Oropharyngeal_Swabs_ DB - PRIME DP - Unbound Medicine ER -