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Expansion of plasmablasts and loss of memory B cells in peripheral blood from COVID-19 patients with pneumonia.
Eur J Immunol. 2020 09; 50(9):1283-1294.EJ

Abstract

Studies on the interactions between SARS-CoV-2 and humoral immunity are fundamental to elaborate effective therapies including vaccines. We used polychromatic flow cytometry, coupled with unsupervised data analysis and principal component analysis (PCA), to interrogate B cells in untreated patients with COVID-19 pneumonia. COVID-19 patients displayed normal plasma levels of the main immunoglobulin classes, of antibodies against common antigens or against antigens present in common vaccines. However, we found a decreased number of total and naïve B cells, along with decreased percentages and numbers of memory switched and unswitched B cells. On the contrary, IgM+ and IgM- plasmablasts were significantly increased. In vitro cell activation revealed that B lymphocytes showed a normal proliferation index and number of dividing cells per cycle. PCA indicated that B-cell number, naive and memory B cells but not plasmablasts clustered with patients who were discharged, while plasma IgM level, C-reactive protein, D-dimer, and SOFA score with those who died. In patients with pneumonia, the derangement of the B-cell compartment could be one of the causes of the immunological failure to control SARS-Cov2, have a relevant influence on several pathways, organs and systems, and must be considered to develop vaccine strategies.

Authors+Show Affiliations

Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Department of Anesthesia and Intensive Care, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Clinical Microbiology Unit, AOU Policlinico, Modena, Italy.Laboratory of Immunology, Department of Medicine, University of Udine, Udine, Italy.Department of Anesthesia and Intensive Care, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy. National Institute for Cardiovascular Research, Bologna, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32910469

Citation

De Biasi, Sara, et al. "Expansion of Plasmablasts and Loss of Memory B Cells in Peripheral Blood From COVID-19 Patients With Pneumonia." European Journal of Immunology, vol. 50, no. 9, 2020, pp. 1283-1294.
De Biasi S, Lo Tartaro D, Meschiari M, et al. Expansion of plasmablasts and loss of memory B cells in peripheral blood from COVID-19 patients with pneumonia. Eur J Immunol. 2020;50(9):1283-1294.
De Biasi, S., Lo Tartaro, D., Meschiari, M., Gibellini, L., Bellinazzi, C., Borella, R., Fidanza, L., Mattioli, M., Paolini, A., Gozzi, L., Jaacoub, D., Faltoni, M., Volpi, S., Milić, J., Sita, M., Sarti, M., Pucillo, C., Girardis, M., Guaraldi, G., ... Cossarizza, A. (2020). Expansion of plasmablasts and loss of memory B cells in peripheral blood from COVID-19 patients with pneumonia. European Journal of Immunology, 50(9), 1283-1294. https://doi.org/10.1002/eji.202048838
De Biasi S, et al. Expansion of Plasmablasts and Loss of Memory B Cells in Peripheral Blood From COVID-19 Patients With Pneumonia. Eur J Immunol. 2020;50(9):1283-1294. PubMed PMID: 32910469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of plasmablasts and loss of memory B cells in peripheral blood from COVID-19 patients with pneumonia. AU - De Biasi,Sara, AU - Lo Tartaro,Domenico, AU - Meschiari,Marianna, AU - Gibellini,Lara, AU - Bellinazzi,Caterina, AU - Borella,Rebecca, AU - Fidanza,Lucia, AU - Mattioli,Marco, AU - Paolini,Annamaria, AU - Gozzi,Licia, AU - Jaacoub,Dina, AU - Faltoni,Matteo, AU - Volpi,Sara, AU - Milić,Jovana, AU - Sita,Marco, AU - Sarti,Mario, AU - Pucillo,Carlo, AU - Girardis,Massimo, AU - Guaraldi,Giovanni, AU - Mussini,Cristina, AU - Cossarizza,Andrea, Y1 - 2020/08/13/ PY - 2020/06/23/received PY - 2020/07/09/revised PY - 2020/07/23/accepted PY - 2020/9/10/entrez PY - 2020/9/11/pubmed PY - 2020/9/23/medline KW - B cells KW - COVID‐19 KW - Coronavirus KW - SARS‐CoV‐2 KW - Uniform Manifold Approximation and Projection (UMAP) KW - carboxyfluorescein succinimidyl ester CFSE KW - plasmablasts KW - principal component analysis (PCA) KW - principal components (PCs) SP - 1283 EP - 1294 JF - European journal of immunology JO - Eur J Immunol VL - 50 IS - 9 N2 - Studies on the interactions between SARS-CoV-2 and humoral immunity are fundamental to elaborate effective therapies including vaccines. We used polychromatic flow cytometry, coupled with unsupervised data analysis and principal component analysis (PCA), to interrogate B cells in untreated patients with COVID-19 pneumonia. COVID-19 patients displayed normal plasma levels of the main immunoglobulin classes, of antibodies against common antigens or against antigens present in common vaccines. However, we found a decreased number of total and naïve B cells, along with decreased percentages and numbers of memory switched and unswitched B cells. On the contrary, IgM+ and IgM- plasmablasts were significantly increased. In vitro cell activation revealed that B lymphocytes showed a normal proliferation index and number of dividing cells per cycle. PCA indicated that B-cell number, naive and memory B cells but not plasmablasts clustered with patients who were discharged, while plasma IgM level, C-reactive protein, D-dimer, and SOFA score with those who died. In patients with pneumonia, the derangement of the B-cell compartment could be one of the causes of the immunological failure to control SARS-Cov2, have a relevant influence on several pathways, organs and systems, and must be considered to develop vaccine strategies. SN - 1521-4141 UR - https://www.unboundmedicine.com/medline/citation/32910469/Expansion_of_plasmablasts_and_loss_of_memory_B_cells_in_peripheral_blood_from_COVID_19_patients_with_pneumonia_ L2 - https://doi.org/10.1002/eji.202048838 DB - PRIME DP - Unbound Medicine ER -