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Absence of effect of heparin on insulin secretion.
Artif Organs. 1988 Apr; 12(2):137-42.AO

Abstract

Bioartificial pancreatic devices containing isolated islets of Langerhans have been designed, in which the blood of the recipient circulates in contact with an artificial membrane, protecting the islets against immune rejection. This system assumes that heparin, required to prevent blood clotting, does not alter insulin secretion. However, heparin has been reported to inhibit in vitro insulin secretion by rat islets and to suppress in vivo insulin secretion in dogs. Therefore, the following evaluation was made on the effect of different heparin preparations on insulin secretion. (a) Isolated rat islets of Langerhans were perfused or incubated in the absence or presence of 20 micrograms/ml heparin; insulin secretion in response to a stimulation by glucose 20 mM was not altered by the presence of heparin. (b) Insulin secretion by an insulin-secreting cell line (RINm5F) in response to leucine and theophylline was not suppressed by heparin up to 100 micrograms/ml concentration. However, an inhibitory effect was observed at 200 micrograms/ml, which is 100 times higher than the heparin concentration commonly used for therapeutic use. (c) Neither in normal rats nor in dogs did heparin alter portal plasma insulin levels and the increase in plasma insulin following an intravenous injection of glucose. In conclusion, these data do not confirm the formerly observed inhibitory effect of heparin, which can therefore be used for the in vivo evaluation of a bioartificial pancreas.

Authors+Show Affiliations

Service de Diabétologie, Hotel-Dieu, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3291828

Citation

Moussy, F, et al. "Absence of Effect of Heparin On Insulin Secretion." Artificial Organs, vol. 12, no. 2, 1988, pp. 137-42.
Moussy F, Velho G, Aubert N, et al. Absence of effect of heparin on insulin secretion. Artif Organs. 1988;12(2):137-42.
Moussy, F., Velho, G., Aubert, N., Chenard, P. S., Lepeintre, J., Serne, H., Jozefowicz, M., Reach, G., Freyse, E. J., & Friedrich, M. (1988). Absence of effect of heparin on insulin secretion. Artificial Organs, 12(2), 137-42.
Moussy F, et al. Absence of Effect of Heparin On Insulin Secretion. Artif Organs. 1988;12(2):137-42. PubMed PMID: 3291828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of effect of heparin on insulin secretion. A1 - Moussy,F, AU - Velho,G, AU - Aubert,N, AU - Chenard,P S, AU - Lepeintre,J, AU - Serne,H, AU - Jozefowicz,M, AU - Reach,G, AU - Freyse,E J, AU - Friedrich,M, PY - 1988/4/1/pubmed PY - 1988/4/1/medline PY - 1988/4/1/entrez SP - 137 EP - 42 JF - Artificial organs JO - Artif Organs VL - 12 IS - 2 N2 - Bioartificial pancreatic devices containing isolated islets of Langerhans have been designed, in which the blood of the recipient circulates in contact with an artificial membrane, protecting the islets against immune rejection. This system assumes that heparin, required to prevent blood clotting, does not alter insulin secretion. However, heparin has been reported to inhibit in vitro insulin secretion by rat islets and to suppress in vivo insulin secretion in dogs. Therefore, the following evaluation was made on the effect of different heparin preparations on insulin secretion. (a) Isolated rat islets of Langerhans were perfused or incubated in the absence or presence of 20 micrograms/ml heparin; insulin secretion in response to a stimulation by glucose 20 mM was not altered by the presence of heparin. (b) Insulin secretion by an insulin-secreting cell line (RINm5F) in response to leucine and theophylline was not suppressed by heparin up to 100 micrograms/ml concentration. However, an inhibitory effect was observed at 200 micrograms/ml, which is 100 times higher than the heparin concentration commonly used for therapeutic use. (c) Neither in normal rats nor in dogs did heparin alter portal plasma insulin levels and the increase in plasma insulin following an intravenous injection of glucose. In conclusion, these data do not confirm the formerly observed inhibitory effect of heparin, which can therefore be used for the in vivo evaluation of a bioartificial pancreas. SN - 0160-564X UR - https://www.unboundmedicine.com/medline/citation/3291828/Absence_of_effect_of_heparin_on_insulin_secretion_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0160-564X&date=1988&volume=12&issue=2&spage=137 DB - PRIME DP - Unbound Medicine ER -