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Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities.
Clin Microbiol Infect. 2020 Dec; 26(12):1658-1662.CM

Abstract

OBJECTIVES

Environmental surfaces have been suggested as likely contributors in the transmission of COVID-19. This study assessed the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminating surfaces and objects in two hospital isolation units and a quarantine hotel.

METHODS

SARS-CoV-2 virus stability and infectivity on non-porous surfaces was tested under controlled laboratory conditions. Surface and air sampling were conducted at two COVID-19 isolation units and in a quarantine hotel. Viral RNA was detected by RT-PCR and infectivity was assessed by VERO E6 CPE test.

RESULTS

In laboratory-controlled conditions, SARS-CoV-2 gradually lost its infectivity completely by day 4 at ambient temperature, and the decay rate of viral viability on surfaces directly correlated with increase in temperature. Viral RNA was detected in 29/55 surface samples (52.7%) and 16/42 surface samples (38%) from the surroundings of symptomatic COVID-19 patients in isolation units of two hospitals and in a quarantine hotel for asymptomatic and very mild COVID-19 patients. None of the surface and air samples from the three sites (0/97) were found to contain infectious titres of SARS-Cov-2 on tissue culture assay.

CONCLUSIONS

Despite prolonged viability of SARS-CoV-2 under laboratory-controlled conditions, uncultivable viral contamination of inanimate surfaces might suggest low feasibility for indirect fomite transmission.

Authors+Show Affiliations

Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Infectious Diseases Unit, Assuta Ashdod University Hospital, Ashdod, Israel; Faculty of Health Sciences, Ben-Gurion University in the Negev, Beer-Sheba, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Infectious Diseases Unit, Laniado Hospital, Netanya, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel. Electronic address: shayw@iibr.gov.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32919072

Citation

Ben-Shmuel, Amir, et al. "Detection and Infectivity Potential of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Environmental Contamination in Isolation Units and Quarantine Facilities." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 26, no. 12, 2020, pp. 1658-1662.
Ben-Shmuel A, Brosh-Nissimov T, Glinert I, et al. Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities. Clin Microbiol Infect. 2020;26(12):1658-1662.
Ben-Shmuel, A., Brosh-Nissimov, T., Glinert, I., Bar-David, E., Sittner, A., Poni, R., Cohen, R., Achdout, H., Tamir, H., Yahalom-Ronen, Y., Politi, B., Melamed, S., Vitner, E., Cherry, L., Israeli, O., Beth-Din, A., Paran, N., Israely, T., Yitzhaki, S., ... Weiss, S. (2020). Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 26(12), 1658-1662. https://doi.org/10.1016/j.cmi.2020.09.004
Ben-Shmuel A, et al. Detection and Infectivity Potential of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Environmental Contamination in Isolation Units and Quarantine Facilities. Clin Microbiol Infect. 2020;26(12):1658-1662. PubMed PMID: 32919072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities. AU - Ben-Shmuel,Amir, AU - Brosh-Nissimov,Tal, AU - Glinert,Itai, AU - Bar-David,Elad, AU - Sittner,Assa, AU - Poni,Reut, AU - Cohen,Regev, AU - Achdout,Hagit, AU - Tamir,Hadas, AU - Yahalom-Ronen,Yfat, AU - Politi,Boaz, AU - Melamed,Sharon, AU - Vitner,Einat, AU - Cherry,Lilach, AU - Israeli,Ofir, AU - Beth-Din,Adi, AU - Paran,Nir, AU - Israely,Tomer, AU - Yitzhaki,Shmuel, AU - Levy,Haim, AU - Weiss,Shay, Y1 - 2020/09/10/ PY - 2020/06/08/received PY - 2020/08/26/revised PY - 2020/09/01/accepted PY - 2020/9/13/pubmed PY - 2020/12/15/medline PY - 2020/9/12/entrez KW - COVID-19 KW - Contamination KW - Coronavirus KW - SARS-CoV-2 KW - Surface KW - Viability SP - 1658 EP - 1662 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 26 IS - 12 N2 - OBJECTIVES: Environmental surfaces have been suggested as likely contributors in the transmission of COVID-19. This study assessed the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminating surfaces and objects in two hospital isolation units and a quarantine hotel. METHODS: SARS-CoV-2 virus stability and infectivity on non-porous surfaces was tested under controlled laboratory conditions. Surface and air sampling were conducted at two COVID-19 isolation units and in a quarantine hotel. Viral RNA was detected by RT-PCR and infectivity was assessed by VERO E6 CPE test. RESULTS: In laboratory-controlled conditions, SARS-CoV-2 gradually lost its infectivity completely by day 4 at ambient temperature, and the decay rate of viral viability on surfaces directly correlated with increase in temperature. Viral RNA was detected in 29/55 surface samples (52.7%) and 16/42 surface samples (38%) from the surroundings of symptomatic COVID-19 patients in isolation units of two hospitals and in a quarantine hotel for asymptomatic and very mild COVID-19 patients. None of the surface and air samples from the three sites (0/97) were found to contain infectious titres of SARS-Cov-2 on tissue culture assay. CONCLUSIONS: Despite prolonged viability of SARS-CoV-2 under laboratory-controlled conditions, uncultivable viral contamination of inanimate surfaces might suggest low feasibility for indirect fomite transmission. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/32919072/Detection_and_infectivity_potential_of_severe_acute_respiratory_syndrome_coronavirus_2__SARS_CoV_2__environmental_contamination_in_isolation_units_and_quarantine_facilities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(20)30532-2 DB - PRIME DP - Unbound Medicine ER -