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Immunological cross-recognition and neutralization studies of Micrurus mipartitus and Micrurus dumerilii venoms by two therapeutic equine antivenoms.
Biologicals. 2020 Nov; 68:40-45.B

Abstract

New world Coral snakes comprise 82 species of medical importance distributed from southeastern United States to Argentina. In Colombia, Micrurus mipartitus and M. dumerilii are responsible for most coral snakebite accidents. Although infrequent, the severity of these envenomings, as well as the limited information available on the neutralizing coverage of commercially available antivenoms, underscores the need to perform studies to assess the cross-neutralizing ability of these life-saving immunobiologicals. In the present work, we evaluated the cross-recognition and neutralization ability of two equine therapeutic antivenoms: PROBIOL and SAC-ICP. PROBIOL antivenom showed cross-recognition towards both M. mipartitus and M. dumerilii venoms, with a significantly higher binding to the latter in both whole-venom ELISA and fractionated-venom immunoprofiling. In contrast, SAC-ICP antivenom cross-recognized M. dumerilii venom, but not that of M. mipartitus. Lethality of M. dumerilii venom was neutralized by both antivenoms, with a slightly higher potency for the SAC-ICP antivenom. However, the lethality of M. mipartitus venom was not neutralized by any of the two antivenoms. Results uncover the need to include M. mipartitus venom, or its most relevant toxins, in the production of coral snake antivenoms to be used in Colombia, to assure the neutralizing coverage for this species.

Authors+Show Affiliations

Programa de Ofidismo y Escorpionismo, Universidad de Antioquia, Medellín, Colombia. Electronic address: jessica.rey@udea.edu.co.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32928631

Citation

Rey-Suárez, Paola, and Bruno Lomonte. "Immunological Cross-recognition and Neutralization Studies of Micrurus Mipartitus and Micrurus Dumerilii Venoms By Two Therapeutic Equine Antivenoms." Biologicals : Journal of the International Association of Biological Standardization, vol. 68, 2020, pp. 40-45.
Rey-Suárez P, Lomonte B. Immunological cross-recognition and neutralization studies of Micrurus mipartitus and Micrurus dumerilii venoms by two therapeutic equine antivenoms. Biologicals. 2020;68:40-45.
Rey-Suárez, P., & Lomonte, B. (2020). Immunological cross-recognition and neutralization studies of Micrurus mipartitus and Micrurus dumerilii venoms by two therapeutic equine antivenoms. Biologicals : Journal of the International Association of Biological Standardization, 68, 40-45. https://doi.org/10.1016/j.biologicals.2020.08.011
Rey-Suárez P, Lomonte B. Immunological Cross-recognition and Neutralization Studies of Micrurus Mipartitus and Micrurus Dumerilii Venoms By Two Therapeutic Equine Antivenoms. Biologicals. 2020;68:40-45. PubMed PMID: 32928631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunological cross-recognition and neutralization studies of Micrurus mipartitus and Micrurus dumerilii venoms by two therapeutic equine antivenoms. AU - Rey-Suárez,Paola, AU - Lomonte,Bruno, Y1 - 2020/09/11/ PY - 2020/05/25/received PY - 2020/08/13/revised PY - 2020/08/29/accepted PY - 2020/9/16/pubmed PY - 2021/8/31/medline PY - 2020/9/15/entrez KW - Antivenom KW - Coral snake KW - Cross-neutralization KW - Micrurus dumerilii KW - Micrurus mipartitus SP - 40 EP - 45 JF - Biologicals : journal of the International Association of Biological Standardization JO - Biologicals VL - 68 N2 - New world Coral snakes comprise 82 species of medical importance distributed from southeastern United States to Argentina. In Colombia, Micrurus mipartitus and M. dumerilii are responsible for most coral snakebite accidents. Although infrequent, the severity of these envenomings, as well as the limited information available on the neutralizing coverage of commercially available antivenoms, underscores the need to perform studies to assess the cross-neutralizing ability of these life-saving immunobiologicals. In the present work, we evaluated the cross-recognition and neutralization ability of two equine therapeutic antivenoms: PROBIOL and SAC-ICP. PROBIOL antivenom showed cross-recognition towards both M. mipartitus and M. dumerilii venoms, with a significantly higher binding to the latter in both whole-venom ELISA and fractionated-venom immunoprofiling. In contrast, SAC-ICP antivenom cross-recognized M. dumerilii venom, but not that of M. mipartitus. Lethality of M. dumerilii venom was neutralized by both antivenoms, with a slightly higher potency for the SAC-ICP antivenom. However, the lethality of M. mipartitus venom was not neutralized by any of the two antivenoms. Results uncover the need to include M. mipartitus venom, or its most relevant toxins, in the production of coral snake antivenoms to be used in Colombia, to assure the neutralizing coverage for this species. SN - 1095-8320 UR - https://www.unboundmedicine.com/medline/citation/32928631/Immunological_cross_recognition_and_neutralization_studies_of_Micrurus_mipartitus_and_Micrurus_dumerilii_venoms_by_two_therapeutic_equine_antivenoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1045-1056(20)30109-3 DB - PRIME DP - Unbound Medicine ER -