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Antibody binding to SARS-CoV-2 S glycoprotein correlates with, but does not predict neutralization.
bioRxiv. 2020 Sep 08B

Abstract

Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals as well as S-specific monoclonal antibodies were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to binding of S glycoprotein in the context of viral particles remains to be established. Here we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA and neutralization assays we observed a strong correlation between these two parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of viral particles is required but not sufficient to mediate neutralization. Altogether, our results highlights the importance of better understanding the inactivation of S by plasma and neutralizing antibodies.

Authors

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Pub Type(s)

Preprint

Language

eng

PubMed ID

32935094

Citation

Ding, Shilei, et al. "Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates With, but Does Not Predict Neutralization." BioRxiv : the Preprint Server for Biology, 2020.
Ding S, Laumaea A, Gasser R, et al. Antibody binding to SARS-CoV-2 S glycoprotein correlates with, but does not predict neutralization. bioRxiv. 2020.
Ding, S., Laumaea, A., Gasser, R., Medjahed, H., Pancera, M., Stamatatos, L., McGuire, A., Bazin, R., & Finzi, A. (2020). Antibody binding to SARS-CoV-2 S glycoprotein correlates with, but does not predict neutralization. BioRxiv : the Preprint Server for Biology. https://doi.org/10.1101/2020.09.08.287482
Ding S, et al. Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates With, but Does Not Predict Neutralization. bioRxiv. 2020 Sep 8; PubMed PMID: 32935094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody binding to SARS-CoV-2 S glycoprotein correlates with, but does not predict neutralization. AU - Ding,Shilei, AU - Laumaea,Annemarie, AU - Gasser,Romain, AU - Medjahed,Halima, AU - Pancera,Marie, AU - Stamatatos,Leonidas, AU - McGuire,Andrew, AU - Bazin,Renée, AU - Finzi,Andrés, Y1 - 2020/09/08/ PY - 2020/9/16/entrez PY - 2020/9/17/pubmed PY - 2020/9/17/medline JF - bioRxiv : the preprint server for biology JO - bioRxiv N2 - Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals as well as S-specific monoclonal antibodies were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to binding of S glycoprotein in the context of viral particles remains to be established. Here we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA and neutralization assays we observed a strong correlation between these two parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of viral particles is required but not sufficient to mediate neutralization. Altogether, our results highlights the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. UR - https://www.unboundmedicine.com/medline/citation/32935094/Antibody_binding_to_SARS_CoV_2_S_glycoprotein_correlates_with_but_does_not_predict_neutralization_ L2 - https://doi.org/10.1101/2020.09.08.287482 DB - PRIME DP - Unbound Medicine ER -
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