Tags

Type your tag names separated by a space and hit enter

Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system.
Nanoscale. 2020 Oct 01; 12(37):19149-19158.N

Abstract

The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5-100 μg mL-1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 μg mL-1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.

Authors+Show Affiliations

Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Breast Cancer Centre, Shanghai East Hospital, Tongji University, Shanghai, 200092, P. R. China. shishuo@tongji.edu.cn.Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Breast Cancer Centre, Shanghai East Hospital, Tongji University, Shanghai, 200092, P. R. China. shishuo@tongji.edu.cn.Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Breast Cancer Centre, Shanghai East Hospital, Tongji University, Shanghai, 200092, P. R. China. shishuo@tongji.edu.cn.Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Breast Cancer Centre, Shanghai East Hospital, Tongji University, Shanghai, 200092, P. R. China. shishuo@tongji.edu.cn.Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Breast Cancer Centre, Shanghai East Hospital, Tongji University, Shanghai, 200092, P. R. China. shishuo@tongji.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32936174

Citation

Afthab, Jouharsha, et al. "Hepatoprotective Angelica Sinensis Silver Nanoformulation Against Multidrug Resistant Bacteria and the Integration of a Multicomponent Logic Gate System." Nanoscale, vol. 12, no. 37, 2020, pp. 19149-19158.
Afthab J, Khatoon N, Zhou L, et al. Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system. Nanoscale. 2020;12(37):19149-19158.
Afthab, J., Khatoon, N., Zhou, L., Yao, T., & Shi, S. (2020). Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system. Nanoscale, 12(37), 19149-19158. https://doi.org/10.1039/d0nr04744a
Afthab J, et al. Hepatoprotective Angelica Sinensis Silver Nanoformulation Against Multidrug Resistant Bacteria and the Integration of a Multicomponent Logic Gate System. Nanoscale. 2020 Oct 1;12(37):19149-19158. PubMed PMID: 32936174.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system. AU - Afthab,Jouharsha, AU - Khatoon,Nafeesa, AU - Zhou,Lulu, AU - Yao,Tianming, AU - Shi,Shuo, PY - 2020/9/17/pubmed PY - 2021/5/15/medline PY - 2020/9/16/entrez SP - 19149 EP - 19158 JF - Nanoscale JO - Nanoscale VL - 12 IS - 37 N2 - The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5-100 μg mL-1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 μg mL-1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria. SN - 2040-3372 UR - https://www.unboundmedicine.com/medline/citation/32936174/Hepatoprotective_Angelica_sinensis_silver_nanoformulation_against_multidrug_resistant_bacteria_and_the_integration_of_a_multicomponent_logic_gate_system. L2 - https://doi.org/10.1039/d0nr04744a DB - PRIME DP - Unbound Medicine ER -