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Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms.
Infection. 2020 Sep 17 [Online ahead of print]I

Abstract

PURPOSE

To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.

METHODS

Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.

RESULTS

A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2-27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.

CONCLUSION

Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.

Authors+Show Affiliations

Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China. Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.Department of Ophthalmology, United Christian Hospital, Hong Kong Special Administrative Region, China.Department of Medicine, United Christian Hospital, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Hong Kong Special Administrative Region, China.Department of Pathology, United Christian Hospital, Hong Kong Special Administrative Region, China.Department of Medicine, Ruttonjee and Tang Shiu Kin Hospital, Hong Kong Special Administrative Region, China.Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong Special Administrative Region, China.Department of Medicine, Queen Mary Hospital, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China. ivanhung@hku.hk. Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. ivanhung@hku.hk. Department of Medicine, Queen Mary Hospital, Hong Kong Special Administrative Region, China. ivanhung@hku.hk.State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China. Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32944840

Citation

Li, Xin, et al. "Detection of SARS-CoV-2 in Conjunctival Secretions From Patients Without Ocular Symptoms." Infection, 2020.
Li X, Chan JF, Li KK, et al. Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms. Infection. 2020.
Li, X., Chan, J. F., Li, K. K., Tso, E. Y., Yip, C. C., Sridhar, S., Chung, T. W., Chiu, K. H., Hung, D. L., Wu, A. K., Chau, S. K., Liu, R., Lung, K. C., Tam, A. R., Cheng, V. C., To, K. K., Chan, K. H., Hung, I. F., & Yuen, K. Y. (2020). Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms. Infection. https://doi.org/10.1007/s15010-020-01524-2
Li X, et al. Detection of SARS-CoV-2 in Conjunctival Secretions From Patients Without Ocular Symptoms. Infection. 2020 Sep 17; PubMed PMID: 32944840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms. AU - Li,Xin, AU - Chan,Jasper Fuk-Woo, AU - Li,Kenneth Kai-Wang, AU - Tso,Eugene Yuk-Keung, AU - Yip,Cyril Chik-Yan, AU - Sridhar,Siddharth, AU - Chung,Tom Wai-Hin, AU - Chiu,Kelvin Hei-Yeung, AU - Hung,Derek Ling-Lung, AU - Wu,Alan Ka-Lun, AU - Chau,Sandy Ka-Yee, AU - Liu,Raymond, AU - Lung,Kwok-Cheung, AU - Tam,Anthony Raymond, AU - Cheng,Vincent Chi-Chung, AU - To,Kelvin Kai-Wang, AU - Chan,Kwok-Hung, AU - Hung,Ivan Fan-Ngai, AU - Yuen,Kwok-Yung, Y1 - 2020/09/17/ PY - 2020/07/07/received PY - 2020/09/07/accepted PY - 2020/9/18/entrez PY - 2020/9/19/pubmed PY - 2020/9/19/medline KW - Asymptomatic KW - Conjunctiva KW - RT-PCR KW - SARS-CoV-2 KW - Shedding JF - Infection JO - Infection N2 - PURPOSE: To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms. METHODS: Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture. RESULTS: A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2-27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs. CONCLUSION: Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2. SN - 1439-0973 UR - https://www.unboundmedicine.com/medline/citation/32944840/Detection_of_SARS_CoV_2_in_conjunctival_secretions_from_patients_without_ocular_symptoms_ L2 - https://doi.org/10.1007/s15010-020-01524-2 DB - PRIME DP - Unbound Medicine ER -
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