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Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics.
Anal Chem. 2020 10 20; 92(20):13647-13651.AC

Abstract

The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).

Authors+Show Affiliations

Department of Chemistry, University of Oxford, Oxford OX1 3QZ, United Kingdom.Nuffield Department of Clinical Neurosciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.Nuffield Department of Clinical Neurosciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.Department of Chemistry, University of Oxford, Oxford OX1 3QZ, United Kingdom.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32945162

Citation

Fu, Ying, et al. "Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics." Analytical Chemistry, vol. 92, no. 20, 2020, pp. 13647-13651.
Fu Y, Jiang C, Tofaris GK, et al. Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics. Anal Chem. 2020;92(20):13647-13651.
Fu, Y., Jiang, C., Tofaris, G. K., & Davis, J. J. (2020). Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics. Analytical Chemistry, 92(20), 13647-13651. https://doi.org/10.1021/acs.analchem.0c03092
Fu Y, et al. Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics. Anal Chem. 2020 10 20;92(20):13647-13651. PubMed PMID: 32945162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics. AU - Fu,Ying, AU - Jiang,Cheng, AU - Tofaris,George K, AU - Davis,Jason J, Y1 - 2020/10/05/ PY - 2020/9/19/pubmed PY - 2021/2/18/medline PY - 2020/9/18/entrez SP - 13647 EP - 13651 JF - Analytical chemistry JO - Anal Chem VL - 92 IS - 20 N2 - The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC). SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/32945162/Facile_Impedimetric_Analysis_of_Neuronal_Exosome_Markers_in_Parkinson's_Disease_Diagnostics_ L2 - https://doi.org/10.1021/acs.analchem.0c03092 DB - PRIME DP - Unbound Medicine ER -