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Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay.
J Virol Methods. 2020 12; 286:113974.JV

Abstract

OBJECTIVE

Low viral load from patients infected with SARS-CoV-2 during infection late stage easily lead to false negative nucleic acid testing results, thus having great challenges to the prevention and control of the current pandemic. In present study, we mainly aimed to evaluate specimen types and specimen collection timepoint on the positive detection of 2019 novel coronavirus from patients at infection late stage based on RT-PCR testing.

METHODS

Paired nasopharyngeal swabs, nasal swabs, oropharyngeal swabs and anal swabs were collected from patients infected with SARS-CoV-2 during infection late stage before washing in the morning and afternoon on the same day. Then virus RNA was extracted and tested for 2019-nCoV identification by RT-PCR within 24 h.

RESULTS

Viral load was low at late infection stage. Specimens collected before washing in the morning would increase the detection ratio of 2019-nCoV. Detection ratio of nasopharyngeal swab [65 (95 % CI: 49.51-77.87) vs 42.5(95 % CI: 28.51-57.8)] or nasal swab [57.5 (95 % CI: 42.2-71.49) vs 35 (95 % CI: 22.13-50.49)] is higher not only than oropharyngeal swab[22.5 (95 % CI: 12.32-37.5) vs 7.5 (95 % CI: 2.58-19.86)], but also anal swab[2.5 (95 % CI: 0.44-12.88) vs 5 (95 % CI: 1.38-16.5)].

CONCLUSIONS

In summary, our research discovers that nasopharyngeal or nasal swab collected before washing in the morning might be more suitable for detecting of large-scale specimens from patients infected with low SARS-CoV-2 load during infection late stage. Those results could facilitate other laboratories in collecting appropriate specimens for improving detection of SARS-CoV-2 from patients during infection late stage as well as initially screening.

Authors+Show Affiliations

The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China. Electronic address: lj1988cby@126.com.The First College of Clinical Medical Science, China Three Gorges University, Yichang, China; Yichang Central People's Hospital, China. Electronic address: lchlgj2004@aliyun.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32949663

Citation

Liu, Min, et al. "Value of Swab Types and Collection Time On SARS-COV-2 Detection Using RT-PCR Assay." Journal of Virological Methods, vol. 286, 2020, p. 113974.
Liu M, Li Q, Zhou J, et al. Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay. J Virol Methods. 2020;286:113974.
Liu, M., Li, Q., Zhou, J., Ai, W., Zheng, X., Zeng, J., Liu, Y., Xiang, X., Guo, R., Li, X., Wu, X., Xu, H., Jiang, L., Zhang, H., Chen, J., Tian, L., Luo, J., & Luo, C. (2020). Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay. Journal of Virological Methods, 286, 113974. https://doi.org/10.1016/j.jviromet.2020.113974
Liu M, et al. Value of Swab Types and Collection Time On SARS-COV-2 Detection Using RT-PCR Assay. J Virol Methods. 2020;286:113974. PubMed PMID: 32949663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay. AU - Liu,Min, AU - Li,Qianyuan, AU - Zhou,Jun, AU - Ai,Wen, AU - Zheng,Xiaoling, AU - Zeng,Jingjing, AU - Liu,Yuwen, AU - Xiang,Xiying, AU - Guo,Rong, AU - Li,Xiaoyin, AU - Wu,Xiandi, AU - Xu,Haiying, AU - Jiang,Ling, AU - Zhang,Huaqin, AU - Chen,Jing, AU - Tian,Lili, AU - Luo,Jun, AU - Luo,Chunhua, Y1 - 2020/09/16/ PY - 2020/05/05/received PY - 2020/07/27/revised PY - 2020/09/14/accepted PY - 2020/9/20/pubmed PY - 2020/12/1/medline PY - 2020/9/19/entrez KW - Collection timepoint KW - Positive detection rate KW - RT-PCR KW - SARS-Cov-2 KW - Specimen types SP - 113974 EP - 113974 JF - Journal of virological methods JO - J Virol Methods VL - 286 N2 - OBJECTIVE: Low viral load from patients infected with SARS-CoV-2 during infection late stage easily lead to false negative nucleic acid testing results, thus having great challenges to the prevention and control of the current pandemic. In present study, we mainly aimed to evaluate specimen types and specimen collection timepoint on the positive detection of 2019 novel coronavirus from patients at infection late stage based on RT-PCR testing. METHODS: Paired nasopharyngeal swabs, nasal swabs, oropharyngeal swabs and anal swabs were collected from patients infected with SARS-CoV-2 during infection late stage before washing in the morning and afternoon on the same day. Then virus RNA was extracted and tested for 2019-nCoV identification by RT-PCR within 24 h. RESULTS: Viral load was low at late infection stage. Specimens collected before washing in the morning would increase the detection ratio of 2019-nCoV. Detection ratio of nasopharyngeal swab [65 (95 % CI: 49.51-77.87) vs 42.5(95 % CI: 28.51-57.8)] or nasal swab [57.5 (95 % CI: 42.2-71.49) vs 35 (95 % CI: 22.13-50.49)] is higher not only than oropharyngeal swab[22.5 (95 % CI: 12.32-37.5) vs 7.5 (95 % CI: 2.58-19.86)], but also anal swab[2.5 (95 % CI: 0.44-12.88) vs 5 (95 % CI: 1.38-16.5)]. CONCLUSIONS: In summary, our research discovers that nasopharyngeal or nasal swab collected before washing in the morning might be more suitable for detecting of large-scale specimens from patients infected with low SARS-CoV-2 load during infection late stage. Those results could facilitate other laboratories in collecting appropriate specimens for improving detection of SARS-CoV-2 from patients during infection late stage as well as initially screening. SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/32949663/Value_of_swab_types_and_collection_time_on_SARS_COV_2_detection_using_RT_PCR_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(20)30226-3 DB - PRIME DP - Unbound Medicine ER -