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Functional and morphological alterations in a glaucoma model of acute ocular hypertension.
Prog Brain Res. 2020; 256(1):1-29.PB

Abstract

To study short and long-term effects of acute ocular hypertension (AOHT) on inner and outer retinal layers, in adult Sprague-Dawley rats AOHT (87mmHg) was induced for 90min and the retinas were examined longitudinally in vivo with electroretinogram (ERG) recordings and optical coherent tomography (OCT) from 1 to 90 days (d). Ex vivo, the retinas were analyzed for rod (RBC) and cone (CBC) bipolar cells, with antibodies against protein kinase Cα and recoverin, respectively in cross sections, and for cones, horizontal (HZ) and ganglion (RGC) cells with antibodies against arrestin, calbindin and Brn3a, respectively in wholemounts. The inner retina thinned progressively up to 7d with no further changes, while the external retina had a normal thickness until 30d, with a 20% thinning between 30 and 90d. Functionally, the a-wave showed an initial reduction by 24h and a further reduction from 30 to 90d. All other main ERG waves were significantly reduced by 1d without significant recovery by 90d. Radial sections showed a normal population of RBCs but their terminals were reduced. The CBCs showed a progressive decrease with a loss of 56% by 30d. In wholemount retinas, RGCs diminished to 40% by 3d and to 16% by 30d without further loss. Cones diminished to 58% and 35% by 3 and 7d, respectively and further decreased between 30 and 90d. HZs showed normal values throughout the study. In conclusion, AOHT affects both the inner and outer retina, with a more pronounced degeneration of the cone than the rod pathway.

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Authors+Show Affiliations

Gallego-Ortega A
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Norte-Muñoz M
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Miralles de Imperial-Ollero JA
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Bernal-Garro JM
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Valiente-Soriano FJ
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
de la Villa Polo P
Department of Systems Biology, University of Alcalá, Madrid, Spain; Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain.
Avilés-Trigueros M
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Villegas-Pérez MP
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain.
Vidal-Sanz M
Department of Ophthalmology, University of Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain. Electronic address: manuel.vidal@um.es.

MeSH

Acute DiseaseAnimalsDisease Models, AnimalElectroretinographyGlaucomaOcular HypertensionRatsRats, Sprague-DawleyRetinaRetinal Cone Photoreceptor CellsRetinal Ganglion CellsRetinal Horizontal CellsTomography, Optical Coherence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32958209

Citation

Gallego-Ortega, Alejandro, et al. "Functional and Morphological Alterations in a Glaucoma Model of Acute Ocular Hypertension." Progress in Brain Research, vol. 256, no. 1, 2020, pp. 1-29.
Gallego-Ortega A, Norte-Muñoz M, Miralles de Imperial-Ollero JA, et al. Functional and morphological alterations in a glaucoma model of acute ocular hypertension. Prog Brain Res. 2020;256(1):1-29.
Gallego-Ortega, A., Norte-Muñoz, M., Miralles de Imperial-Ollero, J. A., Bernal-Garro, J. M., Valiente-Soriano, F. J., de la Villa Polo, P., Avilés-Trigueros, M., Villegas-Pérez, M. P., & Vidal-Sanz, M. (2020). Functional and morphological alterations in a glaucoma model of acute ocular hypertension. Progress in Brain Research, 256(1), 1-29. https://doi.org/10.1016/bs.pbr.2020.07.003
Gallego-Ortega A, et al. Functional and Morphological Alterations in a Glaucoma Model of Acute Ocular Hypertension. Prog Brain Res. 2020;256(1):1-29. PubMed PMID: 32958209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional and morphological alterations in a glaucoma model of acute ocular hypertension. AU - Gallego-Ortega,Alejandro, AU - Norte-Muñoz,María, AU - Miralles de Imperial-Ollero,Juan Antonio, AU - Bernal-Garro,José Manuel, AU - Valiente-Soriano,Francisco Javier, AU - de la Villa Polo,Pedro, AU - Avilés-Trigueros,Marcelino, AU - Villegas-Pérez,María Paz, AU - Vidal-Sanz,Manuel, Y1 - 2020/08/13/ PY - 2020/9/22/entrez PY - 2020/9/23/pubmed PY - 2021/7/6/medline KW - Acute ocular hypertension KW - Bipolar cells KW - Cones KW - Glaucoma KW - Horizontal cells KW - Rat retina KW - Retinal ganglion cells KW - Transient retinal ischemia SP - 1 EP - 29 JF - Progress in brain research JO - Prog Brain Res VL - 256 IS - 1 N2 - To study short and long-term effects of acute ocular hypertension (AOHT) on inner and outer retinal layers, in adult Sprague-Dawley rats AOHT (87mmHg) was induced for 90min and the retinas were examined longitudinally in vivo with electroretinogram (ERG) recordings and optical coherent tomography (OCT) from 1 to 90 days (d). Ex vivo, the retinas were analyzed for rod (RBC) and cone (CBC) bipolar cells, with antibodies against protein kinase Cα and recoverin, respectively in cross sections, and for cones, horizontal (HZ) and ganglion (RGC) cells with antibodies against arrestin, calbindin and Brn3a, respectively in wholemounts. The inner retina thinned progressively up to 7d with no further changes, while the external retina had a normal thickness until 30d, with a 20% thinning between 30 and 90d. Functionally, the a-wave showed an initial reduction by 24h and a further reduction from 30 to 90d. All other main ERG waves were significantly reduced by 1d without significant recovery by 90d. Radial sections showed a normal population of RBCs but their terminals were reduced. The CBCs showed a progressive decrease with a loss of 56% by 30d. In wholemount retinas, RGCs diminished to 40% by 3d and to 16% by 30d without further loss. Cones diminished to 58% and 35% by 3 and 7d, respectively and further decreased between 30 and 90d. HZs showed normal values throughout the study. In conclusion, AOHT affects both the inner and outer retina, with a more pronounced degeneration of the cone than the rod pathway. SN - 1875-7855 UR - https://www.unboundmedicine.com/medline/citation/32958209/Functional_and_morphological_alterations_in_a_glaucoma_model_of_acute_ocular_hypertension_ DB - PRIME DP - Unbound Medicine ER -