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Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2.J Clin Invest. 2020 12 01; 130(12):6631-6638.JCI
Abstract
BACKGROUND
T cell responses to the common cold coronaviruses have not been well characterized. Preexisting T cell immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported, and a recent study suggested that this immunity was due to cross-recognition of the novel coronavirus by T cells specific for the common cold coronaviruses.METHODS
We used the enzyme-linked immunospot (ELISPOT) assay to characterize the T cell responses against peptide pools derived from the spike protein of 3 common cold coronaviruses (HCoV-229E, HCoV-NL63, and HCoV-OC43) and SARS-CoV-2 in 21 healthy donors (HDs) who were seronegative for SARS-CoV-2 and had no known exposure to the virus. An in vitro expansion culture assay was also used to analyze memory T cell responses.RESULTS
We found responses to the spike protein of the 3 common cold coronaviruses in many of the donors. We then focused on HCoV-NL63 and detected broad T cell responses to the spike protein and identified 22 targeted peptides. Interestingly, only 1 study participant had a significant response to SARS-CoV-2 spike or nucleocapsid protein in the ELISPOT assay. In vitro expansion studies suggested that T cells specific for the HCoV-NL63 spike protein in this individual could also recognize SARS-CoV-2 spike protein peptide pools.CONCLUSIONHDs have circulating T cells specific for the spike proteins of HCoV-NL63, HCoV-229E, and HCoV-OC43. T cell responses to SARS-CoV-2 spike and nucleocapsid proteins were present in only 1 participant and were potentially the result of cross-recognition by T cells specific for the common cold coronaviruses. Further studies are needed to determine whether this cross-recognition influences coronavirus disease 2019 (COVID-19) outcomes.Links
MeSH
Pub Type(s)
Clinical Trial
Journal Article
Research Support, N.I.H., Extramural
Language
eng
PubMed ID
32966269
Citation
Woldemeskel, Bezawit A., et al. "Healthy Donor T Cell Responses to Common Cold Coronaviruses and SARS-CoV-2." The Journal of Clinical Investigation, vol. 130, no. 12, 2020, pp. 6631-6638.
Woldemeskel BA, Kwaa AK, Garliss CC, et al. Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2. J Clin Invest. 2020;130(12):6631-6638.
Woldemeskel, B. A., Kwaa, A. K., Garliss, C. C., Laeyendecker, O., Ray, S. C., & Blankson, J. N. (2020). Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2. The Journal of Clinical Investigation, 130(12), 6631-6638. https://doi.org/10.1172/JCI143120
Woldemeskel BA, et al. Healthy Donor T Cell Responses to Common Cold Coronaviruses and SARS-CoV-2. J Clin Invest. 2020 12 1;130(12):6631-6638. PubMed PMID: 32966269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2.
AU - Woldemeskel,Bezawit A,
AU - Kwaa,Abena K,
AU - Garliss,Caroline C,
AU - Laeyendecker,Oliver,
AU - Ray,Stuart C,
AU - Blankson,Joel N,
PY - 2020/08/07/received
PY - 2020/09/09/accepted
PY - 2020/9/24/pubmed
PY - 2020/12/15/medline
PY - 2020/9/23/entrez
KW - Adaptive immunity
KW - Immunology
KW - Infectious disease
KW - T cells
SP - 6631
EP - 6638
JF - The Journal of clinical investigation
JO - J Clin Invest
VL - 130
IS - 12
N2 - BACKGROUNDT cell responses to the common cold coronaviruses have not been well characterized. Preexisting T cell immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported, and a recent study suggested that this immunity was due to cross-recognition of the novel coronavirus by T cells specific for the common cold coronaviruses.METHODSWe used the enzyme-linked immunospot (ELISPOT) assay to characterize the T cell responses against peptide pools derived from the spike protein of 3 common cold coronaviruses (HCoV-229E, HCoV-NL63, and HCoV-OC43) and SARS-CoV-2 in 21 healthy donors (HDs) who were seronegative for SARS-CoV-2 and had no known exposure to the virus. An in vitro expansion culture assay was also used to analyze memory T cell responses.RESULTSWe found responses to the spike protein of the 3 common cold coronaviruses in many of the donors. We then focused on HCoV-NL63 and detected broad T cell responses to the spike protein and identified 22 targeted peptides. Interestingly, only 1 study participant had a significant response to SARS-CoV-2 spike or nucleocapsid protein in the ELISPOT assay. In vitro expansion studies suggested that T cells specific for the HCoV-NL63 spike protein in this individual could also recognize SARS-CoV-2 spike protein peptide pools.CONCLUSIONHDs have circulating T cells specific for the spike proteins of HCoV-NL63, HCoV-229E, and HCoV-OC43. T cell responses to SARS-CoV-2 spike and nucleocapsid proteins were present in only 1 participant and were potentially the result of cross-recognition by T cells specific for the common cold coronaviruses. Further studies are needed to determine whether this cross-recognition influences coronavirus disease 2019 (COVID-19) outcomes.
SN - 1558-8238
UR - https://www.unboundmedicine.com/medline/citation/32966269/Healthy_donor_T_cell_responses_to_common_cold_coronaviruses_and_SARS_CoV_2_
L2 - https://doi.org/10.1172/JCI143120
DB - PRIME
DP - Unbound Medicine
ER -