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Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity.
J Microbiol. 2020 Oct; 58(10):886-891.JM

Abstract

Various treatments and agents had been reported to inactivate RNA viruses. Of these, thermal inactivation is generally considered an effective and cheap method of sample preparation for downstream assays. The purpose of this study is to establish a safe inactivation method for SARS-CoV-2 without compromising the amount of amplifiable viral genome necessary for clinical diagnoses. In this study, we demonstrate the infectivity and genomic stability of SARSCoV- 2 by thermal inactivation at both 56°C and 65°C. The results substantiate that viable SARS-CoV-2 is readily inactivated when incubated at 56°C for 30 min or at 65°C for 10 min. qRT-PCR of specimens heat-inactivated at 56°C for 30 min or 65°C for 15 min revealed similar genomic RNA stability compared with non-heat inactivated specimens. Further, we demonstrate that 30 min of thermal inactivation at 56°C could inactivate viable viruses from clinical COVID-19 specimens without attenuating the qRT-PCR diagnostic sensitivity. Heat treatment of clinical specimens from COVID-19 patients at 56°C for 30 min or 65°C for 15 min could be a useful method for the inactivation of a highly contagious agent, SARS-CoV-2. Use of this method would reduce the potential for secondary infections in BSL2 conditions during diagnostic procedures. Importantly, infectious virus can be inactivated in clinical specimens without compromising the sensitivity of the diagnostic RT-PCR assay.

Authors+Show Affiliations

College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea. Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea. Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea. Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea. Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, 28644, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea.Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon, 34141, Republic of Korea.College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea. choiki55@chungbuk.ac.kr. Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, 28644, Republic of Korea. choiki55@chungbuk.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32989642

Citation

Kim, Young-Il, et al. "Development of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Thermal Inactivation Method With Preservation of Diagnostic Sensitivity." Journal of Microbiology (Seoul, Korea), vol. 58, no. 10, 2020, pp. 886-891.
Kim YI, Casel MAB, Kim SM, et al. Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity. J Microbiol. 2020;58(10):886-891.
Kim, Y. I., Casel, M. A. B., Kim, S. M., Kim, S. G., Park, S. J., Kim, E. H., Jeong, H. W., Poo, H., & Choi, Y. K. (2020). Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity. Journal of Microbiology (Seoul, Korea), 58(10), 886-891. https://doi.org/10.1007/s12275-020-0335-6
Kim YI, et al. Development of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Thermal Inactivation Method With Preservation of Diagnostic Sensitivity. J Microbiol. 2020;58(10):886-891. PubMed PMID: 32989642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity. AU - Kim,Young-Il, AU - Casel,Mark Anthony B, AU - Kim,Se-Mi, AU - Kim,Seong-Gyu, AU - Park,Su-Jin, AU - Kim,Eun-Ha, AU - Jeong,Hye Won, AU - Poo,Haryoung, AU - Choi,Young Ki, Y1 - 2020/09/29/ PY - 2020/07/01/received PY - 2020/08/19/accepted PY - 2020/08/18/revised PY - 2020/9/29/entrez PY - 2020/9/30/pubmed PY - 2020/10/21/medline KW - COVID-19 KW - RT-PCR KW - SARS-CoV-2 KW - genomic stability KW - heat inactivation SP - 886 EP - 891 JF - Journal of microbiology (Seoul, Korea) JO - J Microbiol VL - 58 IS - 10 N2 - Various treatments and agents had been reported to inactivate RNA viruses. Of these, thermal inactivation is generally considered an effective and cheap method of sample preparation for downstream assays. The purpose of this study is to establish a safe inactivation method for SARS-CoV-2 without compromising the amount of amplifiable viral genome necessary for clinical diagnoses. In this study, we demonstrate the infectivity and genomic stability of SARSCoV- 2 by thermal inactivation at both 56°C and 65°C. The results substantiate that viable SARS-CoV-2 is readily inactivated when incubated at 56°C for 30 min or at 65°C for 10 min. qRT-PCR of specimens heat-inactivated at 56°C for 30 min or 65°C for 15 min revealed similar genomic RNA stability compared with non-heat inactivated specimens. Further, we demonstrate that 30 min of thermal inactivation at 56°C could inactivate viable viruses from clinical COVID-19 specimens without attenuating the qRT-PCR diagnostic sensitivity. Heat treatment of clinical specimens from COVID-19 patients at 56°C for 30 min or 65°C for 15 min could be a useful method for the inactivation of a highly contagious agent, SARS-CoV-2. Use of this method would reduce the potential for secondary infections in BSL2 conditions during diagnostic procedures. Importantly, infectious virus can be inactivated in clinical specimens without compromising the sensitivity of the diagnostic RT-PCR assay. SN - 1976-3794 UR - https://www.unboundmedicine.com/medline/citation/32989642/Development_of_severe_acute_respiratory_syndrome_coronavirus_2__SARS_CoV_2__thermal_inactivation_method_with_preservation_of_diagnostic_sensitivity_ L2 - https://dx.doi.org/10.1007/s12275-020-0335-6 DB - PRIME DP - Unbound Medicine ER -