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Multiplex detection of 14 fentanyl analogues and U-47700 in biological samples: Application to a panel of French hospitalized patients.
Forensic Sci Int. 2020 Dec; 317:110437.FS

Abstract

Synthetic opioids (SO) associated with the recent alarming increase of deaths and intoxications in United States of America and Europe are not detected by the usual first-line opiates drug screening assays. We developed a liquid chromatography tandem mass spectrometry analytical method for the multiplex detection of 14 fentanyl analogues (2-furanylfentanyl, 4-ANPP, 4-methoxybutyrylfentanyl, acrylfentanyl, alfentanil, carfentanil, despropionyl-2-fluorofentanyl, fentanyl, methoxyacetylfentanyl, norfentanyl, ocfentanil, remifentanil, sufentanil and valerylfentanyl) and U-47700 in whole blood and urine samples. The method was validated according to the requirements of ISO 15189. A simple and fast liquid-liquid extraction (LLE) with De-Tox Tube-A was performed leading to better recovery of molecules in urine than in blood samples. Depending on the compound, the limits of detection (LODs) ranged from 0.01 to 0.10 ng/mL and from 0.02 to 0.05 ng/mL in whole blood and urine, respectively. Calibration curves were linear in the range 0.5-50.0 ng/mL and the limit of quantification (LOQ) ranged from 0.10 to 0.40 ng/mL in blood. Internal quality controls at 1 and 40 ng/mL showed intra-day and between-day precision and accuracy bias below 10% in urine and 15% in blood. The method was applied to the screening of 211 urine samples from patients admitted in emergency or addiction departments. The presence of legal fentanyl analogues in 5 urine samples was justified by their therapeutic use as analgesics. Only one patient was concerned by fentanyl misuse and addiction whereas no illegal SO was detected. This study is not in favor of a huge misuse of SO in the Lorraine region.

Authors+Show Affiliations

Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.Pôle Hospitalo-Universitaire du Grand Nancy, Centre Psychothérapique de Nancy, Département d'Addictologie, F-54520 Laxou, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.Université de Lorraine, CHRU-Nancy, Department of Clinical Pharmacology and Toxicology, F-54000, Nancy, France; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France. Electronic address: n.gambier@chru-nancy.fr.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

33007729

Citation

Jung, Jean, et al. "Multiplex Detection of 14 Fentanyl Analogues and U-47700 in Biological Samples: Application to a Panel of French Hospitalized Patients." Forensic Science International, vol. 317, 2020, p. 110437.
Jung J, Kolodziej A, Pape E, et al. Multiplex detection of 14 fentanyl analogues and U-47700 in biological samples: Application to a panel of French hospitalized patients. Forensic Sci Int. 2020;317:110437.
Jung, J., Kolodziej, A., Pape, E., Bisch, M., Javot, L., Gibaja, V., Jouzeau, J. Y., Scala-Bertola, J., & Gambier, N. (2020). Multiplex detection of 14 fentanyl analogues and U-47700 in biological samples: Application to a panel of French hospitalized patients. Forensic Science International, 317, 110437. https://doi.org/10.1016/j.forsciint.2020.110437
Jung J, et al. Multiplex Detection of 14 Fentanyl Analogues and U-47700 in Biological Samples: Application to a Panel of French Hospitalized Patients. Forensic Sci Int. 2020;317:110437. PubMed PMID: 33007729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex detection of 14 fentanyl analogues and U-47700 in biological samples: Application to a panel of French hospitalized patients. AU - Jung,Jean, AU - Kolodziej,Allan, AU - Pape,Elise, AU - Bisch,Michael, AU - Javot,Lucie, AU - Gibaja,Valérie, AU - Jouzeau,Jean-Yves, AU - Scala-Bertola,Julien, AU - Gambier,Nicolas, Y1 - 2020/09/06/ PY - 2020/04/22/received PY - 2020/07/28/revised PY - 2020/07/29/accepted PY - 2020/10/3/pubmed PY - 2021/5/13/medline PY - 2020/10/2/entrez KW - Fentanyl analogues KW - HPLC-MS/MS KW - Misuse KW - Synthetic opioids KW - U-47700 SP - 110437 EP - 110437 JF - Forensic science international JO - Forensic Sci Int VL - 317 N2 - Synthetic opioids (SO) associated with the recent alarming increase of deaths and intoxications in United States of America and Europe are not detected by the usual first-line opiates drug screening assays. We developed a liquid chromatography tandem mass spectrometry analytical method for the multiplex detection of 14 fentanyl analogues (2-furanylfentanyl, 4-ANPP, 4-methoxybutyrylfentanyl, acrylfentanyl, alfentanil, carfentanil, despropionyl-2-fluorofentanyl, fentanyl, methoxyacetylfentanyl, norfentanyl, ocfentanil, remifentanil, sufentanil and valerylfentanyl) and U-47700 in whole blood and urine samples. The method was validated according to the requirements of ISO 15189. A simple and fast liquid-liquid extraction (LLE) with De-Tox Tube-A was performed leading to better recovery of molecules in urine than in blood samples. Depending on the compound, the limits of detection (LODs) ranged from 0.01 to 0.10 ng/mL and from 0.02 to 0.05 ng/mL in whole blood and urine, respectively. Calibration curves were linear in the range 0.5-50.0 ng/mL and the limit of quantification (LOQ) ranged from 0.10 to 0.40 ng/mL in blood. Internal quality controls at 1 and 40 ng/mL showed intra-day and between-day precision and accuracy bias below 10% in urine and 15% in blood. The method was applied to the screening of 211 urine samples from patients admitted in emergency or addiction departments. The presence of legal fentanyl analogues in 5 urine samples was justified by their therapeutic use as analgesics. Only one patient was concerned by fentanyl misuse and addiction whereas no illegal SO was detected. This study is not in favor of a huge misuse of SO in the Lorraine region. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/33007729/Multiplex_detection_of_14_fentanyl_analogues_and_U_47700_in_biological_samples:_Application_to_a_panel_of_French_hospitalized_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(20)30299-1 DB - PRIME DP - Unbound Medicine ER -