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Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair.
Biochem Biophys Res Commun. 2020 12 17; 533(4):899-904.BB

Abstract

Non-homologous end joining (NHEJ) is a highly conserved mechanism of DNA double-stranded break (DSB) repair. Here we utilize a computational protein-protein interaction method to identify human PRKACB as a potential candidate interacting with NHEJ proteins. We show that the deletion of its yeast homolog, TPK1 that codes for the protein kinase A catalytic subunit reduces the efficiency of NHEJ repair of breaks with overhangs and blunt ends in plasmid-based repair assays. Additionally, tpk1Δ mutants showed defects in the repair of chromosomal breaks induced by HO-site specific endonuclease. Our double deletion mutant analyses suggest that TPK1 and YKU80, a key player in NHEJ could function in parallel pathways. Altogether, here we report a novel involvement for TPK1 in NHEJ.

Authors+Show Affiliations

Department of Biology, Carleton University, Ottawa, Ontario, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.Department of Biochemistry, Research and Innovation Centre, University of Regina, Regina, Saskatchewan, Canada.Department of Biology, Carleton University, Ottawa, Ontario, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.Department of Biology, Carleton University, Ottawa, Ontario, Canada.Department of Biochemistry, Research and Innovation Centre, University of Regina, Regina, Saskatchewan, Canada. Electronic address: mohan.babu@uregina.ca.Department of Biology, Carleton University, Ottawa, Ontario, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada. Electronic address: Ashkan.Golshani@carleton.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33008596

Citation

Hooshyar, Mohsen, et al. "Deletion of Yeast TPK1 Reduces the Efficiency of Non-homologous End Joining DNA Repair." Biochemical and Biophysical Research Communications, vol. 533, no. 4, 2020, pp. 899-904.
Hooshyar M, Jessulat M, Burnside D, et al. Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair. Biochem Biophys Res Commun. 2020;533(4):899-904.
Hooshyar, M., Jessulat, M., Burnside, D., Kluew, A., Babu, M., & Golshani, A. (2020). Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair. Biochemical and Biophysical Research Communications, 533(4), 899-904. https://doi.org/10.1016/j.bbrc.2020.09.083
Hooshyar M, et al. Deletion of Yeast TPK1 Reduces the Efficiency of Non-homologous End Joining DNA Repair. Biochem Biophys Res Commun. 2020 12 17;533(4):899-904. PubMed PMID: 33008596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair. AU - Hooshyar,Mohsen, AU - Jessulat,Matthew, AU - Burnside,Daniel, AU - Kluew,Anna, AU - Babu,Mohan, AU - Golshani,Ashkan, Y1 - 2020/09/30/ PY - 2020/09/18/received PY - 2020/09/20/accepted PY - 2020/10/4/pubmed PY - 2021/3/30/medline PY - 2020/10/3/entrez KW - DNA double Stranded breaks KW - DNA repair KW - Non-homologous end joining KW - TPK1 KW - YKU80 KW - Yeast SP - 899 EP - 904 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 533 IS - 4 N2 - Non-homologous end joining (NHEJ) is a highly conserved mechanism of DNA double-stranded break (DSB) repair. Here we utilize a computational protein-protein interaction method to identify human PRKACB as a potential candidate interacting with NHEJ proteins. We show that the deletion of its yeast homolog, TPK1 that codes for the protein kinase A catalytic subunit reduces the efficiency of NHEJ repair of breaks with overhangs and blunt ends in plasmid-based repair assays. Additionally, tpk1Δ mutants showed defects in the repair of chromosomal breaks induced by HO-site specific endonuclease. Our double deletion mutant analyses suggest that TPK1 and YKU80, a key player in NHEJ could function in parallel pathways. Altogether, here we report a novel involvement for TPK1 in NHEJ. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/33008596/Deletion_of_yeast_TPK1_reduces_the_efficiency_of_non_homologous_end_joining_DNA_repair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(20)31830-1 DB - PRIME DP - Unbound Medicine ER -