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Diversity and Bioactivity of Endophytes From Angelica sinensis in China.
Front Microbiol. 2020; 11:1489.FM

Abstract

Plant seeds are not merely reproductive organs, they are also carriers of microorganism, particularly, inherent and non-invasive characteristic endophytes in host plant. Therefore, in this study, the endophytic diversity of Angelica seeds was studied and compared with endophytes isolated from healthy leaves, stems, roots, and seeds of A. sinensis using 20 different media. The metabolites of endophytic strains were evaluated with six different methods for their antioxidant activity and the paper disc diffusion method for antimicrobial activities. As a result, 226 endophytes were isolated. Compared with the biodiversity and abundance of uncultured fungi from Angelica seed, the result showed that the most frequent endophytic fungi were Alternaria sp. as seen in artificial media; moreover, compared with artificial media, the pathogenic fungi, including Fusarium sp. and Pseudallescheria sp., were not found from the Angelica seed, the results suggested it may not be inherent endophytes in plants. In addition, bacteria from seven phyla were identified by high-throughput sequencing, while five phyla of endophytic bacteria were not isolated on artificial media including Proteobacteria, Actinobacteria, Bacteroidetes, Microgenomates, and Saccharibacteria. Furthermore, the sample JH-4 mycelium displayed the best antioxidant activity, and the active constituent may be a flavonoid as determined by total phenol and flavonoid content. Moreover, YH-12-1 mycelium had strong inhibitory activity against the five tested strains and the minimum inhibitory concentration (MIC) against Pseudomonas aeruginosa and Streptococcus pneumoniae was found to be 25 μg/mL. Our results confirm that plant endophytes are rich in biodiversity and contain important resource of many uncultured microorganisms.

Authors+Show Affiliations

School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.School of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, China. Key Laboratory of Screening and Processing in New Tibetan Medicine of Gansu Province, Gansu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33013716

Citation

Zhang, Xin-Guo, et al. "Diversity and Bioactivity of Endophytes From Angelica Sinensis in China." Frontiers in Microbiology, vol. 11, 2020, p. 1489.
Zhang XG, Guo SJ, Wang WN, et al. Diversity and Bioactivity of Endophytes From Angelica sinensis in China. Front Microbiol. 2020;11:1489.
Zhang, X. G., Guo, S. J., Wang, W. N., Wei, G. X., Ma, G. Y., & Ma, X. D. (2020). Diversity and Bioactivity of Endophytes From Angelica sinensis in China. Frontiers in Microbiology, 11, 1489. https://doi.org/10.3389/fmicb.2020.01489
Zhang XG, et al. Diversity and Bioactivity of Endophytes From Angelica Sinensis in China. Front Microbiol. 2020;11:1489. PubMed PMID: 33013716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and Bioactivity of Endophytes From Angelica sinensis in China. AU - Zhang,Xin-Guo, AU - Guo,Si-Jia, AU - Wang,Wen-Na, AU - Wei,Guo-Xing, AU - Ma,Guo-Yan, AU - Ma,Xiao-Di, Y1 - 2020/08/18/ PY - 2019/12/24/received PY - 2020/06/08/accepted PY - 2020/10/5/entrez PY - 2020/10/6/pubmed PY - 2020/10/6/medline KW - Angelica sinensis KW - antimicrobial activity KW - antioxidant activity KW - diversity KW - endophytes KW - secondary metabolite SP - 1489 EP - 1489 JF - Frontiers in microbiology JO - Front Microbiol VL - 11 N2 - Plant seeds are not merely reproductive organs, they are also carriers of microorganism, particularly, inherent and non-invasive characteristic endophytes in host plant. Therefore, in this study, the endophytic diversity of Angelica seeds was studied and compared with endophytes isolated from healthy leaves, stems, roots, and seeds of A. sinensis using 20 different media. The metabolites of endophytic strains were evaluated with six different methods for their antioxidant activity and the paper disc diffusion method for antimicrobial activities. As a result, 226 endophytes were isolated. Compared with the biodiversity and abundance of uncultured fungi from Angelica seed, the result showed that the most frequent endophytic fungi were Alternaria sp. as seen in artificial media; moreover, compared with artificial media, the pathogenic fungi, including Fusarium sp. and Pseudallescheria sp., were not found from the Angelica seed, the results suggested it may not be inherent endophytes in plants. In addition, bacteria from seven phyla were identified by high-throughput sequencing, while five phyla of endophytic bacteria were not isolated on artificial media including Proteobacteria, Actinobacteria, Bacteroidetes, Microgenomates, and Saccharibacteria. Furthermore, the sample JH-4 mycelium displayed the best antioxidant activity, and the active constituent may be a flavonoid as determined by total phenol and flavonoid content. Moreover, YH-12-1 mycelium had strong inhibitory activity against the five tested strains and the minimum inhibitory concentration (MIC) against Pseudomonas aeruginosa and Streptococcus pneumoniae was found to be 25 μg/mL. Our results confirm that plant endophytes are rich in biodiversity and contain important resource of many uncultured microorganisms. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/33013716/Diversity_and_Bioactivity_of_Endophytes_From_Angelica_sinensis_in_China. L2 - https://doi.org/10.3389/fmicb.2020.01489 DB - PRIME DP - Unbound Medicine ER -
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