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Quantification of Classic, Prescription, and Synthetic Opioids in Hair by LC-MS-MS.
J Anal Toxicol. 2020 Oct 07 [Online ahead of print]JA

Abstract

The current use and misuse of synthetic and prescription opioids in the US has reached epidemic status. According to the US Department of Health and Human Services, every day more than 130 people in the US die after overdosing on opioids, and 2.1 million had an opioid use disorder in 2018. Hair is becoming an alternative matrix of increasing interest in forensic toxicology to investigate drug use and abuse patterns due to its long window of detection. The focus of this project was to develop and validate a method that simultaneously detects and quantifies 27 classic, prescription and synthetic opioids in hair by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Hair samples were decontaminated and pulverized in a bead mill. Twenty-five milligrams of hair powder were incubated in a buffer overnight. Mixed mode cation exchange solid phase extraction was carried out before undergoing reversed-phase chromatographic separation, successfully resolving isobaric opioids. We used two multiple-reaction monitoring transitions in positive mode to identify each analyte. The linearity range was 1-500 pg/mg for fentanyl and synthetic opioids, and 10-500 pg/mg for prescription and classic opioids. Imprecision was <17.5% and bias ranged from -13.6 to 12.0%. Majority of compounds showed extraction efficiency >50%, and ion suppression from -89.2 to -26.6% (CV<19%, n = 10). This method was applied to 64 authentic cases, identifying 13 compounds from our panel. A sensitive and specific method was developed for the identification and quantification of 27 classic, prescription and synthetic opioids in hair by LC-MS-MS.

Authors+Show Affiliations

Department of Sciences, John Jay College of Criminal Justice, City University of New York, NY, USA.Center for Forensic Hair Analytics, Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.Center for Forensic Hair Analytics, Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.Servizo de Toxicoloxía, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.Servizo de Toxicoloxía, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.Department of Sciences, John Jay College of Criminal Justice, City University of New York, NY, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33027514

Citation

Platosz, Natalia A., et al. "Quantification of Classic, Prescription, and Synthetic Opioids in Hair By LC-MS-MS." Journal of Analytical Toxicology, 2020.
Platosz NA, Binz TM, Baumgartner MR, et al. Quantification of Classic, Prescription, and Synthetic Opioids in Hair by LC-MS-MS. J Anal Toxicol. 2020.
Platosz, N. A., Binz, T. M., Baumgartner, M. R., Lendoiro, E., de Castro, A., & Concheiro, M. (2020). Quantification of Classic, Prescription, and Synthetic Opioids in Hair by LC-MS-MS. Journal of Analytical Toxicology. https://doi.org/10.1093/jat/bkaa152
Platosz NA, et al. Quantification of Classic, Prescription, and Synthetic Opioids in Hair By LC-MS-MS. J Anal Toxicol. 2020 Oct 7; PubMed PMID: 33027514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of Classic, Prescription, and Synthetic Opioids in Hair by LC-MS-MS. AU - Platosz,Natalia A, AU - Binz,Tina M, AU - Baumgartner,Markus R, AU - Lendoiro,Elena, AU - de Castro,Ana, AU - Concheiro,Marta, Y1 - 2020/10/07/ PY - 2020/06/08/received PY - 2020/09/01/revised PY - 2020/10/05/accepted PY - 2020/10/7/entrez PY - 2020/10/8/pubmed PY - 2020/10/8/medline JF - Journal of analytical toxicology JO - J Anal Toxicol N2 - The current use and misuse of synthetic and prescription opioids in the US has reached epidemic status. According to the US Department of Health and Human Services, every day more than 130 people in the US die after overdosing on opioids, and 2.1 million had an opioid use disorder in 2018. Hair is becoming an alternative matrix of increasing interest in forensic toxicology to investigate drug use and abuse patterns due to its long window of detection. The focus of this project was to develop and validate a method that simultaneously detects and quantifies 27 classic, prescription and synthetic opioids in hair by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Hair samples were decontaminated and pulverized in a bead mill. Twenty-five milligrams of hair powder were incubated in a buffer overnight. Mixed mode cation exchange solid phase extraction was carried out before undergoing reversed-phase chromatographic separation, successfully resolving isobaric opioids. We used two multiple-reaction monitoring transitions in positive mode to identify each analyte. The linearity range was 1-500 pg/mg for fentanyl and synthetic opioids, and 10-500 pg/mg for prescription and classic opioids. Imprecision was <17.5% and bias ranged from -13.6 to 12.0%. Majority of compounds showed extraction efficiency >50%, and ion suppression from -89.2 to -26.6% (CV<19%, n = 10). This method was applied to 64 authentic cases, identifying 13 compounds from our panel. A sensitive and specific method was developed for the identification and quantification of 27 classic, prescription and synthetic opioids in hair by LC-MS-MS. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/33027514/Quantification_of_Classic_Prescription_and_Synthetic_Opioids_in_Hair_by_LC_MS_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkaa152 DB - PRIME DP - Unbound Medicine ER -
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