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SARS-CoV-2 infection, disease and transmission in domestic cats.
Emerg Microbes Infect. 2020 Dec; 9(1):2322-2332.EM

Abstract

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infection, disease and transmission in domestic cats. Cats were challenged with SARS-CoV-2 via intranasal and oral routes. One day post challenge (DPC), two sentinel cats were introduced. Animals were monitored for clinical signs, clinicopathological abnormalities and viral shedding. Postmortem examinations were performed at 4, 7 and 21 DPC. Viral RNA was not detected in blood but transiently in nasal, oropharyngeal and rectal swabs and bronchoalveolar lavage fluid as well as various tissues. Tracheobronchoadenitis of submucosal glands with the presence of viral RNA and antigen was observed in airways of the infected cats. Serology showed that both, principals and sentinels, developed antibodies to SARS-CoV-2. All animals were clinically asymptomatic during the course of the study and capable of transmitting SARS-CoV-2 to sentinels. The results of this study are critical for understanding the clinical course of SARS-CoV-2 in a naturally susceptible host species, and for risk assessment.

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Authors+Show Affiliations

Gaudreault NN
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Trujillo JD
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Carossino M
Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Meekins DA
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Morozov I
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Madden DW
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Indran SV
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Bold D
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Balaraman V
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Kwon T
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Artiaga BL
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Cool K
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
García-Sastre A
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA. The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Ma W
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Wilson WC
Arthropod Borne Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS, USA.
Henningson J
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Balasuriya UBR
Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Richt JA
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.

MeSH

AnimalsAntibodies, NeutralizingAntibodies, ViralBetacoronavirusBronchoalveolar Lavage FluidCOVID-19Cat DiseasesCatsCell LineChlorocebus aethiopsCoronavirus InfectionsDisease SusceptibilityMalePandemicsPneumonia, ViralRNA, ViralSARS-CoV-2Vero CellsVirus Replication

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33028154

Citation

Gaudreault, Natasha N., et al. "SARS-CoV-2 Infection, Disease and Transmission in Domestic Cats." Emerging Microbes & Infections, vol. 9, no. 1, 2020, pp. 2322-2332.
Gaudreault NN, Trujillo JD, Carossino M, et al. SARS-CoV-2 infection, disease and transmission in domestic cats. Emerg Microbes Infect. 2020;9(1):2322-2332.
Gaudreault, N. N., Trujillo, J. D., Carossino, M., Meekins, D. A., Morozov, I., Madden, D. W., Indran, S. V., Bold, D., Balaraman, V., Kwon, T., Artiaga, B. L., Cool, K., García-Sastre, A., Ma, W., Wilson, W. C., Henningson, J., Balasuriya, U. B. R., & Richt, J. A. (2020). SARS-CoV-2 infection, disease and transmission in domestic cats. Emerging Microbes & Infections, 9(1), 2322-2332. https://doi.org/10.1080/22221751.2020.1833687
Gaudreault NN, et al. SARS-CoV-2 Infection, Disease and Transmission in Domestic Cats. Emerg Microbes Infect. 2020;9(1):2322-2332. PubMed PMID: 33028154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 infection, disease and transmission in domestic cats. AU - Gaudreault,Natasha N, AU - Trujillo,Jessie D, AU - Carossino,Mariano, AU - Meekins,David A, AU - Morozov,Igor, AU - Madden,Daniel W, AU - Indran,Sabarish V, AU - Bold,Dashzeveg, AU - Balaraman,Velmurugan, AU - Kwon,Taeyong, AU - Artiaga,Bianca Libanori, AU - Cool,Konner, AU - García-Sastre,Adolfo, AU - Ma,Wenjun, AU - Wilson,William C, AU - Henningson,Jamie, AU - Balasuriya,Udeni B R, AU - Richt,Juergen A, PY - 2020/10/9/pubmed PY - 2020/11/3/medline PY - 2020/10/8/entrez KW - COVID-19 KW - SARS-CoV-2 KW - cats KW - felines KW - susceptibility KW - transmission SP - 2322 EP - 2332 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 9 IS - 1 N2 - Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infection, disease and transmission in domestic cats. Cats were challenged with SARS-CoV-2 via intranasal and oral routes. One day post challenge (DPC), two sentinel cats were introduced. Animals were monitored for clinical signs, clinicopathological abnormalities and viral shedding. Postmortem examinations were performed at 4, 7 and 21 DPC. Viral RNA was not detected in blood but transiently in nasal, oropharyngeal and rectal swabs and bronchoalveolar lavage fluid as well as various tissues. Tracheobronchoadenitis of submucosal glands with the presence of viral RNA and antigen was observed in airways of the infected cats. Serology showed that both, principals and sentinels, developed antibodies to SARS-CoV-2. All animals were clinically asymptomatic during the course of the study and capable of transmitting SARS-CoV-2 to sentinels. The results of this study are critical for understanding the clinical course of SARS-CoV-2 in a naturally susceptible host species, and for risk assessment. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/33028154/SARS_CoV_2_infection_disease_and_transmission_in_domestic_cats_ DB - PRIME DP - Unbound Medicine ER -