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Genome-wide association study identifies QTL for thousand grain weight in winter wheat under normal- and late-sown stressed environments.
Theor Appl Genet. 2021 Jan; 134(1):143-157.TA

Abstract

KEY MESSAGE

GWAS identified stable loci for TGW and stress tolerance in winter wheat based on two sowing conditions, which will provide opportunities for developing new cultivars with high yield and yield stability. Wheat is an important food crop widely cultivated in the world. Breeding new varieties with high yields and superior adaptability is the main goal of modern wheat breeding program. In order to determine the marker-trait associations (MATs), a set of 688 diverse winter wheat accessions were subjected to genome-wide association study (GWAS) using the wheat 90K array. Field trials under normal-sown (NS) and late-sown (LS) conditions were conducted for thousand grain weight (TGW) and stress susceptibility index (SSI) at three different sites across two consecutive years. A total of 179 (NS) and 158 (LS) MATs corresponded with TGW; of these, 16 and 6 stable MATs for TGWNS and TGWLS were identified on chromosomes 1B, 2B, 3A, 3B, 5A, 5B, 5D, 6B, and 7D across at least three environments. Notably, a QTL hot spot controlling TGW under NS and LS conditions was found on chromosome 5A (140-142 cM). Moreover, 8 of 228 stable MATs on chromosomes 4B, 5A, and 5D for SSI were detected. A haplotype block associated with TGW and SSI was located on chromosome 5A at 91 cM, nearby the vernalization gene VRN-A1. Additionally, analysis of wheat varieties from the different eras revealed that the grain weight and stress tolerance are not improved concurrently. Overall, our results provide promising alleles controlling grain weight and stress tolerance (particularly for thermotolerance) for wheat breeders, which can be used in marker-assisted selection for improving grain yield and yield stability in wheat.

Authors+Show Affiliations

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.Hebei Crop Genetic Breeding Laboratory, Institute of Cereal and Oil Crops of Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050035, China.Hebei Crop Genetic Breeding Laboratory, Institute of Cereal and Oil Crops of Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050035, China.Agronomy College, Northwest A&F University, Yangling, 712100, Shaanxi, China.Agronomy College, Northwest A&F University, Yangling, 712100, Shaanxi, China.Institute of Wheat, Shanxi Academy of Agricultural Sciences, Linfen, 041000, China.Institute of Wheat, Shanxi Academy of Agricultural Sciences, Linfen, 041000, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. penghuiru@cau.edu.cn.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. qxsun@cau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33030571

Citation

Wang, Xiaobo, et al. "Genome-wide Association Study Identifies QTL for Thousand Grain Weight in Winter Wheat Under Normal- and Late-sown Stressed Environments." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 1, 2021, pp. 143-157.
Wang X, Guan P, Xin M, et al. Genome-wide association study identifies QTL for thousand grain weight in winter wheat under normal- and late-sown stressed environments. Theor Appl Genet. 2021;134(1):143-157.
Wang, X., Guan, P., Xin, M., Wang, Y., Chen, X., Zhao, A., Liu, M., Li, H., Zhang, M., Lu, L., Zhang, J., Ni, Z., Yao, Y., Hu, Z., Peng, H., & Sun, Q. (2021). Genome-wide association study identifies QTL for thousand grain weight in winter wheat under normal- and late-sown stressed environments. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(1), 143-157. https://doi.org/10.1007/s00122-020-03687-w
Wang X, et al. Genome-wide Association Study Identifies QTL for Thousand Grain Weight in Winter Wheat Under Normal- and Late-sown Stressed Environments. Theor Appl Genet. 2021;134(1):143-157. PubMed PMID: 33030571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide association study identifies QTL for thousand grain weight in winter wheat under normal- and late-sown stressed environments. AU - Wang,Xiaobo, AU - Guan,Panfeng, AU - Xin,Mingming, AU - Wang,Yongfa, AU - Chen,Xiyong, AU - Zhao,Aiju, AU - Liu,Manshuang, AU - Li,Hongxia, AU - Zhang,Mingyi, AU - Lu,Lahu, AU - Zhang,Jinbo, AU - Ni,Zhongfu, AU - Yao,Yingyin, AU - Hu,Zhaorong, AU - Peng,Huiru, AU - Sun,Qixin, Y1 - 2020/10/08/ PY - 2020/01/08/received PY - 2020/09/16/accepted PY - 2020/10/9/pubmed PY - 2021/6/11/medline PY - 2020/10/8/entrez SP - 143 EP - 157 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 1 N2 - KEY MESSAGE: GWAS identified stable loci for TGW and stress tolerance in winter wheat based on two sowing conditions, which will provide opportunities for developing new cultivars with high yield and yield stability. Wheat is an important food crop widely cultivated in the world. Breeding new varieties with high yields and superior adaptability is the main goal of modern wheat breeding program. In order to determine the marker-trait associations (MATs), a set of 688 diverse winter wheat accessions were subjected to genome-wide association study (GWAS) using the wheat 90K array. Field trials under normal-sown (NS) and late-sown (LS) conditions were conducted for thousand grain weight (TGW) and stress susceptibility index (SSI) at three different sites across two consecutive years. A total of 179 (NS) and 158 (LS) MATs corresponded with TGW; of these, 16 and 6 stable MATs for TGWNS and TGWLS were identified on chromosomes 1B, 2B, 3A, 3B, 5A, 5B, 5D, 6B, and 7D across at least three environments. Notably, a QTL hot spot controlling TGW under NS and LS conditions was found on chromosome 5A (140-142 cM). Moreover, 8 of 228 stable MATs on chromosomes 4B, 5A, and 5D for SSI were detected. A haplotype block associated with TGW and SSI was located on chromosome 5A at 91 cM, nearby the vernalization gene VRN-A1. Additionally, analysis of wheat varieties from the different eras revealed that the grain weight and stress tolerance are not improved concurrently. Overall, our results provide promising alleles controlling grain weight and stress tolerance (particularly for thermotolerance) for wheat breeders, which can be used in marker-assisted selection for improving grain yield and yield stability in wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/33030571/Genome_wide_association_study_identifies_QTL_for_thousand_grain_weight_in_winter_wheat_under_normal__and_late_sown_stressed_environments_ L2 - https://dx.doi.org/10.1007/s00122-020-03687-w DB - PRIME DP - Unbound Medicine ER -