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Detection of SARS-CoV-2 in the tears and conjunctival secretions of Coronavirus disease 2019 patients.
J Infect Dev Ctries. 2020 09 30; 14(9):977-981.JI

Abstract

INTRODUCTION

Current studies suggest that tears and conjunctival secretions may be an important transmission route in coronavirus disease 2019 (COVID-19). The study aims to evaluate the presence of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) virus in tears and conjunctival secretion of patients with COVID-19.

METHODOLOGY

A prospective interventional case series study was performed, and 32 patients with COVID-19 were selected at the Pamukkale University Hospital from 15 to 22 May 2020. The tear and conjunctival samples were collected by a conjunctival swab. Each specimen was sent to the laboratory for reverse transcription-polymerase chain reaction (RT-PCR) analyses. To avoid cross-infection, gloves and personal protective equipment were changed after collecting each sample.

RESULTS

32 patients (18 male, 14 female) with Covid-19 were included in this cross-sectional study. The average age of the patients was 52.81 ± 16.76 years. By the time of the first collection of conjunctival-tear samples, the mean time of the onset of complaints was 6.84 ± 6.81 (1-35) days. Tear-conjunctival samples from 5 patients (16%) without conjunctivitis yielded positive PCR results, 3 of whom had positive and 2 negative nasopharyngeal PCR results.

CONCLUSIONS

Five of 32 patients (16 %) without conjunctivitis or any eye symptoms had viral RNA in their tear-conjunctival samples. The possibility of transmission via tears and conjunctival secretions should be recognized even in the absence of conjunctivitis or other ocular manifestations.

Authors+Show Affiliations

Ophthalmology Department, Pamukkale University, Denizli, Turkey. hsynkaya@gmail.com.Department of Medical Microbiology, Pamukkale University, Denizli, Turkey. ahmetsuna@msn.com.Department of Medical Microbiology, Pamukkale University, Denizli, Turkey. dr.mokul@gmail.com.Department of Infectious Diseases and Clinical Microbiology, Pamukkale University, Denizli, Turkey. drtugba82@gmail.com.Department of Internal Medicine, Pamukkale University, Denizli, Turkey. ishakbudak@gmail.com.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33031084

Citation

Kaya, Hüseyin, et al. "Detection of SARS-CoV-2 in the Tears and Conjunctival Secretions of Coronavirus Disease 2019 Patients." Journal of Infection in Developing Countries, vol. 14, no. 9, 2020, pp. 977-981.
Kaya H, Çalışkan A, Okul M, et al. Detection of SARS-CoV-2 in the tears and conjunctival secretions of Coronavirus disease 2019 patients. J Infect Dev Ctries. 2020;14(9):977-981.
Kaya, H., Çalışkan, A., Okul, M., Sarı, T., & Akbudak, İ. H. (2020). Detection of SARS-CoV-2 in the tears and conjunctival secretions of Coronavirus disease 2019 patients. Journal of Infection in Developing Countries, 14(9), 977-981. https://doi.org/10.3855/jidc.13224
Kaya H, et al. Detection of SARS-CoV-2 in the Tears and Conjunctival Secretions of Coronavirus Disease 2019 Patients. J Infect Dev Ctries. 2020 09 30;14(9):977-981. PubMed PMID: 33031084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SARS-CoV-2 in the tears and conjunctival secretions of Coronavirus disease 2019 patients. AU - Kaya,Hüseyin, AU - Çalışkan,Ahmet, AU - Okul,Mehmet, AU - Sarı,Tuğba, AU - Akbudak,İsmail Hakkı, Y1 - 2020/09/30/ PY - 2020/06/08/received PY - 2020/08/30/accepted PY - 2020/10/8/entrez PY - 2020/10/9/pubmed PY - 2020/10/27/medline KW - COVID-19 KW - SARS-CoV-2 KW - conjunctiva KW - tears SP - 977 EP - 981 JF - Journal of infection in developing countries JO - J Infect Dev Ctries VL - 14 IS - 9 N2 - INTRODUCTION: Current studies suggest that tears and conjunctival secretions may be an important transmission route in coronavirus disease 2019 (COVID-19). The study aims to evaluate the presence of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) virus in tears and conjunctival secretion of patients with COVID-19. METHODOLOGY: A prospective interventional case series study was performed, and 32 patients with COVID-19 were selected at the Pamukkale University Hospital from 15 to 22 May 2020. The tear and conjunctival samples were collected by a conjunctival swab. Each specimen was sent to the laboratory for reverse transcription-polymerase chain reaction (RT-PCR) analyses. To avoid cross-infection, gloves and personal protective equipment were changed after collecting each sample. RESULTS: 32 patients (18 male, 14 female) with Covid-19 were included in this cross-sectional study. The average age of the patients was 52.81 ± 16.76 years. By the time of the first collection of conjunctival-tear samples, the mean time of the onset of complaints was 6.84 ± 6.81 (1-35) days. Tear-conjunctival samples from 5 patients (16%) without conjunctivitis yielded positive PCR results, 3 of whom had positive and 2 negative nasopharyngeal PCR results. CONCLUSIONS: Five of 32 patients (16 %) without conjunctivitis or any eye symptoms had viral RNA in their tear-conjunctival samples. The possibility of transmission via tears and conjunctival secretions should be recognized even in the absence of conjunctivitis or other ocular manifestations. SN - 1972-2680 UR - https://www.unboundmedicine.com/medline/citation/33031084/Detection_of_SARS_CoV_2_in_the_tears_and_conjunctival_secretions_of_Coronavirus_disease_2019_patients_ L2 - http://www.jidc.org/index.php/journal/article/view/33031084 DB - PRIME DP - Unbound Medicine ER -