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Differential expression and significance of miRNAs in plasma extracellular vesicles of patients with Parkinson's disease.
Int J Neurosci. 2022 Jul; 132(7):673-688.IJ

Abstract

OBJECTIVE

To study the feasibility of plasma extracellular vesicles (EVs) miRNAs as diagnostic biomarkers for Parkinson's disease (PD).

METHODS

Plasma EVs were isolated from 30 PD patients and 30 age- and sex-matched healthy controls. Plasma EVs miRNAs were analysed by qRT-PCR. SH-SY5Y cells were induced by different concentrations of 1-Methyl-4-phenil-pyridinium (MPP+) to obtain PD cellular model. The levels of miRNAs and α-synuclein (α-syn) in PD cellular model were analysed by qRT-PCR and Western blot. Receiver operating characteristic (ROC) curve analysis was performed to determine the diagnostic usefulness of the miRNAs in plasma EVs for PD. The gene ontology (GO) and KEGG pathways of the target genes of miRNAs were analysed by softwares.

RESULTS

The level of hsa-miR-30c-2-3p in plasma EVs was significantly higher in PD patients than that in controls, and the levels of hsa-miR-15b-5p, hsa-miR-138-5p, hsa-miR-338-3p, hsa-miR-106b-3p and hsa-miR-431-5p in plasma EVs were lower in PD patients than that in controls. When compared with the control group, the area under the curve (AUC) values for hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p, hsa-miR-431-5p, hsa-miR-338-3p and hsa-miR-106b-3p were all greater than 0.6. The target genes of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-338-3p were enriched in dopaminergic synapse and PD pathway.

CONCLUSIONS

The hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p, hsa-miR-106b-3p, hsa-miR-338-3p and hsa-miR-431-5p may be used as potential biomarkers for the diagnosis of PD, and the combined diagnostic accuracy of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-106b-3p was better. The target genes of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-338-3p may regulate the expression of dopamine by dopaminergic synapse and PD pathway.HighlightsIsolation and identification of plasma EVs.The miRNAs in plasma EVs may be used as potential biomarkers for the diagnosis of PD.When SH-SY5Y cells were induced by different concentrations of MPP+, the levels of miRNAs and α-syn changed gradually.The target genes of miRNAs were enriched in dopaminergic synapse and PD pathway.The target genes of miRNAs may regulate the expression of dopamine.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xie S
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China. Department of Neurosurgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, PR China.
Niu W
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China. Department of Neurosurgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, PR China.
Xu F
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China. Department of Neurosurgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, PR China.
Wang Y
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China.
Hu S
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China.
Niu C
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, PR China. Anhui Provincial Key Laboratory of Brain Function and Brain Disease, Hefei, PR China. Department of Neurosurgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, PR China.

MeSH

BiomarkersDopamineExtracellular VesiclesHumansMicroRNAsNeuroblastomaParkinson Disease

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33045885

Citation

Xie, Shishuai, et al. "Differential Expression and Significance of miRNAs in Plasma Extracellular Vesicles of Patients With Parkinson's Disease." The International Journal of Neuroscience, vol. 132, no. 7, 2022, pp. 673-688.
Xie S, Niu W, Xu F, et al. Differential expression and significance of miRNAs in plasma extracellular vesicles of patients with Parkinson's disease. Int J Neurosci. 2022;132(7):673-688.
Xie, S., Niu, W., Xu, F., Wang, Y., Hu, S., & Niu, C. (2022). Differential expression and significance of miRNAs in plasma extracellular vesicles of patients with Parkinson's disease. The International Journal of Neuroscience, 132(7), 673-688. https://doi.org/10.1080/00207454.2020.1835899
Xie S, et al. Differential Expression and Significance of miRNAs in Plasma Extracellular Vesicles of Patients With Parkinson's Disease. Int J Neurosci. 2022;132(7):673-688. PubMed PMID: 33045885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression and significance of miRNAs in plasma extracellular vesicles of patients with Parkinson's disease. AU - Xie,Shishuai, AU - Niu,Wanxiang, AU - Xu,Feng, AU - Wang,Yuping, AU - Hu,Shanshan, AU - Niu,Chaoshi, Y1 - 2020/10/26/ PY - 2020/10/14/pubmed PY - 2022/7/19/medline PY - 2020/10/13/entrez KW - KEGG pathway KW - Parkinson’s disease KW - extracellular vesicles KW - gene ontology KW - miRNAs KW - α-synuclein SP - 673 EP - 688 JF - The International journal of neuroscience JO - Int J Neurosci VL - 132 IS - 7 N2 - OBJECTIVE: To study the feasibility of plasma extracellular vesicles (EVs) miRNAs as diagnostic biomarkers for Parkinson's disease (PD). METHODS: Plasma EVs were isolated from 30 PD patients and 30 age- and sex-matched healthy controls. Plasma EVs miRNAs were analysed by qRT-PCR. SH-SY5Y cells were induced by different concentrations of 1-Methyl-4-phenil-pyridinium (MPP+) to obtain PD cellular model. The levels of miRNAs and α-synuclein (α-syn) in PD cellular model were analysed by qRT-PCR and Western blot. Receiver operating characteristic (ROC) curve analysis was performed to determine the diagnostic usefulness of the miRNAs in plasma EVs for PD. The gene ontology (GO) and KEGG pathways of the target genes of miRNAs were analysed by softwares. RESULTS: The level of hsa-miR-30c-2-3p in plasma EVs was significantly higher in PD patients than that in controls, and the levels of hsa-miR-15b-5p, hsa-miR-138-5p, hsa-miR-338-3p, hsa-miR-106b-3p and hsa-miR-431-5p in plasma EVs were lower in PD patients than that in controls. When compared with the control group, the area under the curve (AUC) values for hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p, hsa-miR-431-5p, hsa-miR-338-3p and hsa-miR-106b-3p were all greater than 0.6. The target genes of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-338-3p were enriched in dopaminergic synapse and PD pathway. CONCLUSIONS: The hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p, hsa-miR-106b-3p, hsa-miR-338-3p and hsa-miR-431-5p may be used as potential biomarkers for the diagnosis of PD, and the combined diagnostic accuracy of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-106b-3p was better. The target genes of hsa-miR-15b-5p, hsa-miR-30c-2-3p, hsa-miR-138-5p and hsa-miR-338-3p may regulate the expression of dopamine by dopaminergic synapse and PD pathway.HighlightsIsolation and identification of plasma EVs.The miRNAs in plasma EVs may be used as potential biomarkers for the diagnosis of PD.When SH-SY5Y cells were induced by different concentrations of MPP+, the levels of miRNAs and α-syn changed gradually.The target genes of miRNAs were enriched in dopaminergic synapse and PD pathway.The target genes of miRNAs may regulate the expression of dopamine. SN - 1563-5279 UR - https://www.unboundmedicine.com/medline/citation/33045885/Differential_expression_and_significance_of_miRNAs_in_plasma_extracellular_vesicles_of_patients_with_Parkinson's_disease_ DB - PRIME DP - Unbound Medicine ER -