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Identification and cross-validation of genetic loci conferring resistance to Septoria nodorum blotch using a German multi-founder winter wheat population.
Theor Appl Genet. 2021 Jan; 134(1):125-142.TA

Abstract

KEY MESSAGE

We identified allelic variation at two major loci, QSnb.nmbu-2A.1 and QSnb.nmbu-5A.1, showing consistent and additive effects on SNB field resistance. Validation of QSnb.nmbu-2A.1 across genetic backgrounds further highlights its usefulness for marker-assisted selection. Septoria nodorum blotch (SNB) is a disease of wheat (Triticum aestivum and T. durum) caused by the necrotrophic fungal pathogen Parastagonospora nodorum. SNB resistance is a typical quantitative trait, controlled by multiple quantitative trait loci (QTL) of minor effect. To achieve increased plant resistance, selection for resistance alleles and/or selection against susceptibility alleles must be undertaken. Here, we performed genetic analysis of SNB resistance using an eight-founder German Multiparent Advanced Generation Inter-Cross (MAGIC) population, termed BMWpop. Field trials and greenhouse testing were conducted over three seasons in Norway, with genetic analysis identifying ten SNB resistance QTL. Of these, two QTL were identified over two seasons: QSnb.nmbu-2A.1 on chromosome 2A and QSnb.nmbu-5A.1 on chromosome 5A. The chromosome 2A BMWpop QTL co-located with a robust SNB resistance QTL recently identified in an independent eight-founder MAGIC population constructed using varieties released in the United Kingdom (UK). The validation of this SNB resistance QTL in two independent multi-founder mapping populations, regardless of the differences in genetic background and agricultural environment, highlights the value of this locus in SNB resistance breeding. The second robust QTL identified in the BMWpop, QSnb.nmbu-5A.1, was not identified in the UK MAGIC population. Combining resistance alleles at both loci resulted in additive effects on SNB resistance. Therefore, using marker assisted selection to combine resistance alleles is a promising strategy for improving SNB resistance in wheat breeding. Indeed, the multi-locus haplotypes determined in this study provide markers for efficient tracking of these beneficial alleles in future wheat genetics and breeding activities.

Authors+Show Affiliations

Department of Plant Sciences, Norwegian University of Life Sciences, Post Box 5003, 1432, Ås, Norway.Bavarian State Research Center for Agriculture, Institute for Crop Science and Plant Breeding, Freising, Germany.Bavarian State Research Center for Agriculture, Institute for Crop Science and Plant Breeding, Freising, Germany.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Norwegian Institute of Bioeconomy Research, Høgskoleveien 7, 1433, Ås, Norway.John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.Department of Plant Sciences, Norwegian University of Life Sciences, Post Box 5003, 1432, Ås, Norway. morten.lillemo@nmbu.no.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33047219

Citation

Lin, Min, et al. "Identification and Cross-validation of Genetic Loci Conferring Resistance to Septoria Nodorum Blotch Using a German Multi-founder Winter Wheat Population." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 1, 2021, pp. 125-142.
Lin M, Stadlmeier M, Mohler V, et al. Identification and cross-validation of genetic loci conferring resistance to Septoria nodorum blotch using a German multi-founder winter wheat population. Theor Appl Genet. 2021;134(1):125-142.
Lin, M., Stadlmeier, M., Mohler, V., Tan, K. C., Ficke, A., Cockram, J., & Lillemo, M. (2021). Identification and cross-validation of genetic loci conferring resistance to Septoria nodorum blotch using a German multi-founder winter wheat population. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(1), 125-142. https://doi.org/10.1007/s00122-020-03686-x
Lin M, et al. Identification and Cross-validation of Genetic Loci Conferring Resistance to Septoria Nodorum Blotch Using a German Multi-founder Winter Wheat Population. Theor Appl Genet. 2021;134(1):125-142. PubMed PMID: 33047219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and cross-validation of genetic loci conferring resistance to Septoria nodorum blotch using a German multi-founder winter wheat population. AU - Lin,Min, AU - Stadlmeier,Melanie, AU - Mohler,Volker, AU - Tan,Kar-Chun, AU - Ficke,Andrea, AU - Cockram,James, AU - Lillemo,Morten, Y1 - 2020/10/12/ PY - 2020/04/03/received PY - 2020/09/12/accepted PY - 2020/10/14/pubmed PY - 2021/6/11/medline PY - 2020/10/13/entrez SP - 125 EP - 142 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 1 N2 - KEY MESSAGE: We identified allelic variation at two major loci, QSnb.nmbu-2A.1 and QSnb.nmbu-5A.1, showing consistent and additive effects on SNB field resistance. Validation of QSnb.nmbu-2A.1 across genetic backgrounds further highlights its usefulness for marker-assisted selection. Septoria nodorum blotch (SNB) is a disease of wheat (Triticum aestivum and T. durum) caused by the necrotrophic fungal pathogen Parastagonospora nodorum. SNB resistance is a typical quantitative trait, controlled by multiple quantitative trait loci (QTL) of minor effect. To achieve increased plant resistance, selection for resistance alleles and/or selection against susceptibility alleles must be undertaken. Here, we performed genetic analysis of SNB resistance using an eight-founder German Multiparent Advanced Generation Inter-Cross (MAGIC) population, termed BMWpop. Field trials and greenhouse testing were conducted over three seasons in Norway, with genetic analysis identifying ten SNB resistance QTL. Of these, two QTL were identified over two seasons: QSnb.nmbu-2A.1 on chromosome 2A and QSnb.nmbu-5A.1 on chromosome 5A. The chromosome 2A BMWpop QTL co-located with a robust SNB resistance QTL recently identified in an independent eight-founder MAGIC population constructed using varieties released in the United Kingdom (UK). The validation of this SNB resistance QTL in two independent multi-founder mapping populations, regardless of the differences in genetic background and agricultural environment, highlights the value of this locus in SNB resistance breeding. The second robust QTL identified in the BMWpop, QSnb.nmbu-5A.1, was not identified in the UK MAGIC population. Combining resistance alleles at both loci resulted in additive effects on SNB resistance. Therefore, using marker assisted selection to combine resistance alleles is a promising strategy for improving SNB resistance in wheat breeding. Indeed, the multi-locus haplotypes determined in this study provide markers for efficient tracking of these beneficial alleles in future wheat genetics and breeding activities. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/33047219/Identification_and_cross_validation_of_genetic_loci_conferring_resistance_to_Septoria_nodorum_blotch_using_a_German_multi_founder_winter_wheat_population_ L2 - https://dx.doi.org/10.1007/s00122-020-03686-x DB - PRIME DP - Unbound Medicine ER -