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[Detection and evaluation of SARS-CoV-2 nucleic acid contamination in corona virus disease 19 ward surroundings and the surface of medical staff's protective equipment].
Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Oct 18; 52(5):803-808.BD

Abstract

OBJECTIVE

To determine the environmental contamination degree of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in corona virus disease 2019 (COVID-19) wards, to offer gui-dance for the infection control and to improve safety practices for medical staff, by sampling and detecting SARS-CoV-2 nucleic acid from the air of hospital wards, the high-frequency contact surfaces in the contaminated area and the surfaces of medical staff's protective equipment in a COVID-19 designated hospital in Wuhan, China.

METHODS

From March 11 to March 19, 2020, we collected air samples from the clean area, the buffer room and the contaminated area respectively in the COVID-19 wards using a portable bioaerosol concentrator WA-15. And sterile premoistened swabs were used to sample the high-frequency contacted surfaces in the contaminated area and the surfaces of medical staff's protective equipment including outermost gloves, tracheotomy operator's positive pressure respiratory protective hood and isolation clothing. The SARS-CoV-2 nucleic acid of the samples were detected by real-time fluorescence quantitative PCR. During the isolation medical observation period, those medical staff who worked in the COVID-19 wards were detected for SARS-CoV-2 nucleic acid with oropharyngeal swabs, IgM and IgG antibody in the sera, and chest CT scans to confirm the infection status of COVID-19.

RESULTS

No SARS-CoV-2 nucleic acid was detected in the tested samples, including the 90 air samples from the COVID-19 wards including clean area, buffer room and contaminated area, the 38 high-frequency contact surfaces samples of the contaminated area and 16 surface samples of medical staff's protective equipment including outermost gloves and isolation clothing. Moreover, detection of SARS-CoV-2 nucleic acid by oropharyngeal swabs and IgM, IgG antibodies in the sera of all the health-care workers who participated in the treatment for COVID-19 were all negative. Besides, no chest CT scan images of medical staff exhibited COVID-19 lung presentations.

CONCLUSION

Good ventilation conditions, strict disinfection of environmental facilities in hospital wards, guidance for correct habits in patients, and strict hand hygiene during medical staff are important to reduce the formation of viral aerosols, cut down the aerosol load, and avoid cross-infection in isolation wards. In the face of infectious diseases that were not fully mastered but ma-naged as class A, it is safe for medical personnel to be equipped at a high level.

Authors+Show Affiliations

Department of Hospital Infection Control, Peking University Third Hospital, Beijing 100191, China.Institute of Sports Medicine, Peking University Third Hospital, Beijing 100191, China.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing 100191, China.Department of Intensive Care Unit, Peking University Third Hospital, Beijing 100191, China.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing 100191, China.Department of Emergency Medicine, Peking University Third Hospital, Beijing 100191, China.Department of Intensive Care Unit, Peking University Third Hospital, Beijing 100191, China.Department of Infectious Disease, Peking University Third Hospital, Beijing 100191, China.Department of Hospital Infection Control, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.Department of General Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33047711

Citation

Yuan, X N., et al. "[Detection and Evaluation of SARS-CoV-2 Nucleic Acid Contamination in Corona Virus Disease 19 Ward Surroundings and the Surface of Medical Staff's Protective Equipment]." Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, vol. 52, no. 5, 2020, pp. 803-808.
Yuan XN, Meng QY, Shen N, et al. [Detection and evaluation of SARS-CoV-2 nucleic acid contamination in corona virus disease 19 ward surroundings and the surface of medical staff's protective equipment]. Beijing Da Xue Xue Bao Yi Xue Ban. 2020;52(5):803-808.
Yuan, X. N., Meng, Q. Y., Shen, N., Li, Y. X., Liang, C., Cui, M., Ge, Q. G., Li, X. G., Tan, K., Chen, Q., Wang, J., & Zeng, X. Y. (2020). [Detection and evaluation of SARS-CoV-2 nucleic acid contamination in corona virus disease 19 ward surroundings and the surface of medical staff's protective equipment]. Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, 52(5), 803-808.
Yuan XN, et al. [Detection and Evaluation of SARS-CoV-2 Nucleic Acid Contamination in Corona Virus Disease 19 Ward Surroundings and the Surface of Medical Staff's Protective Equipment]. Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Oct 18;52(5):803-808. PubMed PMID: 33047711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection and evaluation of SARS-CoV-2 nucleic acid contamination in corona virus disease 19 ward surroundings and the surface of medical staff's protective equipment]. AU - Yuan,X N, AU - Meng,Q Y, AU - Shen,N, AU - Li,Y X, AU - Liang,C, AU - Cui,M, AU - Ge,Q G, AU - Li,X G, AU - Tan,K, AU - Chen,Q, AU - Wang,J, AU - Zeng,X Y, PY - 2020/10/13/entrez PY - 2020/10/14/pubmed PY - 2020/10/21/medline KW - Coronavirus infections KW - Disease transmission, infectious KW - Health personnel KW - Patient isolation KW - Pneumonia, viral SP - 803 EP - 808 JF - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JO - Beijing Da Xue Xue Bao Yi Xue Ban VL - 52 IS - 5 N2 - OBJECTIVE: To determine the environmental contamination degree of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in corona virus disease 2019 (COVID-19) wards, to offer gui-dance for the infection control and to improve safety practices for medical staff, by sampling and detecting SARS-CoV-2 nucleic acid from the air of hospital wards, the high-frequency contact surfaces in the contaminated area and the surfaces of medical staff's protective equipment in a COVID-19 designated hospital in Wuhan, China. METHODS: From March 11 to March 19, 2020, we collected air samples from the clean area, the buffer room and the contaminated area respectively in the COVID-19 wards using a portable bioaerosol concentrator WA-15. And sterile premoistened swabs were used to sample the high-frequency contacted surfaces in the contaminated area and the surfaces of medical staff's protective equipment including outermost gloves, tracheotomy operator's positive pressure respiratory protective hood and isolation clothing. The SARS-CoV-2 nucleic acid of the samples were detected by real-time fluorescence quantitative PCR. During the isolation medical observation period, those medical staff who worked in the COVID-19 wards were detected for SARS-CoV-2 nucleic acid with oropharyngeal swabs, IgM and IgG antibody in the sera, and chest CT scans to confirm the infection status of COVID-19. RESULTS: No SARS-CoV-2 nucleic acid was detected in the tested samples, including the 90 air samples from the COVID-19 wards including clean area, buffer room and contaminated area, the 38 high-frequency contact surfaces samples of the contaminated area and 16 surface samples of medical staff's protective equipment including outermost gloves and isolation clothing. Moreover, detection of SARS-CoV-2 nucleic acid by oropharyngeal swabs and IgM, IgG antibodies in the sera of all the health-care workers who participated in the treatment for COVID-19 were all negative. Besides, no chest CT scan images of medical staff exhibited COVID-19 lung presentations. CONCLUSION: Good ventilation conditions, strict disinfection of environmental facilities in hospital wards, guidance for correct habits in patients, and strict hand hygiene during medical staff are important to reduce the formation of viral aerosols, cut down the aerosol load, and avoid cross-infection in isolation wards. In the face of infectious diseases that were not fully mastered but ma-naged as class A, it is safe for medical personnel to be equipped at a high level. SN - 1671-167X UR - https://www.unboundmedicine.com/medline/citation/33047711/[Detection_and_evaluation_of_SARS_CoV_2_nucleic_acid_contamination_in_corona_virus_disease_19_ward_surroundings_and_the_surface_of_medical_staff's_protective_equipment]_ L2 - http://xuebao.bjmu.edu.cn/EN/Y2020/V52/I5/803 DB - PRIME DP - Unbound Medicine ER -