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Human oral microbiome dysbiosis as a novel non-invasive biomarker in detection of colorectal cancer.
Theranostics. 2020; 10(25):11595-11606.T

Abstract

Background:

The oral microbiome may play an important role in colorectal carcinogenesis. However, few studies have investigated the association between oral microbiome and the development of colorectal cancer (CRC). We aimed to investigate whether oral health-colorectal tumor association has an underlying microbial basis, in the quest for novel non-invasive biomarkers for CRC.

Methods:

We collected oral swab samples from 161 patients with CRC, 34 patients with colorectal adenoma (CRA), and 58 healthy volunteers. The oral microbiota was assessed using 16S rRNA sequencing. We characterized oral microbiome, identified microbial markers, constructed and validated colorectal tumor (CRA and CRC) classifier.

Results:

Oral microbial composition and diversity were significantly different among the three groups, and the CRA group had the highest diversity. Analysis of the functional potential of oral microbiota demonstrated that the pathway involving cell motility was overrepresented in the CRA and CRC groups relative to that in the healthy controls. Moreover, a random forest model was constructed based on oral microbial markers, which could distinguish the colorectal tumor groups from the healthy controls and achieve a powerful classification potential in the discovery and validation cohorts.

Conclusion:

This study suggests a potential association between oral microbiome dysbiosis and colorectal cancer. Oral microbiota-based biomarkers may be helpful in predicting the risks for the development of CRA and CRC.

Authors+Show Affiliations

Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China. Guangdong Provincial People's Hospital & Guangdong Academy of Medical Science, Guangdong Lung Cancer Institute, Guangzhou, China. School of Medicine, South China University of Technology, Guangzhou, China.Department of GI Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai, China.Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Pub Type(s)

Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

33052235

Citation

Zhang, Sheng, et al. "Human Oral Microbiome Dysbiosis as a Novel Non-invasive Biomarker in Detection of Colorectal Cancer." Theranostics, vol. 10, no. 25, 2020, pp. 11595-11606.
Zhang S, Kong C, Yang Y, et al. Human oral microbiome dysbiosis as a novel non-invasive biomarker in detection of colorectal cancer. Theranostics. 2020;10(25):11595-11606.
Zhang, S., Kong, C., Yang, Y., Cai, S., Li, X., Cai, G., & Ma, Y. (2020). Human oral microbiome dysbiosis as a novel non-invasive biomarker in detection of colorectal cancer. Theranostics, 10(25), 11595-11606. https://doi.org/10.7150/thno.49515
Zhang S, et al. Human Oral Microbiome Dysbiosis as a Novel Non-invasive Biomarker in Detection of Colorectal Cancer. Theranostics. 2020;10(25):11595-11606. PubMed PMID: 33052235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human oral microbiome dysbiosis as a novel non-invasive biomarker in detection of colorectal cancer. AU - Zhang,Sheng, AU - Kong,Cheng, AU - Yang,Yongzhi, AU - Cai,Sanjun, AU - Li,Xinxiang, AU - Cai,Guoxiang, AU - Ma,Yanlei, Y1 - 2020/09/18/ PY - 2020/06/15/received PY - 2020/09/02/accepted PY - 2020/10/14/entrez PY - 2020/10/15/pubmed PY - 2021/6/2/medline KW - 16S rRNA KW - colorectal adenomas KW - colorectal cancers KW - oral microbiome SP - 11595 EP - 11606 JF - Theranostics JO - Theranostics VL - 10 IS - 25 N2 - Background: The oral microbiome may play an important role in colorectal carcinogenesis. However, few studies have investigated the association between oral microbiome and the development of colorectal cancer (CRC). We aimed to investigate whether oral health-colorectal tumor association has an underlying microbial basis, in the quest for novel non-invasive biomarkers for CRC. Methods: We collected oral swab samples from 161 patients with CRC, 34 patients with colorectal adenoma (CRA), and 58 healthy volunteers. The oral microbiota was assessed using 16S rRNA sequencing. We characterized oral microbiome, identified microbial markers, constructed and validated colorectal tumor (CRA and CRC) classifier. Results: Oral microbial composition and diversity were significantly different among the three groups, and the CRA group had the highest diversity. Analysis of the functional potential of oral microbiota demonstrated that the pathway involving cell motility was overrepresented in the CRA and CRC groups relative to that in the healthy controls. Moreover, a random forest model was constructed based on oral microbial markers, which could distinguish the colorectal tumor groups from the healthy controls and achieve a powerful classification potential in the discovery and validation cohorts. Conclusion: This study suggests a potential association between oral microbiome dysbiosis and colorectal cancer. Oral microbiota-based biomarkers may be helpful in predicting the risks for the development of CRA and CRC. SN - 1838-7640 UR - https://www.unboundmedicine.com/medline/citation/33052235/Human_oral_microbiome_dysbiosis_as_a_novel_non_invasive_biomarker_in_detection_of_colorectal_cancer_ L2 - https://www.thno.org/v10p11595.htm DB - PRIME DP - Unbound Medicine ER -