Tags

Type your tag names separated by a space and hit enter

Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation.
Environ Sci Pollut Res Int. 2021 Feb; 28(7):8266-8280.ES

Abstract

Recently, research has contributed to better knowledge on the occurrence of pesticides in coastal water by identifying frequently detected substances, their concentration range and their acute and chronic toxicity for organisms. Pesticide pollution is of particular concern in France due to important agricultural activities and presence of several exoreic catchment areas that vehicle pesticides up to coastal waters, impacting non-target marine species. Several ecotoxicology questions remain to be addressed concerning the long-term effects of chronic pesticide exposure and the mechanisms involved in adaptation to chemical stress. In the present study, we brought new insights on the genetic and epigenetic effects of the herbicide diuron in oyster genitors. During gametogenesis, we exposed Crassostrea gigas to environmentally realistic herbicide concentrations (0.2-0.3 μg L-1 during two 7-day periods at half-course and end of gametogenesis). Diuron exposure was shown to decrease global DNA methylation and total methyltransferase activity in whole oyster tissue; this is consistent with the previous observation of a significant decrease in DNMT1 gene expression. Diuron effect seemed to be tissue-specific; hypermethylation was detected in the digestive gland, whereas diuron exposure had no effect on gill and gonad tissue. The genotoxicity of diuron was confirmed by the detection of one adduct in gonad DNA. By using in vitro approaches and human DNMT1 (DNMT1 has not been purified yet in bivalves), the presence of DNA lesions (adduct, 8-oxodGuo) was shown to interfere with DNMT1 activity, indicating a complex interaction between DNA damage and DNA methylation. Based on our results, we propose mechanisms to explain the effect of diuron exposure on DNA methylation, a widespread epigenetic mark.

Authors+Show Affiliations

Ifremer, Ecotoxicology Laboratory, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes Cedex 03, France. fakcha@ifremer.fr.Ifremer, Ecotoxicology Laboratory, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes Cedex 03, France.Ifremer, UMR 5244, IHPE Interactions Hosts Pathogens Environment, UPVD, CNRS, University of Montpellier, CC 80, 34095, Montpellier, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33052562

Citation

Akcha, Farida, et al. "Genotoxic and Epigenetic Effects of Diuron in the Pacific Oyster: in Vitro Evidence of Interaction Between DNA Damage and DNA Methylation." Environmental Science and Pollution Research International, vol. 28, no. 7, 2021, pp. 8266-8280.
Akcha F, Barranger A, Bachère E. Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation. Environ Sci Pollut Res Int. 2021;28(7):8266-8280.
Akcha, F., Barranger, A., & Bachère, E. (2021). Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation. Environmental Science and Pollution Research International, 28(7), 8266-8280. https://doi.org/10.1007/s11356-020-11021-6
Akcha F, Barranger A, Bachère E. Genotoxic and Epigenetic Effects of Diuron in the Pacific Oyster: in Vitro Evidence of Interaction Between DNA Damage and DNA Methylation. Environ Sci Pollut Res Int. 2021;28(7):8266-8280. PubMed PMID: 33052562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation. AU - Akcha,Farida, AU - Barranger,Audrey, AU - Bachère,Evelyne, Y1 - 2020/10/14/ PY - 2020/04/15/received PY - 2020/09/27/accepted PY - 2020/10/15/pubmed PY - 2020/10/15/medline PY - 2020/10/14/entrez KW - 8-oxodGuo KW - DNA adducts KW - DNA damage KW - DNA methylation KW - Diuron KW - Genotoxicity KW - Oyster KW - Pesticide SP - 8266 EP - 8280 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 28 IS - 7 N2 - Recently, research has contributed to better knowledge on the occurrence of pesticides in coastal water by identifying frequently detected substances, their concentration range and their acute and chronic toxicity for organisms. Pesticide pollution is of particular concern in France due to important agricultural activities and presence of several exoreic catchment areas that vehicle pesticides up to coastal waters, impacting non-target marine species. Several ecotoxicology questions remain to be addressed concerning the long-term effects of chronic pesticide exposure and the mechanisms involved in adaptation to chemical stress. In the present study, we brought new insights on the genetic and epigenetic effects of the herbicide diuron in oyster genitors. During gametogenesis, we exposed Crassostrea gigas to environmentally realistic herbicide concentrations (0.2-0.3 μg L-1 during two 7-day periods at half-course and end of gametogenesis). Diuron exposure was shown to decrease global DNA methylation and total methyltransferase activity in whole oyster tissue; this is consistent with the previous observation of a significant decrease in DNMT1 gene expression. Diuron effect seemed to be tissue-specific; hypermethylation was detected in the digestive gland, whereas diuron exposure had no effect on gill and gonad tissue. The genotoxicity of diuron was confirmed by the detection of one adduct in gonad DNA. By using in vitro approaches and human DNMT1 (DNMT1 has not been purified yet in bivalves), the presence of DNA lesions (adduct, 8-oxodGuo) was shown to interfere with DNMT1 activity, indicating a complex interaction between DNA damage and DNA methylation. Based on our results, we propose mechanisms to explain the effect of diuron exposure on DNA methylation, a widespread epigenetic mark. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/33052562/Genotoxic_and_epigenetic_effects_of_diuron_in_the_Pacific_oyster:_in_vitro_evidence_of_interaction_between_DNA_damage_and_DNA_methylation_ L2 - https://dx.doi.org/10.1007/s11356-020-11021-6 DB - PRIME DP - Unbound Medicine ER -