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Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina.
Med Vet Entomol. 2021 06; 35(2):225-229.MV

Abstract

Antibiotic-resistant bacteria pose a major threat to global health in the 21st century, requiring a quick, cheap and effective response from public health officials. This study evaluated the antimicrobial activity of native excretions/secretions (NES) produced by third instar (3 days old) larvae of Calliphora vicina using a protocol adapted from the Institute of Clinical and Laboratory Standards (CLSI). The microorganisms tested were: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and the fungus Candida albicans. After the incubation period, the suspensions were diluted and spread on nutrient agar plates to count the colony-forming units. A turbidimetric test also was carried out to test the action of the NES of C. vicina against S. aureus, a very common bacterial species, with an enormous capacity for adaption and resistance, being one of the bacteria of medical importance that causes the most hospital and community infections in the world. According to our results, the NES of C. vicina exhibits antimicrobial activity at different dilutions, being most effective against the gram-negative bacteria E. coli and K. pneumoniae.

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Authors+Show Affiliations

Dallavecchia DL
Microbiology Division, Department of Pathology, Faculty of Medicine, University of Porto - Alameda Prof. Hernani Monteiro, Porto, Portugal. CAPES Foundation, Ministry of Education of Brazil, Brasília, Brazil.
Ricardo E
Microbiology Division, Department of Pathology, Faculty of Medicine, University of Porto - Alameda Prof. Hernani Monteiro, Porto, Portugal. Center for Health Technology and Services Research - CINTESIS, University of Porto, Porto, Portugal.
da Silva AS
Department of Quantitative Methods, Federal University of the State of Rio de Janeiro - UNIRIO, Rio de Janeiro, Brazil.
Rodrigues AG
Microbiology Division, Department of Pathology, Faculty of Medicine, University of Porto - Alameda Prof. Hernani Monteiro, Porto, Portugal. Center for Health Technology and Services Research - CINTESIS, University of Porto, Porto, Portugal. Burn Unit, São João Hospital Center, Porto, Portugal.

MeSH

AnimalsAnti-Bacterial AgentsAntifungal AgentsBiological ProductsCalliphoridaeCandida albicansDipteraEscherichia coliGram-Negative BacteriaKlebsiella pneumoniaeLarvaMicrobial Sensitivity TestsStaphylococcus aureus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33063897

Citation

Dallavecchia, D L., et al. "Antibacterial and Antifungal Activity of Excretions and Secretions of Calliphora Vicina." Medical and Veterinary Entomology, vol. 35, no. 2, 2021, pp. 225-229.
Dallavecchia DL, Ricardo E, da Silva AS, et al. Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina. Med Vet Entomol. 2021;35(2):225-229.
Dallavecchia, D. L., Ricardo, E., da Silva, A. S., & Rodrigues, A. G. (2021). Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina. Medical and Veterinary Entomology, 35(2), 225-229. https://doi.org/10.1111/mve.12486
Dallavecchia DL, et al. Antibacterial and Antifungal Activity of Excretions and Secretions of Calliphora Vicina. Med Vet Entomol. 2021;35(2):225-229. PubMed PMID: 33063897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina. AU - Dallavecchia,D L, AU - Ricardo,E, AU - da Silva,A S, AU - Rodrigues,A G, Y1 - 2020/10/16/ PY - 2020/09/13/revised PY - 2020/02/04/received PY - 2020/09/25/accepted PY - 2020/10/17/pubmed PY - 2021/5/26/medline PY - 2020/10/16/entrez KW - antimicrobial activity KW - calliphorid larvae KW - native excretions/secretions (NES) KW - natural antibiotics SP - 225 EP - 229 JF - Medical and veterinary entomology JO - Med Vet Entomol VL - 35 IS - 2 N2 - Antibiotic-resistant bacteria pose a major threat to global health in the 21st century, requiring a quick, cheap and effective response from public health officials. This study evaluated the antimicrobial activity of native excretions/secretions (NES) produced by third instar (3 days old) larvae of Calliphora vicina using a protocol adapted from the Institute of Clinical and Laboratory Standards (CLSI). The microorganisms tested were: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and the fungus Candida albicans. After the incubation period, the suspensions were diluted and spread on nutrient agar plates to count the colony-forming units. A turbidimetric test also was carried out to test the action of the NES of C. vicina against S. aureus, a very common bacterial species, with an enormous capacity for adaption and resistance, being one of the bacteria of medical importance that causes the most hospital and community infections in the world. According to our results, the NES of C. vicina exhibits antimicrobial activity at different dilutions, being most effective against the gram-negative bacteria E. coli and K. pneumoniae. SN - 1365-2915 UR - https://www.unboundmedicine.com/medline/citation/33063897/Antibacterial_and_antifungal_activity_of_excretions_and_secretions_of_Calliphora_vicina_ DB - PRIME DP - Unbound Medicine ER -