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[Effect of NF-κB Inhibitor PDTC on Proliferation and Apoptosis of Acute Leukemia Cell HL-60].
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Oct; 28(5):1534-1538.ZS

Abstract

OBJECTIVE

To explore the effect of nuclear factor kappa-B (NF-κB) inhibitor pyrrolidine dithiocarbamate (PDTC) on the proliferation and apoptosis of acute leukemia cell HL-60.

METHODS

HL-60 cells were cultured with PDTC of 0, 25, 50, 100 μmol/L for 24, 48, 72 h. The inhibition rate of cell proliferation was detected by CCK-8 assay. Cell apoptosis was detected by Hoechst staining. Cell cycle was detected by flow cytometry. The expression of B-cell lymphoma-2 (BCL-2), BCL-2 associated X protein (BAX), cyclinD1, activated cysteinyl aspartate specific proteinase (cleaved caspase 3), cleaved caspase 8 and activation of NF-κB signal pathway related protein was detected by Western blot.

RESULTS

After the HL-60 cells were cultured with PDTC of 25, 50, 100 μmol/L for 24, 48, 72 h, the inhibition rate of cell proliferation increased with the enhancement of PDTC concentration at the same time point (r=0.924, P<0.01). At the same PDTC concentration, the inhibition rate of cell proliferation increased with prolonging of time (r=0.952, P<0.01). After HL-60 cell was cultured with PDTC of 25, 50, 100 μmol/L for 48 h, compared with control group, PDTC of 25, 50, 100 μmol/L increased the cell apoptotic rate, arrested cell cycle at G1 phase (P<0.01), the expression of BCL-2, cyclinD1 and p-NF-κB p65 was down-regulated(P<0.05), the expression of BAX, cleaved caspase 3, cleaved caspase 8 was up-regulated(P<0.01). PDTC of 50, 100 μmol/L down-regulated the expression of p-inhibitor of NF-κB (p-IκBα)(P<0.01).

CONCLUSION

PDTC can inhibit acute leukemia HL-60 cell proliferation and induce cell apoptosis.

Authors+Show Affiliations

Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China.Department of Oncology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150000, Heilongjiang Province, China.Department of Radiology, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150000, Heilongjiang Province, China.Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China.Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China.Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China,E-mail: wyla568@163.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33067950

Citation

Tao, Shi, et al. "[Effect of NF-κB Inhibitor PDTC On Proliferation and Apoptosis of Acute Leukemia Cell HL-60]." Zhongguo Shi Yan Xue Ye Xue Za Zhi, vol. 28, no. 5, 2020, pp. 1534-1538.
Tao S, Xu SQ, Jiao MX, et al. [Effect of NF-κB Inhibitor PDTC on Proliferation and Apoptosis of Acute Leukemia Cell HL-60]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020;28(5):1534-1538.
Tao, S., Xu, S. Q., Jiao, M. X., Chen, Y., Fu, C. B., & Hu, M. (2020). [Effect of NF-κB Inhibitor PDTC on Proliferation and Apoptosis of Acute Leukemia Cell HL-60]. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 28(5), 1534-1538. https://doi.org/10.19746/j.cnki.issn.1009-2137.2020.05.018
Tao S, et al. [Effect of NF-κB Inhibitor PDTC On Proliferation and Apoptosis of Acute Leukemia Cell HL-60]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020;28(5):1534-1538. PubMed PMID: 33067950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of NF-κB Inhibitor PDTC on Proliferation and Apoptosis of Acute Leukemia Cell HL-60]. AU - Tao,Shi, AU - Xu,Shan-Qi, AU - Jiao,Ming-Xiu, AU - Chen,Yu, AU - Fu,Cai-Bo, AU - Hu,Min, PY - 2020/10/17/entrez PY - 2020/10/18/pubmed PY - 2020/10/21/medline SP - 1534 EP - 1538 JF - Zhongguo shi yan xue ye xue za zhi JO - Zhongguo Shi Yan Xue Ye Xue Za Zhi VL - 28 IS - 5 N2 - OBJECTIVE: To explore the effect of nuclear factor kappa-B (NF-κB) inhibitor pyrrolidine dithiocarbamate (PDTC) on the proliferation and apoptosis of acute leukemia cell HL-60. METHODS: HL-60 cells were cultured with PDTC of 0, 25, 50, 100 μmol/L for 24, 48, 72 h. The inhibition rate of cell proliferation was detected by CCK-8 assay. Cell apoptosis was detected by Hoechst staining. Cell cycle was detected by flow cytometry. The expression of B-cell lymphoma-2 (BCL-2), BCL-2 associated X protein (BAX), cyclinD1, activated cysteinyl aspartate specific proteinase (cleaved caspase 3), cleaved caspase 8 and activation of NF-κB signal pathway related protein was detected by Western blot. RESULTS: After the HL-60 cells were cultured with PDTC of 25, 50, 100 μmol/L for 24, 48, 72 h, the inhibition rate of cell proliferation increased with the enhancement of PDTC concentration at the same time point (r=0.924, P<0.01). At the same PDTC concentration, the inhibition rate of cell proliferation increased with prolonging of time (r=0.952, P<0.01). After HL-60 cell was cultured with PDTC of 25, 50, 100 μmol/L for 48 h, compared with control group, PDTC of 25, 50, 100 μmol/L increased the cell apoptotic rate, arrested cell cycle at G1 phase (P<0.01), the expression of BCL-2, cyclinD1 and p-NF-κB p65 was down-regulated(P<0.05), the expression of BAX, cleaved caspase 3, cleaved caspase 8 was up-regulated(P<0.01). PDTC of 50, 100 μmol/L down-regulated the expression of p-inhibitor of NF-κB (p-IκBα)(P<0.01). CONCLUSION: PDTC can inhibit acute leukemia HL-60 cell proliferation and induce cell apoptosis. SN - 1009-2137 UR - https://www.unboundmedicine.com/medline/citation/33067950/[Effect_of_NF_κB_Inhibitor_PDTC_on_Proliferation_and_Apoptosis_of_Acute_Leukemia_Cell_HL_60]_ L2 - https://medlineplus.gov/leukemia.html DB - PRIME DP - Unbound Medicine ER -