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[Effect of electroacupuncture preconditioning on the expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue of the rats with acute myocardial ischemia-reperfusion injury].
Zhongguo Zhen Jiu. 2020 Oct 12; 40(10):1103-7.ZZ

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) preconditioning on the expressions of nuclear transcription factors-kappa B (NF-κB) p65, NF-κB inhibitor (IκB) α and IκB kinase (IKK) β in rats with acute myocardial ischemia-reperfusion injury (MIRI) and to explore the mechanism of EA on heart meridian in relieving MIRI.

METHODS

A total of 40 SD rats were randomized into a sham-operation group, a model group, an EA heart meridian group and an EA lung meridian group, 10 rats in each one. In the EA heart meridian group, acupuncture was applied to "Shenmen" (HT 7) and "Tongli" (HT 5). In the EA lung meridian group, acupuncture was applied to "Taiyuan" (LU 9) and "Lieque" (LU 7). In these two groups, EA was exerted for 20 min each time, 1 V in voltage and 2 Hz in frequency once a day. A total of 7-day EA stimulation was required before model duplication. In the model group, the EA heart meridian group and the EA lung meridian group, using ligating left anterior descending coronary artery to establish the acute MIRI models. In the sham-operation group, the chest was open, but no ligation was exerted, just the needle was penetrated through the corresponding sites for one time. The electrocardiogram (ECG) was detected and ST segment displacement was analyzed. Using Western blot method, the relative expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue were determined in each group. Using ELISA method, the levels of serum IL-1β and IL-10 were determined in each group.

RESULTS

Compared with the sham-operation group, ST segment displacement value was elevated 30 min after ligating and reperfusion for 120 min in the model group (P<0.05), and the value in the EA heart meridian group was lower than the model group and the EA lung meridian group (P<0.05). Compared with the sham-operation group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were increased (P<0.05) and the expression of IκBα reduced in the rats of the model group (P<0.05). Compared with the model group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were reduced (P<0.05) and the expressions of IκBα increased in the rats of the EA heart meridian group and the EA lung meridian group (P<0.05). Compared with the EA lung meridian group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were reduced (P<0.05) and the expression of IκBα increased in the rats of the EA heart meridian group (P<0.05). Compared with the sham-operation group, the serum level of IL-1β was increased (P<0.05) and IL-10 reduced in the model group (P<0.05). Compared with the model group, the serum level of IL-1β was reduced (P<0.05) and IL-10 increased in the EA heart meridian group and the level of IL-1β was was reduced in the EA lung meridian group (P<0.05). Compared with the EA lung meridian group, the serum level of IL-1β was reduced (P<0.05) and IL-10 increased in the EA heart meridian group (P<0.05).

CONCLUSION

Electroacupuncture preconditioning at heart meridian acupoints obviously alleviates acute MIRI. IKK/IκB/NF-κB signaling pathway possibly participates in the protective mechanism of electroacupuncture preconditioning on acute MIRI.

Authors+Show Affiliations

School of Acupuncture-Moxibustion and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.School of Acupuncture-Moxibustion and Tuina, Anhui University of CM, Hefei 230012; Research Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of Chinese Medical Sciences, Hefei 230038.School of Acupuncture-Moxibustion and Tuina, Anhui University of CM, Hefei 230012; Research Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of Chinese Medical Sciences, Hefei 230038.School of Integrated Traditional and Western Medicine, Anhui University of CM.School of Acupuncture-Moxibustion and Tuina, Anhui University of CM, Hefei 230012; Research Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of Chinese Medical Sciences, Hefei 230038.School of Acupuncture-Moxibustion and Tuina, Anhui University of CM, Hefei 230012; Research Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of Chinese Medical Sciences, Hefei 230038.School of Acupuncture-Moxibustion and Tuina, Anhui University of CM, Hefei 230012; Research Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of Chinese Medical Sciences, Hefei 230038.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33068354

Citation

Hao, Feng, et al. "[Effect of Electroacupuncture Preconditioning On the Expressions of NF-κB P65, IκBα and IKKβ in Myocardial Tissue of the Rats With Acute Myocardial Ischemia-reperfusion Injury]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 40, no. 10, 2020, pp. 1103-7.
Hao F, Cai RL, Yu Q, et al. [Effect of electroacupuncture preconditioning on the expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue of the rats with acute myocardial ischemia-reperfusion injury]. Zhongguo Zhen Jiu. 2020;40(10):1103-7.
Hao, F., Cai, R. L., Yu, Q., Yin, G., Wang, J., Wu, Z. J., & Hu, L. (2020). [Effect of electroacupuncture preconditioning on the expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue of the rats with acute myocardial ischemia-reperfusion injury]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 40(10), 1103-7. https://doi.org/10.13703/j.0255-2930.20190811-k0004
Hao F, et al. [Effect of Electroacupuncture Preconditioning On the Expressions of NF-κB P65, IκBα and IKKβ in Myocardial Tissue of the Rats With Acute Myocardial Ischemia-reperfusion Injury]. Zhongguo Zhen Jiu. 2020 Oct 12;40(10):1103-7. PubMed PMID: 33068354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture preconditioning on the expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue of the rats with acute myocardial ischemia-reperfusion injury]. AU - Hao,Feng, AU - Cai,Rong-Lin, AU - Yu,Qing, AU - Yin,Gang, AU - Wang,Jie, AU - Wu,Zi-Jian, AU - Hu,Ling, PY - 2020/10/17/entrez PY - 2020/10/18/pubmed PY - 2021/1/7/medline KW - NF-κB KW - acute myocardial ischemia-reperfusion injury KW - electroacupuncture KW - heart meridian SP - 1103 EP - 7 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 40 IS - 10 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) preconditioning on the expressions of nuclear transcription factors-kappa B (NF-κB) p65, NF-κB inhibitor (IκB) α and IκB kinase (IKK) β in rats with acute myocardial ischemia-reperfusion injury (MIRI) and to explore the mechanism of EA on heart meridian in relieving MIRI. METHODS: A total of 40 SD rats were randomized into a sham-operation group, a model group, an EA heart meridian group and an EA lung meridian group, 10 rats in each one. In the EA heart meridian group, acupuncture was applied to "Shenmen" (HT 7) and "Tongli" (HT 5). In the EA lung meridian group, acupuncture was applied to "Taiyuan" (LU 9) and "Lieque" (LU 7). In these two groups, EA was exerted for 20 min each time, 1 V in voltage and 2 Hz in frequency once a day. A total of 7-day EA stimulation was required before model duplication. In the model group, the EA heart meridian group and the EA lung meridian group, using ligating left anterior descending coronary artery to establish the acute MIRI models. In the sham-operation group, the chest was open, but no ligation was exerted, just the needle was penetrated through the corresponding sites for one time. The electrocardiogram (ECG) was detected and ST segment displacement was analyzed. Using Western blot method, the relative expressions of NF-κB p65, IκBα and IKKβ in myocardial tissue were determined in each group. Using ELISA method, the levels of serum IL-1β and IL-10 were determined in each group. RESULTS: Compared with the sham-operation group, ST segment displacement value was elevated 30 min after ligating and reperfusion for 120 min in the model group (P<0.05), and the value in the EA heart meridian group was lower than the model group and the EA lung meridian group (P<0.05). Compared with the sham-operation group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were increased (P<0.05) and the expression of IκBα reduced in the rats of the model group (P<0.05). Compared with the model group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were reduced (P<0.05) and the expressions of IκBα increased in the rats of the EA heart meridian group and the EA lung meridian group (P<0.05). Compared with the EA lung meridian group, the expressions of NF-κB p65 and IKKβ in myocardial tissue were reduced (P<0.05) and the expression of IκBα increased in the rats of the EA heart meridian group (P<0.05). Compared with the sham-operation group, the serum level of IL-1β was increased (P<0.05) and IL-10 reduced in the model group (P<0.05). Compared with the model group, the serum level of IL-1β was reduced (P<0.05) and IL-10 increased in the EA heart meridian group and the level of IL-1β was was reduced in the EA lung meridian group (P<0.05). Compared with the EA lung meridian group, the serum level of IL-1β was reduced (P<0.05) and IL-10 increased in the EA heart meridian group (P<0.05). CONCLUSION: Electroacupuncture preconditioning at heart meridian acupoints obviously alleviates acute MIRI. IKK/IκB/NF-κB signaling pathway possibly participates in the protective mechanism of electroacupuncture preconditioning on acute MIRI. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/33068354/[Effect_of_electroacupuncture_preconditioning_on_the_expressions_of_NF_κB_p65_IκBα_and_IKKβ_in_myocardial_tissue_of_the_rats_with_acute_myocardial_ischemia_reperfusion_injury]_ DB - PRIME DP - Unbound Medicine ER -