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Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids.
Ecotoxicol Environ Saf. 2021 Jan 15; 208:111425.EE

Abstract

Hermaphroditic lumbricid Eisenia sp. earthworms are ubiquitous and highly resistant to a variety of environmental stressors, including heavy metals. Among the progeny of laboratory mated inter-specific pairs of Eisenia fetida (Ea) and Eisenia andrei (Ef) there are fertile Ha hybrids derived from Ea ova fertilized by Ef spermatozoa and very rare sterile Hf hybrids from Ef ova fertilized by Ea spermatozoa. The aim of the first part of the experiment was to compare the life traits and whole body accumulation of cadmium in adult earthworms from genetically defined Ea, Ef and their hybrids (Ha) exposed for four weeks to commercial soil either unpolluted (control) or cadmium-spiked leading to moderate (M) or high (H) soil pollution (M = 425 and H = 835 mg kg-1 dry soil weight). Such exposure impaired cocoon production but not affected earthworm viability despite the massive Cd bioaccumulation in the whole earthworm bodies reaching at M and H groups 316-454, 203-338, 114-253, and 377-309 mg kg-1 dry body weights of Ea, Ef1, Ef2, and Ha, respectively, surprisingly reaching maximum accumulation quantities in hybrids. The second part of the experiment aimed to investigate cadmium-related defense mechanisms at transcriptomic level in coelomocytes non-invasively extruded from coelomic cavities of the new sets of Ea, Ef, Ha, and Hf earthworms exposed to Cd in microcosms for 0 days (control), 2 days, and 7 days (M = 425 mg kg-1). Expression level of stress-induced Cd-metallothionein (mt) and superoxide dismutase (sod) were gradually up-regulated, while the immune-connected lysenin (lys) was rapidly down-regulated; the expression of glutathione S-transferase (gst) and phytochelatin synthase (pcs) remained unaffected. Mt and sod gene up-regulation and lys gene down-regulation were especially pronounced in Ea-derived hybrids. In sum, capacity of cadmium bioaccumulation and detoxification mechanisms is more efficient in interspecific hybrids than in the pure Ea and Ef species.

Authors+Show Affiliations

Univ. Lille, IMT Lille Douai, Univ. Artois, Yncrea Hauts-de-France, ULR4515 - LGCgE, Laboratoire de Génie Civil et géo-Environnement, F-59000 Lille, France; Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland.Univ. Lille, IMT Lille Douai, Univ. Artois, Yncrea Hauts-de-France, ULR4515 - LGCgE, Laboratoire de Génie Civil et géo-Environnement, F-59000 Lille, France. Electronic address: agnieszkarorat@gmail.com.Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland.Department of Comparative Anatomy, and Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland.Department of Comparative Anatomy, and Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland.Univ. Lille, IMT Lille Douai, Univ. Artois, Yncrea Hauts-de-France, ULR4515 - LGCgE, Laboratoire de Génie Civil et géo-Environnement, F-59000 Lille, France.Department of Comparative Anatomy, and Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33068978

Citation

Jaskulak, Marta, et al. "Species-specific Cd-detoxification Mechanisms in Lumbricid Earthworms Eisenia Andrei, Eisenia Fetida and Their Hybrids." Ecotoxicology and Environmental Safety, vol. 208, 2021, p. 111425.
Jaskulak M, Rorat A, Kurianska-Piatek L, et al. Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids. Ecotoxicol Environ Saf. 2021;208:111425.
Jaskulak, M., Rorat, A., Kurianska-Piatek, L., Hofman, S., Bigaj, J., Vandenbulcke, F., & Plytycz, B. (2021). Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids. Ecotoxicology and Environmental Safety, 208, 111425. https://doi.org/10.1016/j.ecoenv.2020.111425
Jaskulak M, et al. Species-specific Cd-detoxification Mechanisms in Lumbricid Earthworms Eisenia Andrei, Eisenia Fetida and Their Hybrids. Ecotoxicol Environ Saf. 2021 Jan 15;208:111425. PubMed PMID: 33068978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids. AU - Jaskulak,Marta, AU - Rorat,Agnieszka, AU - Kurianska-Piatek,Ligia, AU - Hofman,Sebastian, AU - Bigaj,Janusz, AU - Vandenbulcke,Franck, AU - Plytycz,Barbara, Y1 - 2020/10/15/ PY - 2020/07/09/received PY - 2020/09/08/revised PY - 2020/09/27/accepted PY - 2020/10/18/pubmed PY - 2021/1/8/medline PY - 2020/10/17/entrez KW - Bioaccumulation KW - Cadmium KW - Detoxification KW - Eisenia Sp. KW - Gene expression KW - Hybrids SP - 111425 EP - 111425 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 208 N2 - Hermaphroditic lumbricid Eisenia sp. earthworms are ubiquitous and highly resistant to a variety of environmental stressors, including heavy metals. Among the progeny of laboratory mated inter-specific pairs of Eisenia fetida (Ea) and Eisenia andrei (Ef) there are fertile Ha hybrids derived from Ea ova fertilized by Ef spermatozoa and very rare sterile Hf hybrids from Ef ova fertilized by Ea spermatozoa. The aim of the first part of the experiment was to compare the life traits and whole body accumulation of cadmium in adult earthworms from genetically defined Ea, Ef and their hybrids (Ha) exposed for four weeks to commercial soil either unpolluted (control) or cadmium-spiked leading to moderate (M) or high (H) soil pollution (M = 425 and H = 835 mg kg-1 dry soil weight). Such exposure impaired cocoon production but not affected earthworm viability despite the massive Cd bioaccumulation in the whole earthworm bodies reaching at M and H groups 316-454, 203-338, 114-253, and 377-309 mg kg-1 dry body weights of Ea, Ef1, Ef2, and Ha, respectively, surprisingly reaching maximum accumulation quantities in hybrids. The second part of the experiment aimed to investigate cadmium-related defense mechanisms at transcriptomic level in coelomocytes non-invasively extruded from coelomic cavities of the new sets of Ea, Ef, Ha, and Hf earthworms exposed to Cd in microcosms for 0 days (control), 2 days, and 7 days (M = 425 mg kg-1). Expression level of stress-induced Cd-metallothionein (mt) and superoxide dismutase (sod) were gradually up-regulated, while the immune-connected lysenin (lys) was rapidly down-regulated; the expression of glutathione S-transferase (gst) and phytochelatin synthase (pcs) remained unaffected. Mt and sod gene up-regulation and lys gene down-regulation were especially pronounced in Ea-derived hybrids. In sum, capacity of cadmium bioaccumulation and detoxification mechanisms is more efficient in interspecific hybrids than in the pure Ea and Ef species. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/33068978/Species_specific_Cd_detoxification_mechanisms_in_lumbricid_earthworms_Eisenia_andrei_Eisenia_fetida_and_their_hybrids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(20)31262-8 DB - PRIME DP - Unbound Medicine ER -