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NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam.
Pest Manag Sci. 2021 Feb; 77(2):677-685.PM

Abstract

BACKGROUND

The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D. citri has not been characterized and its function is undescribed.

RESULTS

The CPR gene of D. citri (DcCPR) was cloned and sequenced. The expression level of DcCPR, determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis, was highest in the midgut and in nymphs. After feeding on double-stranded RNA for 72 h, the DcCPR messenger RNA level in D. citri adults decreased by 68.4%, and the susceptibility of D. citri to imidacloprid and thiamethoxam significantly increased. Meanwhile, after DcCPR silencing, the specific activities of DcCPR protein and P450s were significantly reduced by 41.6% and 44.7%, respectively. The subsequent western blot analysis and quantification of band intensity also showed that DcCPR content significantly decreased, consistent with the results of the specific activity test. In a eukaryotic expression assay, the viability of cells expressing DcCPR was significantly higher than the viability of cells expressing green fluorescent protein (GFP) when cells were exposed to imidacloprid or thiamethoxam.

CONCLUSION

These results indicate that DcCPR contributes to D. citri susceptibility to imidacloprid and thiamethoxam.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yuan CY
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Jing TX
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Li W
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Liu XQ
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Liu TY
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Liu Y
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Chen ML
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Jiang RX
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Yuan GR
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Dou W
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Wang JJ
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China.

MeSH

AnimalsCitrusCytochrome P-450 Enzyme SystemHemipteraInsecticide ResistanceInsecticidesNADPH-Ferrihemoprotein ReductaseNeonicotinoidsNitro CompoundsThiamethoxam

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33073914

Citation

Yuan, Chen-Yang, et al. "NADPH-cytochrome P450 Reductase Mediates the Susceptibility of Asian Citrus Psyllid Diaphorina Citri to Imidacloprid and Thiamethoxam." Pest Management Science, vol. 77, no. 2, 2021, pp. 677-685.
Yuan CY, Jing TX, Li W, et al. NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam. Pest Manag Sci. 2021;77(2):677-685.
Yuan, C. Y., Jing, T. X., Li, W., Liu, X. Q., Liu, T. Y., Liu, Y., Chen, M. L., Jiang, R. X., Yuan, G. R., Dou, W., & Wang, J. J. (2021). NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam. Pest Management Science, 77(2), 677-685. https://doi.org/10.1002/ps.6143
Yuan CY, et al. NADPH-cytochrome P450 Reductase Mediates the Susceptibility of Asian Citrus Psyllid Diaphorina Citri to Imidacloprid and Thiamethoxam. Pest Manag Sci. 2021;77(2):677-685. PubMed PMID: 33073914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam. AU - Yuan,Chen-Yang, AU - Jing,Tian-Xing, AU - Li,Wei, AU - Liu,Xiao-Qiang, AU - Liu,Tian-Yuan, AU - Liu,Yi, AU - Chen,Meng-Ling, AU - Jiang,Rui-Xu, AU - Yuan,Guo-Rui, AU - Dou,Wei, AU - Wang,Jin-Jun, Y1 - 2020/11/02/ PY - 2020/08/06/received PY - 2020/10/14/revised PY - 2020/10/19/accepted PY - 2020/10/20/pubmed PY - 2021/1/15/medline PY - 2020/10/19/entrez KW - Diaphorina citri KW - NADPH-cytochrome P450 reductase KW - RNA interference KW - eukaryotic expression KW - imidacloprid KW - thiamethoxam SP - 677 EP - 685 JF - Pest management science JO - Pest Manag Sci VL - 77 IS - 2 N2 - BACKGROUND: The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D. citri has not been characterized and its function is undescribed. RESULTS: The CPR gene of D. citri (DcCPR) was cloned and sequenced. The expression level of DcCPR, determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis, was highest in the midgut and in nymphs. After feeding on double-stranded RNA for 72 h, the DcCPR messenger RNA level in D. citri adults decreased by 68.4%, and the susceptibility of D. citri to imidacloprid and thiamethoxam significantly increased. Meanwhile, after DcCPR silencing, the specific activities of DcCPR protein and P450s were significantly reduced by 41.6% and 44.7%, respectively. The subsequent western blot analysis and quantification of band intensity also showed that DcCPR content significantly decreased, consistent with the results of the specific activity test. In a eukaryotic expression assay, the viability of cells expressing DcCPR was significantly higher than the viability of cells expressing green fluorescent protein (GFP) when cells were exposed to imidacloprid or thiamethoxam. CONCLUSION: These results indicate that DcCPR contributes to D. citri susceptibility to imidacloprid and thiamethoxam. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/33073914/NADPH_cytochrome_P450_reductase_mediates_the_susceptibility_of_Asian_citrus_psyllid_Diaphorina_citri_to_imidacloprid_and_thiamethoxam_ DB - PRIME DP - Unbound Medicine ER -
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