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Ratiometric electrogenerated chemiluminescence sensor based on a designed anti-fouling peptide for the detection of carcinoembryonic antigen.
Anal Chim Acta. 2020 Nov 01; 1136:134-140.AC

Abstract

In this paper, an effective and accurate ratiometric electrochemiluminescence (ECL) system based on Au-luminol and CdS quantum dots (CdS QDs) as signal probes was constructed for detecting carcinoembryonic antigen (CEA). Polyaniline (PANI) and gold nanoparticles (AuNPs) strongly enhanced the electronic transfer efficiency and the specific area of the modified sensing surface, and improved the detection sensitivity. CdS QDs functionalized DNA strands functioned as cathode ECL emitters, and the aptamer of CEA modified with Au-luminol and quencher cyanine dye (Cy5) fluorophore functioned as anode ECL emitters. After the DNA capture probe combined with CEA aptamer, the negative potential ECL response of the CdS QDs was quenched because of electrochemiluminescence resonance energy transfer (ERET) between the Cy5 fluorophore and CdS QDs. However, the positive potential ECL response of Au-luminol can still be detected. The negative potential ECL response of CdS QDs was recovered, and the positive potential ECL response of Au-luminol decreased after CEA combined with its aptamer to take Cy5 and Au-luminol off the sensing interface. Additionally, the peptide provided effective anti-fouling performance in the detection of complex samples. The ratiometric ECL sensor with anti-fouling ability can sensitively determine the concentration of CEA in human serum.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hao Q
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Wang L
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Niu S
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Ding C
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address: dingcaifeng2003@163.com.
Luo X
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.

MeSH

Biosensing TechniquesCarcinoembryonic AntigenElectrochemical TechniquesGoldHumansLimit of DetectionLuminescenceLuminescent MeasurementsMetal NanoparticlesPeptidesQuantum Dots

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33081937

Citation

Hao, Qiuxia, et al. "Ratiometric Electrogenerated Chemiluminescence Sensor Based On a Designed Anti-fouling Peptide for the Detection of Carcinoembryonic Antigen." Analytica Chimica Acta, vol. 1136, 2020, pp. 134-140.
Hao Q, Wang L, Niu S, et al. Ratiometric electrogenerated chemiluminescence sensor based on a designed anti-fouling peptide for the detection of carcinoembryonic antigen. Anal Chim Acta. 2020;1136:134-140.
Hao, Q., Wang, L., Niu, S., Ding, C., & Luo, X. (2020). Ratiometric electrogenerated chemiluminescence sensor based on a designed anti-fouling peptide for the detection of carcinoembryonic antigen. Analytica Chimica Acta, 1136, 134-140. https://doi.org/10.1016/j.aca.2020.09.033
Hao Q, et al. Ratiometric Electrogenerated Chemiluminescence Sensor Based On a Designed Anti-fouling Peptide for the Detection of Carcinoembryonic Antigen. Anal Chim Acta. 2020 Nov 1;1136:134-140. PubMed PMID: 33081937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ratiometric electrogenerated chemiluminescence sensor based on a designed anti-fouling peptide for the detection of carcinoembryonic antigen. AU - Hao,Qiuxia, AU - Wang,Lei, AU - Niu,Shuyan, AU - Ding,Caifeng, AU - Luo,Xiliang, Y1 - 2020/09/25/ PY - 2020/06/23/received PY - 2020/09/15/revised PY - 2020/09/17/accepted PY - 2020/10/21/entrez PY - 2020/10/22/pubmed PY - 2021/5/15/medline KW - Anti-fouling peptide KW - Au-luminol KW - Carcinoembryonic antigen KW - CdS QDs KW - Electrogenerated chemiluminescence (ECL) KW - Ratiometric biosensor SP - 134 EP - 140 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1136 N2 - In this paper, an effective and accurate ratiometric electrochemiluminescence (ECL) system based on Au-luminol and CdS quantum dots (CdS QDs) as signal probes was constructed for detecting carcinoembryonic antigen (CEA). Polyaniline (PANI) and gold nanoparticles (AuNPs) strongly enhanced the electronic transfer efficiency and the specific area of the modified sensing surface, and improved the detection sensitivity. CdS QDs functionalized DNA strands functioned as cathode ECL emitters, and the aptamer of CEA modified with Au-luminol and quencher cyanine dye (Cy5) fluorophore functioned as anode ECL emitters. After the DNA capture probe combined with CEA aptamer, the negative potential ECL response of the CdS QDs was quenched because of electrochemiluminescence resonance energy transfer (ERET) between the Cy5 fluorophore and CdS QDs. However, the positive potential ECL response of Au-luminol can still be detected. The negative potential ECL response of CdS QDs was recovered, and the positive potential ECL response of Au-luminol decreased after CEA combined with its aptamer to take Cy5 and Au-luminol off the sensing interface. Additionally, the peptide provided effective anti-fouling performance in the detection of complex samples. The ratiometric ECL sensor with anti-fouling ability can sensitively determine the concentration of CEA in human serum. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/33081937/Ratiometric_electrogenerated_chemiluminescence_sensor_based_on_a_designed_anti_fouling_peptide_for_the_detection_of_carcinoembryonic_antigen_ DB - PRIME DP - Unbound Medicine ER -