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Modified self-obtained pooled sampling to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in men who have sex with men.
Sex Transm Infect. 2021 08; 97(5):324-328.ST

Abstract

OBJECTIVES

To assess whether pooled sample testing with nucleic acid amplification tests was a potential alternative to three single-site sample testing to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in asymptomatic men who have sex with men.

METHODS

We prospectively compared pooled sample testing with single-site sample testing in asymptomatic MSM. Self-obtained paired rectal samples, one gargle sample and one first-void urine sample were collected from participants to generate two sets of samples: one for pooled sample testing and the other for single-site testing. We used modified pooled sampling, which is defined as the use of gargle samples, instead of swabs, for the pooled sample to test for pharyngeal infection.

RESULTS

This study included 513 MSM. The positive rates of C. trachomatis and N. gonorrhoeae were 20.3% and 11.7%, respectively, for single-site sample testing. Compared with the sensitivity of single-site testing as the gold standard, the sensitivities of pooled sample testing for C. trachomatis and N. gonorrhoeae were 94.2% (95% CI 88.0% to 97.3%) and 98.3% (95% CI 90.9% to 99.9%), respectively. The concordance rate and kappa coefficient were 98.3% (95% CI 96.7% to 99.2%) and 0.945 (95% CI 0.859 to 1.000), respectively, for C. trachomatis and 98.8% (95% CI 90.1% to 100%) and 0.943 (95% CI 0.857 to 1.000), respectively, for N. gonorrhoeae.

CONCLUSIONS

The modified pooled sampling had a comparably high consistency with single-site sample testing. The results strongly suggest that the gargle sample is suitable as a part of pooled sample for STI screening of C. trachomatis and N. gonorrhoeae.

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Authors+Show Affiliations

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan. Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Kumamoto, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan. Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Kumamoto, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan. Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Kumamoto, Japan.

AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku-ku, Japan higatana@acc.ncgm.go.jp. Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Kumamoto, Japan.

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33082236

Citation

Ando, Naokatsu, et al. "Modified Self-obtained Pooled Sampling to Screen for Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections in Men Who Have Sex With Men." Sexually Transmitted Infections, vol. 97, no. 5, 2021, pp. 324-328.

Ando N, Mizushima D, Watanabe K, et al. Modified self-obtained pooled sampling to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in men who have sex with men. Sex Transm Infect. 2021;97(5):324-328.

Ando, N., Mizushima, D., Watanabe, K., Takano, M., Shiojiri, D., Uemura, H., Aoki, T., Yanagawa, Y., Kikuchi, Y., Oka, S., & Gatanaga, H. (2021). Modified self-obtained pooled sampling to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in men who have sex with men. Sexually Transmitted Infections, 97(5), 324-328. https://doi.org/10.1136/sextrans-2020-054666

Ando N, et al. Modified Self-obtained Pooled Sampling to Screen for Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections in Men Who Have Sex With Men. Sex Transm Infect. 2021;97(5):324-328. PubMed PMID: 33082236.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Modified self-obtained pooled sampling to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in men who have sex with men. AU - Ando,Naokatsu, AU - Mizushima,Daisuke, AU - Watanabe,Koji, AU - Takano,Misao, AU - Shiojiri,Daisuke, AU - Uemura,Haruka, AU - Aoki,Takahiro, AU - Yanagawa,Yasuaki, AU - Kikuchi,Yoshimi, AU - Oka,Shinichi, AU - Gatanaga,Hiroyuki, Y1 - 2020/10/20/ PY - 2020/06/23/received PY - 2020/08/14/revised PY - 2020/09/27/accepted PY - 2020/10/22/pubmed PY - 2020/10/22/medline PY - 2020/10/21/entrez KW - Chlamydia trachomatis KW - Neisseria gonorrhoeae KW - screening KW - sexual health SP - 324 EP - 328 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 97 IS - 5 N2 - OBJECTIVES: To assess whether pooled sample testing with nucleic acid amplification tests was a potential alternative to three single-site sample testing to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in asymptomatic men who have sex with men. METHODS: We prospectively compared pooled sample testing with single-site sample testing in asymptomatic MSM. Self-obtained paired rectal samples, one gargle sample and one first-void urine sample were collected from participants to generate two sets of samples: one for pooled sample testing and the other for single-site testing. We used modified pooled sampling, which is defined as the use of gargle samples, instead of swabs, for the pooled sample to test for pharyngeal infection. RESULTS: This study included 513 MSM. The positive rates of C. trachomatis and N. gonorrhoeae were 20.3% and 11.7%, respectively, for single-site sample testing. Compared with the sensitivity of single-site testing as the gold standard, the sensitivities of pooled sample testing for C. trachomatis and N. gonorrhoeae were 94.2% (95% CI 88.0% to 97.3%) and 98.3% (95% CI 90.9% to 99.9%), respectively. The concordance rate and kappa coefficient were 98.3% (95% CI 96.7% to 99.2%) and 0.945 (95% CI 0.859 to 1.000), respectively, for C. trachomatis and 98.8% (95% CI 90.1% to 100%) and 0.943 (95% CI 0.857 to 1.000), respectively, for N. gonorrhoeae. CONCLUSIONS: The modified pooled sampling had a comparably high consistency with single-site sample testing. The results strongly suggest that the gargle sample is suitable as a part of pooled sample for STI screening of C. trachomatis and N. gonorrhoeae. SN - 1472-3263 UR - https://www.unboundmedicine.com/medline/citation/33082236/Modified_self_obtained_pooled_sampling_to_screen_for_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_infections_in_men_who_have_sex_with_men_ L2 - https://sti.bmj.com/lookup/pmidlookup?view=long&pmid=33082236 DB - PRIME DP - Unbound Medicine ER -