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Antigenotoxic, antiproliferative and antimetastatic properties of a combination of native medicinal plants from Argentina.
J Ethnopharmacol. 2021 Mar 01; 267:113479.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Jarilla is the common name of an appreciated group of native plants from the semi-arid region in Argentina (Larrea cuneifolia Cav., Larrea divaricata Cav. and Zuccagnia punctata Cav.) that have been historically consumed to heal respiratory, musculoskeletal and skin ailments, as well as recommended for weakness/tiredness, hypertension, diabetes and cancer treatment. It was previously reported that some biological properties could be improved when these plants are used jointly. Infusions of a defined mixture, composed by three Jarilla species, L. cuneifolia: L. divaricata: Z. punctata (0.5:0.25:0.25) (HM2) showed synergistic and additive effect on antioxidant activity even after passing through the gastro-duodenal tract.

AIM OF THE STUDY

The main purpose of this work was to evaluate antigenotoxic, antitumor, and anti-metastatic properties of the Jarilla species that grow in the Northwest of Argentina and a herbal combination of them.

MATERIAL AND METHODS

Infusions of Jarilla mixture (HM2), and of each single plant species were prepared. Phenolic profiles of infusions were analyzed by HPLC-ESI-MS/MS and two relevant chemical markers were quantified. The antigenotoxic activity was evaluated by using the Ames test and the Cytokinesis-Block Micronucleus (CBMN) assay against direct mutagens. Evaluations of both cytotoxicity and antiproliferative effects were conducted on tumor and non-tumor cell lines. Both in vivo tumoral growth and metastasis inhibition were evaluated by using a carcinoma model on Balb/c mice.

RESULTS

HM2 mix could suppress genetic and chromosome mutations induced by 4-nitro-o-phenylendiamine (4-NPD) and doxorubicin. Herbal mixture and single plant infusions showed cytotoxic effect against mammary, uterus, and brain tumoral cells without a selective action vs normal human cell line. HM2 mix was able to reduce mammary tumor mass on the Balb/c mice model and showed a significant reduction in the number of metastatic nodules in the lungs.

CONCLUSIONS

Our results suggest that the combinations of three Jarilla species from northwest Argentina would be a promising alternative to treat or slow down the development of chronic diseases, such as cancer.

Authors+Show Affiliations

Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, San Lorenzo 1469, 4000, San Miguel de Tucumán, Tucumán, Argentina. Electronic address: carabajalantonella@gmail.com.Departamento de Toxicología Genética y Antitumorales, Centro de Investigación y Desarrollo de Medicamentos (CIDEM), Avenida 26, No. 1605 e/Puentes Grandes y Boyeros, La Habana, Cuba. Electronic address: janet.piloto@cidem.cu.Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil. Electronic address: helo_nicolella@hotmail.com.Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil. Electronic address: iasquarisi79@outlook.com.Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil. Electronic address: paulinhaguissoni@hotmail.com.Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil. Electronic address: tabataesperandim@hotmail.com.Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil. Electronic address: denisecrispim2001@yahoo.com.Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, San Lorenzo 1469, 4000, San Miguel de Tucumán, Tucumán, Argentina. Electronic address: misla@tucbbs.com.ar.Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, San Lorenzo 1469, 4000, San Miguel de Tucumán, Tucumán, Argentina. Electronic address: zampini@csnat.unt.edu.ar.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

33091491

Citation

Carabajal, Monica Patricia Antonella, et al. "Antigenotoxic, Antiproliferative and Antimetastatic Properties of a Combination of Native Medicinal Plants From Argentina." Journal of Ethnopharmacology, vol. 267, 2021, p. 113479.
Carabajal MPA, Piloto-Ferrer J, Nicollela HD, et al. Antigenotoxic, antiproliferative and antimetastatic properties of a combination of native medicinal plants from Argentina. J Ethnopharmacol. 2021;267:113479.
Carabajal, M. P. A., Piloto-Ferrer, J., Nicollela, H. D., Squarisi, I. S., Prado Guissone, A. P., Esperandim, T. R., Tavares, D. C., Isla, M. I., & Zampini, I. C. (2021). Antigenotoxic, antiproliferative and antimetastatic properties of a combination of native medicinal plants from Argentina. Journal of Ethnopharmacology, 267, 113479. https://doi.org/10.1016/j.jep.2020.113479
Carabajal MPA, et al. Antigenotoxic, Antiproliferative and Antimetastatic Properties of a Combination of Native Medicinal Plants From Argentina. J Ethnopharmacol. 2021 Mar 1;267:113479. PubMed PMID: 33091491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigenotoxic, antiproliferative and antimetastatic properties of a combination of native medicinal plants from Argentina. AU - Carabajal,Monica Patricia Antonella, AU - Piloto-Ferrer,Janet, AU - Nicollela,Heloiza Diniz, AU - Squarisi,Iara Silva, AU - Prado Guissone,Ana Paula, AU - Esperandim,Tábata Rodrigues, AU - Tavares,Denise Crispim, AU - Isla,María Inés, AU - Zampini,Iris Catiana, Y1 - 2020/10/20/ PY - 2020/07/14/received PY - 2020/10/08/revised PY - 2020/10/13/accepted PY - 2020/10/23/pubmed PY - 2021/3/3/medline PY - 2020/10/22/entrez KW - Antimetastatic effect KW - Antimutagenic KW - Antitumoral KW - Clonogenic KW - Larrea cuneifolia KW - Larrea divaricata KW - Micronuclei KW - Zuccagnia punctata SP - 113479 EP - 113479 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 267 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Jarilla is the common name of an appreciated group of native plants from the semi-arid region in Argentina (Larrea cuneifolia Cav., Larrea divaricata Cav. and Zuccagnia punctata Cav.) that have been historically consumed to heal respiratory, musculoskeletal and skin ailments, as well as recommended for weakness/tiredness, hypertension, diabetes and cancer treatment. It was previously reported that some biological properties could be improved when these plants are used jointly. Infusions of a defined mixture, composed by three Jarilla species, L. cuneifolia: L. divaricata: Z. punctata (0.5:0.25:0.25) (HM2) showed synergistic and additive effect on antioxidant activity even after passing through the gastro-duodenal tract. AIM OF THE STUDY: The main purpose of this work was to evaluate antigenotoxic, antitumor, and anti-metastatic properties of the Jarilla species that grow in the Northwest of Argentina and a herbal combination of them. MATERIAL AND METHODS: Infusions of Jarilla mixture (HM2), and of each single plant species were prepared. Phenolic profiles of infusions were analyzed by HPLC-ESI-MS/MS and two relevant chemical markers were quantified. The antigenotoxic activity was evaluated by using the Ames test and the Cytokinesis-Block Micronucleus (CBMN) assay against direct mutagens. Evaluations of both cytotoxicity and antiproliferative effects were conducted on tumor and non-tumor cell lines. Both in vivo tumoral growth and metastasis inhibition were evaluated by using a carcinoma model on Balb/c mice. RESULTS: HM2 mix could suppress genetic and chromosome mutations induced by 4-nitro-o-phenylendiamine (4-NPD) and doxorubicin. Herbal mixture and single plant infusions showed cytotoxic effect against mammary, uterus, and brain tumoral cells without a selective action vs normal human cell line. HM2 mix was able to reduce mammary tumor mass on the Balb/c mice model and showed a significant reduction in the number of metastatic nodules in the lungs. CONCLUSIONS: Our results suggest that the combinations of three Jarilla species from northwest Argentina would be a promising alternative to treat or slow down the development of chronic diseases, such as cancer. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/33091491/Antigenotoxic_antiproliferative_and_antimetastatic_properties_of_a_combination_of_native_medicinal_plants_from_Argentina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(20)33365-1 DB - PRIME DP - Unbound Medicine ER -