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Effect of Gloriosa superba linn (EEGS) on mPT and monosodium glutamate-induced proliferative disorder using rat model.
J Ethnopharmacol. 2021 Mar 01; 267:113498.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Hyperplasia, Tumors and cancers are various forms of proliferative disorders affecting humans. Surgery is the main treatment approach while other options are also associated with adverse effects. There is therefore a need for the development of better alternative therapy that is cost effective and readily available with little or no adverse effect. Some bioactive agents in medicinal plants exhibit their anti-proliferative potential by induction of mitochondrial permeability transition pore (mPT) opening. Gloriosa superba, a medicinal plant, is folklorically used in the treatment of tumors and cancers.

AIM OF THE STUDY

This study therefore aimed at investigating the effect of ethanol leaf extract of Gloriosa superba (EEGS) on mPT and monosodium glutamate-induced proliferative disorder in some specific tissues using rat model.

MATERIALS AND METHODS

Isolated rat liver mitochondria were exposed to different concentrations (10, 30, 50, 70 and 90 μg/ml) of EEGS. The mPT pore opening, cytochrome c release, mitochondrial ATPase activity and lipid peroxidation were assessed spectrophotometrically. Caspases 9 and 3 activities were carried out using ELISA technique. Histological assessment of the liver, prostate and uterus of normal and monosodium glutamate (MSG)-treated rats were carried out.

RESULTS

The results showed significant induction of mPT pore opening, release of cytochrome c, enhancement of mitochondrial ATPase activity, inhibition of lipid peroxidation and activation of caspases 9 and 3 activities by EEGS. The histological assessment revealed the presence of MSG-induced hepato-cellular damage, benign prostate hyperplasia and uterine hyperplasia which were ameliorated by EEGS co-administration.

CONCLUSIONS

These findings suggest that EEGS contains putative agents that can induce apoptosis via induction of mPT pore opening and as well protect against MSG-induced hepato-cellular damage and proliferative disorder in prostate and uterus.

Authors+Show Affiliations

Laboratory for Membrane Biochemistry Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria. Electronic address: mr_adeola@yahoo.com.Laboratory for Membrane Biochemistry Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria. Electronic address: funsoolorunsogo@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33091496

Citation

Olowofolahan, Adeola Oluwakemi, and Olufunso Olabode Olorunsogo. "Effect of Gloriosa Superba Linn (EEGS) On mPT and Monosodium Glutamate-induced Proliferative Disorder Using Rat Model." Journal of Ethnopharmacology, vol. 267, 2021, p. 113498.
Olowofolahan AO, Olorunsogo OO. Effect of Gloriosa superba linn (EEGS) on mPT and monosodium glutamate-induced proliferative disorder using rat model. J Ethnopharmacol. 2021;267:113498.
Olowofolahan, A. O., & Olorunsogo, O. O. (2021). Effect of Gloriosa superba linn (EEGS) on mPT and monosodium glutamate-induced proliferative disorder using rat model. Journal of Ethnopharmacology, 267, 113498. https://doi.org/10.1016/j.jep.2020.113498
Olowofolahan AO, Olorunsogo OO. Effect of Gloriosa Superba Linn (EEGS) On mPT and Monosodium Glutamate-induced Proliferative Disorder Using Rat Model. J Ethnopharmacol. 2021 Mar 1;267:113498. PubMed PMID: 33091496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Gloriosa superba linn (EEGS) on mPT and monosodium glutamate-induced proliferative disorder using rat model. AU - Olowofolahan,Adeola Oluwakemi, AU - Olorunsogo,Olufunso Olabode, Y1 - 2020/10/19/ PY - 2020/08/27/received PY - 2020/10/11/revised PY - 2020/10/15/accepted PY - 2020/10/23/pubmed PY - 2021/3/3/medline PY - 2020/10/22/entrez KW - Apoptosis KW - Gloriosa superba KW - Hyperplasia KW - Mitochondrial permeability transition pore KW - Monosodium glutamate SP - 113498 EP - 113498 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 267 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Hyperplasia, Tumors and cancers are various forms of proliferative disorders affecting humans. Surgery is the main treatment approach while other options are also associated with adverse effects. There is therefore a need for the development of better alternative therapy that is cost effective and readily available with little or no adverse effect. Some bioactive agents in medicinal plants exhibit their anti-proliferative potential by induction of mitochondrial permeability transition pore (mPT) opening. Gloriosa superba, a medicinal plant, is folklorically used in the treatment of tumors and cancers. AIM OF THE STUDY: This study therefore aimed at investigating the effect of ethanol leaf extract of Gloriosa superba (EEGS) on mPT and monosodium glutamate-induced proliferative disorder in some specific tissues using rat model. MATERIALS AND METHODS: Isolated rat liver mitochondria were exposed to different concentrations (10, 30, 50, 70 and 90 μg/ml) of EEGS. The mPT pore opening, cytochrome c release, mitochondrial ATPase activity and lipid peroxidation were assessed spectrophotometrically. Caspases 9 and 3 activities were carried out using ELISA technique. Histological assessment of the liver, prostate and uterus of normal and monosodium glutamate (MSG)-treated rats were carried out. RESULTS: The results showed significant induction of mPT pore opening, release of cytochrome c, enhancement of mitochondrial ATPase activity, inhibition of lipid peroxidation and activation of caspases 9 and 3 activities by EEGS. The histological assessment revealed the presence of MSG-induced hepato-cellular damage, benign prostate hyperplasia and uterine hyperplasia which were ameliorated by EEGS co-administration. CONCLUSIONS: These findings suggest that EEGS contains putative agents that can induce apoptosis via induction of mPT pore opening and as well protect against MSG-induced hepato-cellular damage and proliferative disorder in prostate and uterus. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/33091496/Effect_of_Gloriosa_superba_linn__EEGS__on_mPT_and_monosodium_glutamate_induced_proliferative_disorder_using_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(20)33384-5 DB - PRIME DP - Unbound Medicine ER -