Tags

Type your tag names separated by a space and hit enter

Development of a bio-inkjet printed LAMP test kit for detecting human African trypanosomiasis.
PLoS Negl Trop Dis. 2020 10; 14(10):e0008753.PN

Abstract

Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa. Early diagnosis and treatment prior to disease progression are crucial for the survival of HAT patients. We had previously established a loop-mediated isothermal amplification (LAMP) method for HAT diagnosis in which the reagents were dried for field-use purposes. In this study, we used a semi-automated process to produce the test tubes using a bio-inkjet printer to achieve an accurate production. The performance of the inkjet printer-produced dried LAMP test (CZC-LAMP) was found to be stable after storage for up to 180 days at 30 °C. The diagnostic accuracy of CZC-LAMP HAT was evaluated using DNA samples that were extracted from 116 Trypanosoma brucei gambiense patients and 66 T. b. rhodesiense patients. The sensitivity was 72% for T. b. gambiense (95%CI: 63%-80%) and 80% for T. b. rhodesiense (95%CI: 69%-89%). The specificity determined using DNA from 116 endemic control DNA samples was 95% (95%CI: 89%-98%). The performance of the CZC-LAMP HAT and CZC-LAMP rHAT were also evaluated using 14 crude blood lysate samples obtained from T. b. rhodesiense patients and endemic control samples collected from Rumphi District in Malawi. The sensitivity and specificity were both 100% (95%CI: 77%-100%). As the developed CZC-LAMP test does not require a cold chain or a sophisticated laboratory, it holds promise for use as a routine simple molecular tool for point-of-care HAT diagnosis in endemic areas.

Links

PMC Free PDF

Authors+Show Affiliations

Hayashida K
Division of Collaboration and Education, Research Center for Zoonosis Control (CZC), Hokkaido University, Sapporo, Japan. International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Nambala P
Department of Pathology, College of Medicine, University of Malawi, Blantyre, Malawi.
Reet NV
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerpen, Belgium.
Büscher P
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerpen, Belgium.
Kawai N
Division of Collaboration and Education, Research Center for Zoonosis Control (CZC), Hokkaido University, Sapporo, Japan.
Mutengo MM
Institute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, Lusaka, Zambia.
Musaya J
Department of Pathology, College of Medicine, University of Malawi, Blantyre, Malawi.
Namangala B
Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Sugimoto C
Division of Collaboration and Education, Research Center for Zoonosis Control (CZC), Hokkaido University, Sapporo, Japan.
Yamagishi J
Division of Collaboration and Education, Research Center for Zoonosis Control (CZC), Hokkaido University, Sapporo, Japan. International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.

MeSH

AnimalsDNA, ProtozoanHumansMalawiMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPoint-of-Care SystemsSensitivity and SpecificityTrypanosoma brucei gambienseTrypanosoma brucei rhodesienseTrypanosomiasis, African

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33091922

Citation

Hayashida, Kyoko, et al. "Development of a Bio-inkjet Printed LAMP Test Kit for Detecting Human African Trypanosomiasis." PLoS Neglected Tropical Diseases, vol. 14, no. 10, 2020, pp. e0008753.
Hayashida K, Nambala P, Reet NV, et al. Development of a bio-inkjet printed LAMP test kit for detecting human African trypanosomiasis. PLoS Negl Trop Dis. 2020;14(10):e0008753.
Hayashida, K., Nambala, P., Reet, N. V., Büscher, P., Kawai, N., Mutengo, M. M., Musaya, J., Namangala, B., Sugimoto, C., & Yamagishi, J. (2020). Development of a bio-inkjet printed LAMP test kit for detecting human African trypanosomiasis. PLoS Neglected Tropical Diseases, 14(10), e0008753. https://doi.org/10.1371/journal.pntd.0008753
Hayashida K, et al. Development of a Bio-inkjet Printed LAMP Test Kit for Detecting Human African Trypanosomiasis. PLoS Negl Trop Dis. 2020;14(10):e0008753. PubMed PMID: 33091922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a bio-inkjet printed LAMP test kit for detecting human African trypanosomiasis. AU - Hayashida,Kyoko, AU - Nambala,Peter, AU - Reet,Nick Van, AU - Büscher,Philippe, AU - Kawai,Naoko, AU - Mutengo,Mable Mwale, AU - Musaya,Janelisa, AU - Namangala,Boniface, AU - Sugimoto,Chihiro, AU - Yamagishi,Junya, Y1 - 2020/10/22/ PY - 2020/05/15/received PY - 2020/08/28/accepted PY - 2020/11/03/revised PY - 2020/10/23/pubmed PY - 2020/12/23/medline PY - 2020/10/22/entrez SP - e0008753 EP - e0008753 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 14 IS - 10 N2 - Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa. Early diagnosis and treatment prior to disease progression are crucial for the survival of HAT patients. We had previously established a loop-mediated isothermal amplification (LAMP) method for HAT diagnosis in which the reagents were dried for field-use purposes. In this study, we used a semi-automated process to produce the test tubes using a bio-inkjet printer to achieve an accurate production. The performance of the inkjet printer-produced dried LAMP test (CZC-LAMP) was found to be stable after storage for up to 180 days at 30 °C. The diagnostic accuracy of CZC-LAMP HAT was evaluated using DNA samples that were extracted from 116 Trypanosoma brucei gambiense patients and 66 T. b. rhodesiense patients. The sensitivity was 72% for T. b. gambiense (95%CI: 63%-80%) and 80% for T. b. rhodesiense (95%CI: 69%-89%). The specificity determined using DNA from 116 endemic control DNA samples was 95% (95%CI: 89%-98%). The performance of the CZC-LAMP HAT and CZC-LAMP rHAT were also evaluated using 14 crude blood lysate samples obtained from T. b. rhodesiense patients and endemic control samples collected from Rumphi District in Malawi. The sensitivity and specificity were both 100% (95%CI: 77%-100%). As the developed CZC-LAMP test does not require a cold chain or a sophisticated laboratory, it holds promise for use as a routine simple molecular tool for point-of-care HAT diagnosis in endemic areas. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/33091922/Development_of_a_bio_inkjet_printed_LAMP_test_kit_for_detecting_human_African_trypanosomiasis_ DB - PRIME DP - Unbound Medicine ER -