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Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal.
F1000Res. 2020; 9:379.F

Abstract

Background:

Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties.

Methods:

The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts.

Results:

Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC 50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC 50 of 104.2 and 125.9µg/ml, respectively.

Conclusions:

The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

Authors+Show Affiliations

Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal.Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33093944

Citation

Gauchan, Dhurva Prasad, et al. "Evaluation of Antimicrobial, Antioxidant and Cytotoxic Properties of Bioactive Compounds Produced From Endophytic Fungi of Himalayan Yew (Taxus Wallichiana) in Nepal." F1000Research, vol. 9, 2020, p. 379.
Gauchan DP, Kandel P, Tuladhar A, et al. Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal. F1000Res. 2020;9:379.
Gauchan, D. P., Kandel, P., Tuladhar, A., Acharya, A., Kadel, U., Baral, A., Shahi, A. B., & García-Gil, M. R. (2020). Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal. F1000Research, 9, 379. https://doi.org/10.12688/f1000research.23250.2
Gauchan DP, et al. Evaluation of Antimicrobial, Antioxidant and Cytotoxic Properties of Bioactive Compounds Produced From Endophytic Fungi of Himalayan Yew (Taxus Wallichiana) in Nepal. F1000Res. 2020;9:379. PubMed PMID: 33093944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal. AU - Gauchan,Dhurva Prasad, AU - Kandel,Pratistha, AU - Tuladhar,Astha, AU - Acharya,Ashesh, AU - Kadel,Upendra, AU - Baral,Aayush, AU - Shahi,Arjan Bir, AU - García-Gil,María Rosario, Y1 - 2020/05/19/ PY - 2020/09/25/accepted PY - 2020/10/26/entrez PY - 2020/10/27/pubmed PY - 2021/3/17/medline KW - DPPH KW - Endophytic KW - GAE KW - RE KW - Taxus wallichiana KW - cytotoxic SP - 379 EP - 379 JF - F1000Research JO - F1000Res VL - 9 N2 - Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC 50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC 50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents. SN - 2046-1402 UR - https://www.unboundmedicine.com/medline/citation/33093944/Evaluation_of_antimicrobial_antioxidant_and_cytotoxic_properties_of_bioactive_compounds_produced_from_endophytic_fungi_of_Himalayan_yew__Taxus_wallichiana__in_Nepal_ L2 - https://f1000research.com/articles/10.12688/f1000research.23250.2/doi DB - PRIME DP - Unbound Medicine ER -