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SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients.
Laryngoscope. 2021 05; 131(5):E1677-E1682.L

Abstract

OBJECTIVES/HYPOTHESIS

The emergence of a new coronavirus strain (SARS-CoV-2) in December 2019 from China led to a global pandemic. The lack of herd immunity against this virus and the possibility of viral spread from asymptomatic individuals is still a major challenge for the prevention of viral transmission. The aim of this study was to evaluate the presence of the virus in different bodily secretions as a potential source of viral spread among patients infected with SARS-CoV-2.

STUDY DESIGN

Cross Sectional Study.

METHODS

The study included 38 COVID-19 patients with a positive real-time polymerase chain reaction (RT-PCR) test result for SARS-CoV-2, obtained from the combined nasopharyngeal-oropharyngeal swab samples. Saliva, tear, and cerumen samples were taken from the patients within 72 hours of the first RT-PCR test. SARS-CoV-2 N1 and N2 gene regions were studied with single-step RT-PCR in all samples.

RESULTS

Among the studied samples, the highest positivity rate was in saliva (76.3%) followed by tears (55.3%) and cerumen (39.5%). Viral load in saliva was also significantly higher compared to tears and cerumen (P < .001), while there was no significant difference between tears and cerumen. Higher viral load in combined nasopharyngeal-oropharyngeal swab samples was associated with higher viral load in tears, but not in saliva or cerumen. Half of the saliva, tear, and cerumen samples obtained from asymptomatic patients contained SARS-CoV-2 genome.

CONCLUSIONS

The virus was detected in the saliva, tears, and cerumen samples of both symptomatic and asymptomatic patients. The potential role of these bodily fluids on viral spread needs to be studied.

LEVEL OF EVIDENCE

4 Laryngoscope, 131:E1677-E1682, 2021.

Authors+Show Affiliations

Department of Otorhinolaryngology and Head and Neck Surgery, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey. Drhanege Clinic, Istanbul, Turkey.Department of Medical Microbiology, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Otorhinolaryngology and Head and Neck Surgery, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Otorhinolaryngology and Head and Neck Surgery, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Infectious Diseases and Clinical Microbiology, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Ophthalmology, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Otorhinolaryngology and Head and Neck Surgery, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Physiology, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.Department of Infectious Diseases and Clinical Microbiology, Republic of Turkey Ministry of Health, Ankara, Turkey.Department of Infectious Diseases and Clinical Microbiology, Saglik Bilimleri University Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33094833

Citation

Hanege, Fatih M., et al. "SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients." The Laryngoscope, vol. 131, no. 5, 2021, pp. E1677-E1682.
Hanege FM, Kocoglu E, Kalcioglu MT, et al. SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients. Laryngoscope. 2021;131(5):E1677-E1682.
Hanege, F. M., Kocoglu, E., Kalcioglu, M. T., Celik, S., Cag, Y., Esen, F., Bayindir, E., Pence, S., Alp Mese, E., & Agalar, C. (2021). SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients. The Laryngoscope, 131(5), E1677-E1682. https://doi.org/10.1002/lary.29218
Hanege FM, et al. SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients. Laryngoscope. 2021;131(5):E1677-E1682. PubMed PMID: 33094833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 Presence in the Saliva, Tears, and Cerumen of COVID-19 Patients. AU - Hanege,Fatih M, AU - Kocoglu,Esra, AU - Kalcioglu,Mahmut T, AU - Celik,Serdal, AU - Cag,Yasemin, AU - Esen,Fehim, AU - Bayindir,Eray, AU - Pence,Sadrettin, AU - Alp Mese,Emine, AU - Agalar,Canan, Y1 - 2020/11/19/ PY - 2020/09/29/revised PY - 2020/08/31/received PY - 2020/10/19/accepted PY - 2020/10/24/pubmed PY - 2021/4/22/medline PY - 2020/10/23/entrez KW - COVID-19 KW - SARS-CoV-2 KW - cerumen KW - saliva KW - tear SP - E1677 EP - E1682 JF - The Laryngoscope JO - Laryngoscope VL - 131 IS - 5 N2 - OBJECTIVES/HYPOTHESIS: The emergence of a new coronavirus strain (SARS-CoV-2) in December 2019 from China led to a global pandemic. The lack of herd immunity against this virus and the possibility of viral spread from asymptomatic individuals is still a major challenge for the prevention of viral transmission. The aim of this study was to evaluate the presence of the virus in different bodily secretions as a potential source of viral spread among patients infected with SARS-CoV-2. STUDY DESIGN: Cross Sectional Study. METHODS: The study included 38 COVID-19 patients with a positive real-time polymerase chain reaction (RT-PCR) test result for SARS-CoV-2, obtained from the combined nasopharyngeal-oropharyngeal swab samples. Saliva, tear, and cerumen samples were taken from the patients within 72 hours of the first RT-PCR test. SARS-CoV-2 N1 and N2 gene regions were studied with single-step RT-PCR in all samples. RESULTS: Among the studied samples, the highest positivity rate was in saliva (76.3%) followed by tears (55.3%) and cerumen (39.5%). Viral load in saliva was also significantly higher compared to tears and cerumen (P < .001), while there was no significant difference between tears and cerumen. Higher viral load in combined nasopharyngeal-oropharyngeal swab samples was associated with higher viral load in tears, but not in saliva or cerumen. Half of the saliva, tear, and cerumen samples obtained from asymptomatic patients contained SARS-CoV-2 genome. CONCLUSIONS: The virus was detected in the saliva, tears, and cerumen samples of both symptomatic and asymptomatic patients. The potential role of these bodily fluids on viral spread needs to be studied. LEVEL OF EVIDENCE: 4 Laryngoscope, 131:E1677-E1682, 2021. SN - 1531-4995 UR - https://www.unboundmedicine.com/medline/citation/33094833/SARS_CoV_2_Presence_in_the_Saliva_Tears_and_Cerumen_of_COVID_19_Patients_ L2 - https://doi.org/10.1002/lary.29218 DB - PRIME DP - Unbound Medicine ER -