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Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics.
Nanomaterials (Basel). 2020 Oct 21; 10(10)N

Abstract

An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves of Mentha longifolia L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV-vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13-40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (-19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25-100 ppm) against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics.

Authors+Show Affiliations

Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.Department of Biosystems Engineering, Faculty of Environmental Engineering and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, Poland.Biology Department, Faculty of Science, Taibah University, Al-Sharm, Yanbu El-Bahr 46429, Saudi Arabia.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.National Research Centre, El-Behouth St., Dokki, Giza 12622, Egypt.Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33096854

Citation

Salem, Salem S., et al. "Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated By Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics." Nanomaterials (Basel, Switzerland), vol. 10, no. 10, 2020.
Salem SS, El-Belely EF, Niedbała G, et al. Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials (Basel). 2020;10(10).
Salem, S. S., El-Belely, E. F., Niedbała, G., Alnoman, M. M., Hassan, S. E., Eid, A. M., Shaheen, T. I., Elkelish, A., & Fouda, A. (2020). Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials (Basel, Switzerland), 10(10). https://doi.org/10.3390/nano10102082
Salem SS, et al. Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated By Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials (Basel). 2020 Oct 21;10(10) PubMed PMID: 33096854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. AU - Salem,Salem S, AU - El-Belely,Ehab F, AU - Niedbała,Gniewko, AU - Alnoman,Maryam M, AU - Hassan,Saad El-Din, AU - Eid,Ahmed Mohamed, AU - Shaheen,Tharwat I, AU - Elkelish,Amr, AU - Fouda,Amr, Y1 - 2020/10/21/ PY - 2020/10/01/received PY - 2020/10/16/revised PY - 2020/10/17/accepted PY - 2020/10/24/entrez PY - 2020/10/25/pubmed PY - 2020/10/25/medline KW - Ag-NPs KW - antibacterial and cytotoxicity activities KW - cotton fabrics KW - endophytic actinomycetes KW - green synthesis KW - streptomyces spp. JF - Nanomaterials (Basel, Switzerland) JO - Nanomaterials (Basel) VL - 10 IS - 10 N2 - An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves of Mentha longifolia L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV-vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13-40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (-19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25-100 ppm) against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics. SN - 2079-4991 UR - https://www.unboundmedicine.com/medline/citation/33096854/Bactericidal_and_In_Vitro_Cytotoxic_Efficacy_of_Silver_Nanoparticles__Ag_NPs__Fabricated_by_Endophytic_Actinomycetes_and_Their_Use_as_Coating_for_the_Textile_Fabrics_ L2 - https://www.mdpi.com/resolver?pii=nano10102082 DB - PRIME DP - Unbound Medicine ER -
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